A signal processing analysis of Purkinje cells <i>in vitro</i>
Cerebellar Purkinje cells in vitro fire recurrent sequences of Sodium and Calcium spikes. Here, we analyze the Purkinje cell using harmonic analysis, and our experiments reveal that its output signal is comprised of three distinct frequency bands, which are combined using Amplitude and Frequency Mod...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2010-05-01
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| Series: | Frontiers in Neural Circuits |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncir.2010.00013/full |
| _version_ | 1818531469523419136 |
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| author | Ze'ev R Abrams Ze'ev R Abrams Ajithkumar Warrier Dirk Trauner Xiang Zhang Xiang Zhang |
| author_facet | Ze'ev R Abrams Ze'ev R Abrams Ajithkumar Warrier Dirk Trauner Xiang Zhang Xiang Zhang |
| author_sort | Ze'ev R Abrams |
| collection | DOAJ |
| description | Cerebellar Purkinje cells in vitro fire recurrent sequences of Sodium and Calcium spikes. Here, we analyze the Purkinje cell using harmonic analysis, and our experiments reveal that its output signal is comprised of three distinct frequency bands, which are combined using Amplitude and Frequency Modulation (AM/FM). We find that the three characteristic frequencies - Sodium, Calcium and Switching – occur in various combinations in all waveforms observed using whole-cell current clamp recordings. We found that the Calcium frequency can display a frequency doubling of its frequency mode, and the Switching frequency can act as a possible generator of pauses that are typically seen in Purkinje output recordings. Using a reversibly photo-switchable kainate receptor agonist, we demonstrate the external modulation of the Calcium and Switching frequencies. These experiments and Fourier analysis suggest that the Purkinje cell can be understood as a harmonic signal oscillator, enabling a higher level of interpretation of Purkinje signaling based on modern signal processing techniques. |
| first_indexed | 2024-12-11T17:32:52Z |
| format | Article |
| id | doaj.art-030388427af5487893e97995390f0314 |
| institution | Directory Open Access Journal |
| issn | 1662-5110 |
| language | English |
| last_indexed | 2024-12-11T17:32:52Z |
| publishDate | 2010-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neural Circuits |
| spelling | doaj.art-030388427af5487893e97995390f03142022-12-22T00:56:46ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102010-05-01410.3389/fncir.2010.000131195A signal processing analysis of Purkinje cells <i>in vitro</i>Ze'ev R Abrams0Ze'ev R Abrams1Ajithkumar Warrier2Dirk Trauner3Xiang Zhang4Xiang Zhang5Nanoscale Science and Engineering Center,Helen Wills Neuroscience Institute,University of California, BerkeleyUniversity of California, BerkeleyNanoscale Science and Engineering Center,Helen Wills Neuroscience Institute,University of California, BerkeleyLudwig-Maximilians-Universität MünchenNanoscale Science and Engineering Center,Helen Wills Neuroscience Institute,University of California, BerkeleyUniversity of California, BerkeleyCerebellar Purkinje cells in vitro fire recurrent sequences of Sodium and Calcium spikes. Here, we analyze the Purkinje cell using harmonic analysis, and our experiments reveal that its output signal is comprised of three distinct frequency bands, which are combined using Amplitude and Frequency Modulation (AM/FM). We find that the three characteristic frequencies - Sodium, Calcium and Switching – occur in various combinations in all waveforms observed using whole-cell current clamp recordings. We found that the Calcium frequency can display a frequency doubling of its frequency mode, and the Switching frequency can act as a possible generator of pauses that are typically seen in Purkinje output recordings. Using a reversibly photo-switchable kainate receptor agonist, we demonstrate the external modulation of the Calcium and Switching frequencies. These experiments and Fourier analysis suggest that the Purkinje cell can be understood as a harmonic signal oscillator, enabling a higher level of interpretation of Purkinje signaling based on modern signal processing techniques.http://journal.frontiersin.org/Journal/10.3389/fncir.2010.00013/fullPurkinje celloscillationsSignal processingcalcium spikesoptical activationrhythmicity |
| spellingShingle | Ze'ev R Abrams Ze'ev R Abrams Ajithkumar Warrier Dirk Trauner Xiang Zhang Xiang Zhang A signal processing analysis of Purkinje cells <i>in vitro</i> Frontiers in Neural Circuits Purkinje cell oscillations Signal processing calcium spikes optical activation rhythmicity |
| title | A signal processing analysis of Purkinje cells <i>in vitro</i> |
| title_full | A signal processing analysis of Purkinje cells <i>in vitro</i> |
| title_fullStr | A signal processing analysis of Purkinje cells <i>in vitro</i> |
| title_full_unstemmed | A signal processing analysis of Purkinje cells <i>in vitro</i> |
| title_short | A signal processing analysis of Purkinje cells <i>in vitro</i> |
| title_sort | signal processing analysis of purkinje cells i in vitro i |
| topic | Purkinje cell oscillations Signal processing calcium spikes optical activation rhythmicity |
| url | http://journal.frontiersin.org/Journal/10.3389/fncir.2010.00013/full |
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