Effects of different levels of rutin on growth performance, immunity, intestinal barrier and antioxidant capacity of broilers
This study investigated the effects of different doses of rutin on the growth performance, immunity, intestinal barrier, and antioxidant capacity of broilers. A total of two hundred and fifty-six 1-day-old male broilers were divided into 4 groups and fed basal diets supplemented with 0 (control grou...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1828051X.2022.2116732 |
| _version_ | 1797737059256893440 |
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| author | Shun Chen Huijuan Liu Jiaqi Zhang Binbin Zhou Su Zhuang Xiaofang He Tian Wang Chao Wang |
| author_facet | Shun Chen Huijuan Liu Jiaqi Zhang Binbin Zhou Su Zhuang Xiaofang He Tian Wang Chao Wang |
| author_sort | Shun Chen |
| collection | DOAJ |
| description | This study investigated the effects of different doses of rutin on the growth performance, immunity, intestinal barrier, and antioxidant capacity of broilers. A total of two hundred and fifty-six 1-day-old male broilers were divided into 4 groups and fed basal diets supplemented with 0 (control group), 250, 500 and 1,000 mg rutin/kg, respectively, for 42 days. In the starter period (days 1–21) and whole trial period (days 1–42), broilers fed diets containing 500 mg rutin/kg had significantly (p < 0.05) higher average daily feed intake, body weight and average daily gain and better feed conversion ratio. Dietary 500 mg rutin/kg increased the villus height, villus height to crypt depth ratio and villus area, while reducing the B cell lymphoma 2 associated X mRNA expression (p < 0.05). Dietary 500 mg rutin/kg increased the level of IgA in serum and decreased serum tumour necrosis factor-α (TNF-α) content, and the nuclear factor kappa-B, interleukin-2 and TNF-α mRNA expression in jejunal mucosa (p < 0.05). Simultaneously, 500 and 1,000 mg rutin/kg significantly decreased serum diamine oxidase activity, D-lactic acid and lipopolysaccharide concentration, increased activities of total superoxide dismutase and total antioxidant capacity in jejunal mucosa (p < 0.05), and upregulated mRNA expressions of genes related to intestinal barrier and antioxidant capacity in jejunal mucosa (p < 0.05). These findings indicate that dietary rutin, especially at 500 mg/kg, improves broilers’ growth performance, intestinal barrier function, immunity, and antioxidant capability, which may be related to the nuclear factor erythroid-2-related factor 2 (Nrf2) pathway.Highlights Dietary rutin improved growth performance, jejunal morphology, and intestinal barrier function. Dietary rutin enhanced the immunity via inhibiting NF-κB, and improved antioxidant capacity via activating Nrf2/HO-1 pathway in broilers. The optimal dose of rutin was 500 mg/kg in broiler diet. |
| first_indexed | 2024-03-12T13:21:57Z |
| format | Article |
| id | doaj.art-031aecd6ef8c4812bd41005949c368d7 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-03-12T13:21:57Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-031aecd6ef8c4812bd41005949c368d72023-08-25T13:51:05ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2022-12-012111390140110.1080/1828051X.2022.21167322116732Effects of different levels of rutin on growth performance, immunity, intestinal barrier and antioxidant capacity of broilersShun Chen0Huijuan Liu1Jiaqi Zhang2Binbin Zhou3Su Zhuang4Xiaofang He5Tian Wang6Chao Wang7College of Animal Science and Technology, National Experimental Teaching Demonstration Centre of Animal Science, Nanjing Agricultural UniversityCollege of Animal Science and Technology, National Experimental Teaching Demonstration Centre of Animal Science, Nanjing Agricultural UniversityCollege of Animal Science and Technology, National Experimental Teaching Demonstration Centre of Animal Science, Nanjing Agricultural UniversityCollege of Animal Science and Technology, National Experimental Teaching Demonstration Centre of Animal Science, Nanjing Agricultural UniversityCollege of Animal Science and Technology, National Experimental Teaching Demonstration Centre of Animal Science, Nanjing Agricultural UniversityCo-Innovation Center for School of Animal Science and Food Engineering, Jinling Institute of TechnologyCollege of Animal Science and Technology, National Experimental Teaching Demonstration Centre of Animal Science, Nanjing Agricultural UniversityCollege of Animal Science and Technology, National Experimental Teaching Demonstration Centre of Animal Science, Nanjing Agricultural UniversityThis study investigated the effects of different doses of rutin on the growth performance, immunity, intestinal barrier, and antioxidant capacity of broilers. A total of two hundred and fifty-six 1-day-old male broilers were divided into 4 groups and fed basal diets supplemented with 0 (control group), 250, 500 and 1,000 mg rutin/kg, respectively, for 42 days. In the starter period (days 1–21) and whole trial period (days 1–42), broilers fed diets containing 500 mg rutin/kg had significantly (p < 0.05) higher average daily feed intake, body weight and average daily gain and better feed conversion ratio. Dietary 500 mg rutin/kg increased the villus height, villus height to crypt depth ratio and villus area, while reducing the B cell lymphoma 2 associated X mRNA expression (p < 0.05). Dietary 500 mg rutin/kg increased the level of IgA in serum and decreased serum tumour necrosis factor-α (TNF-α) content, and the nuclear factor kappa-B, interleukin-2 and TNF-α mRNA expression in jejunal mucosa (p < 0.05). Simultaneously, 500 and 1,000 mg rutin/kg significantly decreased serum diamine oxidase activity, D-lactic acid and lipopolysaccharide concentration, increased activities of total superoxide dismutase and total antioxidant capacity in jejunal mucosa (p < 0.05), and upregulated mRNA expressions of genes related to intestinal barrier and antioxidant capacity in jejunal mucosa (p < 0.05). These findings indicate that dietary rutin, especially at 500 mg/kg, improves broilers’ growth performance, intestinal barrier function, immunity, and antioxidant capability, which may be related to the nuclear factor erythroid-2-related factor 2 (Nrf2) pathway.Highlights Dietary rutin improved growth performance, jejunal morphology, and intestinal barrier function. Dietary rutin enhanced the immunity via inhibiting NF-κB, and improved antioxidant capacity via activating Nrf2/HO-1 pathway in broilers. The optimal dose of rutin was 500 mg/kg in broiler diet.http://dx.doi.org/10.1080/1828051X.2022.2116732rutinimmune systemantioxidant statusintestinal barrier functionbroiler |
| spellingShingle | Shun Chen Huijuan Liu Jiaqi Zhang Binbin Zhou Su Zhuang Xiaofang He Tian Wang Chao Wang Effects of different levels of rutin on growth performance, immunity, intestinal barrier and antioxidant capacity of broilers Italian Journal of Animal Science rutin immune system antioxidant status intestinal barrier function broiler |
| title | Effects of different levels of rutin on growth performance, immunity, intestinal barrier and antioxidant capacity of broilers |
| title_full | Effects of different levels of rutin on growth performance, immunity, intestinal barrier and antioxidant capacity of broilers |
| title_fullStr | Effects of different levels of rutin on growth performance, immunity, intestinal barrier and antioxidant capacity of broilers |
| title_full_unstemmed | Effects of different levels of rutin on growth performance, immunity, intestinal barrier and antioxidant capacity of broilers |
| title_short | Effects of different levels of rutin on growth performance, immunity, intestinal barrier and antioxidant capacity of broilers |
| title_sort | effects of different levels of rutin on growth performance immunity intestinal barrier and antioxidant capacity of broilers |
| topic | rutin immune system antioxidant status intestinal barrier function broiler |
| url | http://dx.doi.org/10.1080/1828051X.2022.2116732 |
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