Anti-Aging, Anti-Acne, and Cytotoxic Activities of <i>Houttuynia cordata</i> Extracts and Phytochemicals Analysis by LC-MS/MS
Although many biological properties of <i>Houttuynia cordata</i> have been found, its anti-aging and anti-acne effects have not yet been investigated. This study was aimed to evaluate the in vitro anti-aging and anti-acne activities of <i>H. cordata</i> extracts and their cyt...
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MDPI AG
2022-12-01
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| Series: | Cosmetics |
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| Online Access: | https://www.mdpi.com/2079-9284/9/6/136 |
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| author | Santi Phosri Kanokwan Kiattisin Aekkhaluck Intharuksa Raveeporn Janon Tanat Na Nongkhai Tinnakorn Theansungnoen |
| author_facet | Santi Phosri Kanokwan Kiattisin Aekkhaluck Intharuksa Raveeporn Janon Tanat Na Nongkhai Tinnakorn Theansungnoen |
| author_sort | Santi Phosri |
| collection | DOAJ |
| description | Although many biological properties of <i>Houttuynia cordata</i> have been found, its anti-aging and anti-acne effects have not yet been investigated. This study was aimed to evaluate the in vitro anti-aging and anti-acne activities of <i>H. cordata</i> extracts and their cytotoxic activities and phytochemicals analyzed with liquid chromatography with tandem mass spectrometry (LC-MS/MS). Dried aerial parts of <i>H. cordata</i> were given different extractions. The aqueous and ethanolic extracts obtained were named HCA and HCE, respectively, and used to screen total phenolic and flavonoid contents. In vitro anti-aging, skin-related antimicrobial, scanning electron microscopy (SEM), in vitro cytotoxic, and LC-MS/MS analyses were performed. The total phenolic contents of the HCA and HCE were 5.11 ± 0.25 and 27.02 ± 1.07 mg gallic acid equivalent (GAE)/g dry extract while their total flavonoid contents were 104.94 ± 5.16 and 571.86 ± 2.86 mg quercetin equivalent (QE)/g dry extract, respectively. The HCA and HCE inhibited the activities of collagenase (28.33–46.00%), elastase (30.00–34.33%), and hyaluronidase (93.87–98.72%). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the HCA against <i>Cutibacterium acnes</i> DMST14916 were 5.77 and 5.77 mg/mL while those of the HCE were 2.47 and 2.47 mg/mL, respectively. Cell collapses of <i>C. acnes</i> after treatment with the extracts were observed with SEM. The HCE was not toxic to macrophages, keratinocytes, and fibroblasts up to 400 mg/mL. The HCA showed toxicity against macrophages at 62.5 mg/mL and both skin cells at 250 mg/mL. The main phytochemicals in the extracts were identified with LC-MS/MS. Phenolic compounds, flavonoids, and flavonoid derivatives in <i>H. cordata</i> extracts could be major phytochemicals to possess a broad spectrum of biological activities including antioxidant, antimicrobial, and anti-aging activities. The findings from this study showed that the HCE has potential anti-aging and anti-acne properties while having non-cytotoxic activities on the immune and skin cells. These results indicate that the extract is probably advantageous in the development of skincare cosmeceutics and beauty treatments. |
| first_indexed | 2024-03-09T17:09:57Z |
| format | Article |
| id | doaj.art-0304e88ba93446ce81fab494f2ff713b |
| institution | Directory Open Access Journal |
| issn | 2079-9284 |
| language | English |
| last_indexed | 2024-03-09T17:09:57Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
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| series | Cosmetics |
| spelling | doaj.art-0304e88ba93446ce81fab494f2ff713b2023-11-24T14:08:45ZengMDPI AGCosmetics2079-92842022-12-019613610.3390/cosmetics9060136Anti-Aging, Anti-Acne, and Cytotoxic Activities of <i>Houttuynia cordata</i> Extracts and Phytochemicals Analysis by LC-MS/MSSanti Phosri0Kanokwan Kiattisin1Aekkhaluck Intharuksa2Raveeporn Janon3Tanat Na Nongkhai4Tinnakorn Theansungnoen5Department of Chemical Engineering, Faculty of Engineering, Burapha University, Chonburi 20131, ThailandInnovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, ThailandSchool of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, ThailandSchool of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, ThailandAlthough many biological properties of <i>Houttuynia cordata</i> have been found, its anti-aging and anti-acne effects have not yet been investigated. This study was aimed to evaluate the in vitro anti-aging and anti-acne activities of <i>H. cordata</i> extracts and their cytotoxic activities and phytochemicals analyzed with liquid chromatography with tandem mass spectrometry (LC-MS/MS). Dried aerial parts of <i>H. cordata</i> were given different extractions. The aqueous and ethanolic extracts obtained were named HCA and HCE, respectively, and used to screen total phenolic and flavonoid contents. In vitro anti-aging, skin-related antimicrobial, scanning electron microscopy (SEM), in vitro cytotoxic, and LC-MS/MS analyses were performed. The total phenolic contents of the HCA and HCE were 5.11 ± 0.25 and 27.02 ± 1.07 mg gallic acid equivalent (GAE)/g dry extract while their total flavonoid contents were 104.94 ± 5.16 and 571.86 ± 2.86 mg quercetin equivalent (QE)/g dry extract, respectively. The HCA and HCE inhibited the activities of collagenase (28.33–46.00%), elastase (30.00–34.33%), and hyaluronidase (93.87–98.72%). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the HCA against <i>Cutibacterium acnes</i> DMST14916 were 5.77 and 5.77 mg/mL while those of the HCE were 2.47 and 2.47 mg/mL, respectively. Cell collapses of <i>C. acnes</i> after treatment with the extracts were observed with SEM. The HCE was not toxic to macrophages, keratinocytes, and fibroblasts up to 400 mg/mL. The HCA showed toxicity against macrophages at 62.5 mg/mL and both skin cells at 250 mg/mL. The main phytochemicals in the extracts were identified with LC-MS/MS. Phenolic compounds, flavonoids, and flavonoid derivatives in <i>H. cordata</i> extracts could be major phytochemicals to possess a broad spectrum of biological activities including antioxidant, antimicrobial, and anti-aging activities. The findings from this study showed that the HCE has potential anti-aging and anti-acne properties while having non-cytotoxic activities on the immune and skin cells. These results indicate that the extract is probably advantageous in the development of skincare cosmeceutics and beauty treatments.https://www.mdpi.com/2079-9284/9/6/136anti-acneanti-agingchemical analysiscytotoxicity<i>Houttuynia cordata</i>LC-MS/MS |
| spellingShingle | Santi Phosri Kanokwan Kiattisin Aekkhaluck Intharuksa Raveeporn Janon Tanat Na Nongkhai Tinnakorn Theansungnoen Anti-Aging, Anti-Acne, and Cytotoxic Activities of <i>Houttuynia cordata</i> Extracts and Phytochemicals Analysis by LC-MS/MS Cosmetics anti-acne anti-aging chemical analysis cytotoxicity <i>Houttuynia cordata</i> LC-MS/MS |
| title | Anti-Aging, Anti-Acne, and Cytotoxic Activities of <i>Houttuynia cordata</i> Extracts and Phytochemicals Analysis by LC-MS/MS |
| title_full | Anti-Aging, Anti-Acne, and Cytotoxic Activities of <i>Houttuynia cordata</i> Extracts and Phytochemicals Analysis by LC-MS/MS |
| title_fullStr | Anti-Aging, Anti-Acne, and Cytotoxic Activities of <i>Houttuynia cordata</i> Extracts and Phytochemicals Analysis by LC-MS/MS |
| title_full_unstemmed | Anti-Aging, Anti-Acne, and Cytotoxic Activities of <i>Houttuynia cordata</i> Extracts and Phytochemicals Analysis by LC-MS/MS |
| title_short | Anti-Aging, Anti-Acne, and Cytotoxic Activities of <i>Houttuynia cordata</i> Extracts and Phytochemicals Analysis by LC-MS/MS |
| title_sort | anti aging anti acne and cytotoxic activities of i houttuynia cordata i extracts and phytochemicals analysis by lc ms ms |
| topic | anti-acne anti-aging chemical analysis cytotoxicity <i>Houttuynia cordata</i> LC-MS/MS |
| url | https://www.mdpi.com/2079-9284/9/6/136 |
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