Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons
Proneural proteins of the class I/II basic-helix-loop-helix (bHLH) family are highly conserved transcription factors. Class I bHLH proteins are expressed in a broad number of tissues during development, whereas class II bHLH protein expression is more tissue restricted. Our understanding of the func...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-04-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112471630290X |
| _version_ | 1818022741713879040 |
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| author | Mitchell D’Rozario Ting Zhang Edward A. Waddell Yonggang Zhang Cem Sahin Michal Sharoni Tina Hu Mohammad Nayal Kaveesh Kutty Faith Liebl Wenhui Hu Daniel R. Marenda |
| author_facet | Mitchell D’Rozario Ting Zhang Edward A. Waddell Yonggang Zhang Cem Sahin Michal Sharoni Tina Hu Mohammad Nayal Kaveesh Kutty Faith Liebl Wenhui Hu Daniel R. Marenda |
| author_sort | Mitchell D’Rozario |
| collection | DOAJ |
| description | Proneural proteins of the class I/II basic-helix-loop-helix (bHLH) family are highly conserved transcription factors. Class I bHLH proteins are expressed in a broad number of tissues during development, whereas class II bHLH protein expression is more tissue restricted. Our understanding of the function of class I/II bHLH transcription factors in both invertebrate and vertebrate neurobiology is largely focused on their function as regulators of neurogenesis. Here, we show that the class I bHLH proteins Daughterless and Tcf4 are expressed in postmitotic neurons in Drosophila melanogaster and mice, respectively, where they function to restrict neurite branching and synapse formation. Our data indicate that Daughterless performs this function in part by restricting the expression of the cell adhesion molecule Neurexin. This suggests a role for these proteins outside of their established roles in neurogenesis. |
| first_indexed | 2024-12-10T03:33:15Z |
| format | Article |
| id | doaj.art-030c7014471548abaa2e6ab34c59e9db |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-10T03:33:15Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-030c7014471548abaa2e6ab34c59e9db2022-12-22T02:03:45ZengElsevierCell Reports2211-12472016-04-0115238639710.1016/j.celrep.2016.03.034Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic NeuronsMitchell D’Rozario0Ting Zhang1Edward A. Waddell2Yonggang Zhang3Cem Sahin4Michal Sharoni5Tina Hu6Mohammad Nayal7Kaveesh Kutty8Faith Liebl9Wenhui Hu10Daniel R. Marenda11Department of Biology, Drexel University, Philadelphia, PA 19104, USADepartment of Neuroscience, Temple University School of Medicine, Philadelphia, PA 19140, USADepartment of Biology, Drexel University, Philadelphia, PA 19104, USADepartment of Neuroscience, Temple University School of Medicine, Philadelphia, PA 19140, USADepartment of Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USADepartment of Biology, Drexel University, Philadelphia, PA 19104, USADepartment of Biology, Drexel University, Philadelphia, PA 19104, USADepartment of Biology, Drexel University, Philadelphia, PA 19104, USADepartment of Biology, Drexel University, Philadelphia, PA 19104, USADepartment of Biological Sciences, Southern Illinois University Edwardsville, Edwardsville, IL 62026, USADepartment of Neuroscience, Temple University School of Medicine, Philadelphia, PA 19140, USADepartment of Biology, Drexel University, Philadelphia, PA 19104, USAProneural proteins of the class I/II basic-helix-loop-helix (bHLH) family are highly conserved transcription factors. Class I bHLH proteins are expressed in a broad number of tissues during development, whereas class II bHLH protein expression is more tissue restricted. Our understanding of the function of class I/II bHLH transcription factors in both invertebrate and vertebrate neurobiology is largely focused on their function as regulators of neurogenesis. Here, we show that the class I bHLH proteins Daughterless and Tcf4 are expressed in postmitotic neurons in Drosophila melanogaster and mice, respectively, where they function to restrict neurite branching and synapse formation. Our data indicate that Daughterless performs this function in part by restricting the expression of the cell adhesion molecule Neurexin. This suggests a role for these proteins outside of their established roles in neurogenesis.http://www.sciencedirect.com/science/article/pii/S221112471630290XdaughterlessTCF4NMJproneuralbHLHPitt-Hopkinsschizophrenia |
| spellingShingle | Mitchell D’Rozario Ting Zhang Edward A. Waddell Yonggang Zhang Cem Sahin Michal Sharoni Tina Hu Mohammad Nayal Kaveesh Kutty Faith Liebl Wenhui Hu Daniel R. Marenda Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons Cell Reports daughterless TCF4 NMJ proneural bHLH Pitt-Hopkins schizophrenia |
| title | Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons |
| title_full | Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons |
| title_fullStr | Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons |
| title_full_unstemmed | Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons |
| title_short | Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons |
| title_sort | type i bhlh proteins daughterless and tcf4 restrict neurite branching and synapse formation by repressing neurexin in postmitotic neurons |
| topic | daughterless TCF4 NMJ proneural bHLH Pitt-Hopkins schizophrenia |
| url | http://www.sciencedirect.com/science/article/pii/S221112471630290X |
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