Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or α-Ketoglutarate Supplementation in Rats Fed a High-Fat Diet
The aim of this study was to examine the impact of different dietary interventions started at middle age on the metabolic phenotype and gene expression profiling in the hypothalamus. One-year old rats were fed either a control diet, high-fat diet (HFD), HFD supplemented with resveratrol (HFD+RESV),...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Animal Reproduction and Food Research
2021-07-01
|
| Series: | Polish Journal of Food and Nutrition Sciences |
| Subjects: | |
| Online Access: | http://journal.pan.olsztyn.pl/Expression-Profile-of-Brain-Aging-and-Metabolic-Function-are-Altered-by-Resveratrol,139081,0,2.html |
| _version_ | 1818589707348475904 |
|---|---|
| author | Paulina Szczurek-Janicka Katarzyna Ropka-Molik Maria Oczkowicz Sylwia Orczewska-Dudek Mariusz Pietras Marek Pieszka |
| author_facet | Paulina Szczurek-Janicka Katarzyna Ropka-Molik Maria Oczkowicz Sylwia Orczewska-Dudek Mariusz Pietras Marek Pieszka |
| author_sort | Paulina Szczurek-Janicka |
| collection | DOAJ |
| description | The aim of this study was to examine the impact of different dietary interventions started at middle age on the metabolic phenotype and gene expression profiling in the hypothalamus. One-year old rats were fed either a control diet, high-fat diet (HFD), HFD supplemented with resveratrol (HFD+RESV), or HFD supplemented with α-ketoglutarate (HFD+AKG). A 6-week HFD feeding led to significant changes in concentrations of plasma glucose, insulin, lipids, and thyroid hormones. Moreover, 32% of the 84 analyzed genes correlated with aging were differentially expressed compared to the control group, with the largest functional class being related to inflammatory response. Dietary RESV ameliorated the changes in plasma glucose, total cholesterol, and triiodothyronine concentrations induced by HFD feeding and significantly downregulated 60% of the surveyed genes compared to the control group, resulting in a major molecular shift compared to HFD alone. In contrast, AKG supplementation did not affect the metabolic phenotype, but prevented the gene expression pattern caused by HFD consumption, mimicking the effects observed in the control group. HFD feeding induces metabolic dysfunction and age-related genetic alterations in the hypothalamus of middle-aged rats, while dietary RESV or AKG may partially retard these effects, even though these compounds act in a different and specific manner. |
| first_indexed | 2024-12-16T09:44:55Z |
| format | Article |
| id | doaj.art-0300ff0b676e4ebe81bd5d6b86c749da |
| institution | Directory Open Access Journal |
| issn | 2083-6007 |
| language | English |
| last_indexed | 2024-12-16T09:44:55Z |
| publishDate | 2021-07-01 |
| publisher | Institute of Animal Reproduction and Food Research |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj.art-0300ff0b676e4ebe81bd5d6b86c749da2022-12-21T22:36:11ZengInstitute of Animal Reproduction and Food ResearchPolish Journal of Food and Nutrition Sciences2083-60072021-07-0171325526810.31883/pjfns/139081139081Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or α-Ketoglutarate Supplementation in Rats Fed a High-Fat DietPaulina Szczurek-Janicka0Katarzyna Ropka-Molik1Maria Oczkowicz2Sylwia Orczewska-Dudek3Mariusz Pietras4Marek Pieszka5Department of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, Krakowska Str. 1, 32-083 Balice, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska Str. 1, 32-083 Balice, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska Str. 1, 32-083 Balice, PolandDepartment of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, Krakowska Str. 1, 32-083 Balice, PolandDepartment of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, Krakowska Str. 1, 32-083 Balice, PolandDepartment of Animal Nutrition and Feed Sciences, National Research Institute of Animal Production, Krakowska Str. 1, 32-083 Balice, PolandThe aim of this study was to examine the impact of different dietary interventions started at middle age on the metabolic phenotype and gene expression profiling in the hypothalamus. One-year old rats were fed either a control diet, high-fat diet (HFD), HFD supplemented with resveratrol (HFD+RESV), or HFD supplemented with α-ketoglutarate (HFD+AKG). A 6-week HFD feeding led to significant changes in concentrations of plasma glucose, insulin, lipids, and thyroid hormones. Moreover, 32% of the 84 analyzed genes correlated with aging were differentially expressed compared to the control group, with the largest functional class being related to inflammatory response. Dietary RESV ameliorated the changes in plasma glucose, total cholesterol, and triiodothyronine concentrations induced by HFD feeding and significantly downregulated 60% of the surveyed genes compared to the control group, resulting in a major molecular shift compared to HFD alone. In contrast, AKG supplementation did not affect the metabolic phenotype, but prevented the gene expression pattern caused by HFD consumption, mimicking the effects observed in the control group. HFD feeding induces metabolic dysfunction and age-related genetic alterations in the hypothalamus of middle-aged rats, while dietary RESV or AKG may partially retard these effects, even though these compounds act in a different and specific manner.http://journal.pan.olsztyn.pl/Expression-Profile-of-Brain-Aging-and-Metabolic-Function-are-Altered-by-Resveratrol,139081,0,2.htmlα-ketoglutaratebrain aginggene expression profilinghigh-fat dietresveratrolrats |
| spellingShingle | Paulina Szczurek-Janicka Katarzyna Ropka-Molik Maria Oczkowicz Sylwia Orczewska-Dudek Mariusz Pietras Marek Pieszka Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or α-Ketoglutarate Supplementation in Rats Fed a High-Fat Diet Polish Journal of Food and Nutrition Sciences α-ketoglutarate brain aging gene expression profiling high-fat diet resveratrol rats |
| title | Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or α-Ketoglutarate Supplementation in Rats Fed a High-Fat Diet |
| title_full | Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or α-Ketoglutarate Supplementation in Rats Fed a High-Fat Diet |
| title_fullStr | Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or α-Ketoglutarate Supplementation in Rats Fed a High-Fat Diet |
| title_full_unstemmed | Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or α-Ketoglutarate Supplementation in Rats Fed a High-Fat Diet |
| title_short | Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or α-Ketoglutarate Supplementation in Rats Fed a High-Fat Diet |
| title_sort | expression profile of brain aging and metabolic function are altered by resveratrol or α ketoglutarate supplementation in rats fed a high fat diet |
| topic | α-ketoglutarate brain aging gene expression profiling high-fat diet resveratrol rats |
| url | http://journal.pan.olsztyn.pl/Expression-Profile-of-Brain-Aging-and-Metabolic-Function-are-Altered-by-Resveratrol,139081,0,2.html |
| work_keys_str_mv | AT paulinaszczurekjanicka expressionprofileofbrainagingandmetabolicfunctionarealteredbyresveratroloraketoglutaratesupplementationinratsfedahighfatdiet AT katarzynaropkamolik expressionprofileofbrainagingandmetabolicfunctionarealteredbyresveratroloraketoglutaratesupplementationinratsfedahighfatdiet AT mariaoczkowicz expressionprofileofbrainagingandmetabolicfunctionarealteredbyresveratroloraketoglutaratesupplementationinratsfedahighfatdiet AT sylwiaorczewskadudek expressionprofileofbrainagingandmetabolicfunctionarealteredbyresveratroloraketoglutaratesupplementationinratsfedahighfatdiet AT mariuszpietras expressionprofileofbrainagingandmetabolicfunctionarealteredbyresveratroloraketoglutaratesupplementationinratsfedahighfatdiet AT marekpieszka expressionprofileofbrainagingandmetabolicfunctionarealteredbyresveratroloraketoglutaratesupplementationinratsfedahighfatdiet |