Protective Effects of <i>Hippophae rhamnoides</i> L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in Zebrafish
<i>Hippophae rhamnoides</i> L. is a deciduous shrub that contains many unique bioactive substances. This sea buckthorn possesses anticancer, antioxidant, anti-inflammatory, and cardiovascular protective properties. Herein, the effects of phenylpropyl compounds extracted from <i>H....
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8858 |
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| author | Gang Li Ming Chu Yingying Tong Yuexin Liang Shenghui Wang Chengjun Ma Zhenhua Wang Wenna Zhou |
| author_facet | Gang Li Ming Chu Yingying Tong Yuexin Liang Shenghui Wang Chengjun Ma Zhenhua Wang Wenna Zhou |
| author_sort | Gang Li |
| collection | DOAJ |
| description | <i>Hippophae rhamnoides</i> L. is a deciduous shrub that contains many unique bioactive substances. This sea buckthorn possesses anticancer, antioxidant, anti-inflammatory, and cardiovascular protective properties. Herein, the effects of phenylpropyl compounds extracted from <i>H. rhamnoides</i> L. on doxorubicin (Dox)-induced cardiotoxicity were evaluated in zebrafish. Cardiac injury in zebrafish was induced using 35 μM Dox for 96 h, and 30 μM phenylpropanoid compounds were used as the protective treatment. The cardioprotective effects and mechanisms of the four phenylpropanoids were investigated using microscopy, behavioral analysis, acridine orange staining, western blotting, flow cytometry, and real-time quantitative polymerase chain reaction. The extracted phenylpropanoids could significantly relieve Dox-induced cardiac injury in zebrafish and inhibit cardiomyocyte apoptosis. The mechanisms of action were mainly related to the stability of mitochondrial biogenesis and function maintained by phenylpropanoids in zebrafish. To our knowledge, this is the first report on the protective effect of sea buckthorn against myocardial injury in zebrafish. Our findings provide support for the further research and development of sea buckthorn and its components. |
| first_indexed | 2024-03-09T16:01:52Z |
| format | Article |
| id | doaj.art-030c083329014b89b7da3c1f0674e4e8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:01:52Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-030c083329014b89b7da3c1f0674e4e82023-11-24T16:58:38ZengMDPI AGMolecules1420-30492022-12-012724885810.3390/molecules27248858Protective Effects of <i>Hippophae rhamnoides</i> L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in ZebrafishGang Li0Ming Chu1Yingying Tong2Yuexin Liang3Shenghui Wang4Chengjun Ma5Zhenhua Wang6Wenna Zhou7Center for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai 264005, ChinaCenter for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai 264005, ChinaCenter for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai 264005, ChinaCenter for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai 264005, ChinaDepartment of Pharmaceutical Engineering, School of Life and Health Sciences, Huzhou College, Huzhou 313000, ChinaCenter for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai 264005, ChinaCenter for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai 264005, ChinaDepartment of Pharmaceutical Engineering, School of Life and Health Sciences, Huzhou College, Huzhou 313000, China<i>Hippophae rhamnoides</i> L. is a deciduous shrub that contains many unique bioactive substances. This sea buckthorn possesses anticancer, antioxidant, anti-inflammatory, and cardiovascular protective properties. Herein, the effects of phenylpropyl compounds extracted from <i>H. rhamnoides</i> L. on doxorubicin (Dox)-induced cardiotoxicity were evaluated in zebrafish. Cardiac injury in zebrafish was induced using 35 μM Dox for 96 h, and 30 μM phenylpropanoid compounds were used as the protective treatment. The cardioprotective effects and mechanisms of the four phenylpropanoids were investigated using microscopy, behavioral analysis, acridine orange staining, western blotting, flow cytometry, and real-time quantitative polymerase chain reaction. The extracted phenylpropanoids could significantly relieve Dox-induced cardiac injury in zebrafish and inhibit cardiomyocyte apoptosis. The mechanisms of action were mainly related to the stability of mitochondrial biogenesis and function maintained by phenylpropanoids in zebrafish. To our knowledge, this is the first report on the protective effect of sea buckthorn against myocardial injury in zebrafish. Our findings provide support for the further research and development of sea buckthorn and its components.https://www.mdpi.com/1420-3049/27/24/8858sea buckthornzebrafishdoxorubicincardiotoxicitymitochondria |
| spellingShingle | Gang Li Ming Chu Yingying Tong Yuexin Liang Shenghui Wang Chengjun Ma Zhenhua Wang Wenna Zhou Protective Effects of <i>Hippophae rhamnoides</i> L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in Zebrafish Molecules sea buckthorn zebrafish doxorubicin cardiotoxicity mitochondria |
| title | Protective Effects of <i>Hippophae rhamnoides</i> L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in Zebrafish |
| title_full | Protective Effects of <i>Hippophae rhamnoides</i> L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in Zebrafish |
| title_fullStr | Protective Effects of <i>Hippophae rhamnoides</i> L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in Zebrafish |
| title_full_unstemmed | Protective Effects of <i>Hippophae rhamnoides</i> L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in Zebrafish |
| title_short | Protective Effects of <i>Hippophae rhamnoides</i> L. Phenylpropanoids on Doxorubicin-Induced Cardiotoxicity in Zebrafish |
| title_sort | protective effects of i hippophae rhamnoides i l phenylpropanoids on doxorubicin induced cardiotoxicity in zebrafish |
| topic | sea buckthorn zebrafish doxorubicin cardiotoxicity mitochondria |
| url | https://www.mdpi.com/1420-3049/27/24/8858 |
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