Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods
Evaluation of sperm morphometry is an important criterion in the diagnosis of a male animal’s suitability for breeding. The aim of the study was to evaluate the morphometry of sperm from the epididymides of dogs subjected to routine castration using various staining methods. The study was carried ou...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/1/227 |
| _version_ | 1797410131253657600 |
|---|---|
| author | Anna Wysokińska Ewa Wójcik Angelika Chłopik |
| author_facet | Anna Wysokińska Ewa Wójcik Angelika Chłopik |
| author_sort | Anna Wysokińska |
| collection | DOAJ |
| description | Evaluation of sperm morphometry is an important criterion in the diagnosis of a male animal’s suitability for breeding. The aim of the study was to evaluate the morphometry of sperm from the epididymides of dogs subjected to routine castration using various staining methods. The study was carried out on semen collected from ten healthy dogs. Gonads were obtained from each dog during routine castration at a veterinary surgery. Then, the epididymides (caput, corpus, cauda) were isolated from the gonads, semen was collected from them and microscope slides were prepared. The slides for evaluation of sperm morphometry were prepared by four methods: DiffQuik, SpermBlue, eosin-nigrosin and eosin-gentian. A total of 2400 sperm were analyzed (240 sperm from the dog). The sperm collected from the caput and corpus of the epididymis were found to have larger heads and tails than those collected from the cauda of the epididymis. The staining method was shown to affect the morphometry of sperm taken from the epididymides of dogs. The staining methods differentiate the dimensions of the head of sperm in different parts of the epididymis but do not affect the length of the sperm tail. The occurrence of differences in the head dimensions of sperm may be linked to the use of different fixatives and chemical reagents in the staining procedure. Sperm stained by the EN method had the smallest head and tail dimensions. The greatest head area was noted in the sperm stained by the EG method. In the slides stained by the SB method, the sperm heads were relatively long but narrow. The methods used are suitable for the evaluation of sperm structure, and the possibility of using all four methods enables a full characterization of sperm collected from the caput, corpus and cauda of the epididymides of dogs. |
| first_indexed | 2024-03-09T04:24:28Z |
| format | Article |
| id | doaj.art-031d64b5635e40fa98de730dbf4bf0bd |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T04:24:28Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-031d64b5635e40fa98de730dbf4bf0bd2023-12-03T13:41:45ZengMDPI AGAnimals2076-26152021-01-0111122710.3390/ani11010227Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining MethodsAnna Wysokińska0Ewa Wójcik1Angelika Chłopik2Faculty of Agrobioengineering and Animal Husbandry, Siedlce University of Natural Sciences and Humanities, 08110 Siedlce, PolandFaculty of Agrobioengineering and Animal Husbandry, Siedlce University of Natural Sciences and Humanities, 08110 Siedlce, PolandFaculty of Agrobioengineering and Animal Husbandry, Siedlce University of Natural Sciences and Humanities, 08110 Siedlce, PolandEvaluation of sperm morphometry is an important criterion in the diagnosis of a male animal’s suitability for breeding. The aim of the study was to evaluate the morphometry of sperm from the epididymides of dogs subjected to routine castration using various staining methods. The study was carried out on semen collected from ten healthy dogs. Gonads were obtained from each dog during routine castration at a veterinary surgery. Then, the epididymides (caput, corpus, cauda) were isolated from the gonads, semen was collected from them and microscope slides were prepared. The slides for evaluation of sperm morphometry were prepared by four methods: DiffQuik, SpermBlue, eosin-nigrosin and eosin-gentian. A total of 2400 sperm were analyzed (240 sperm from the dog). The sperm collected from the caput and corpus of the epididymis were found to have larger heads and tails than those collected from the cauda of the epididymis. The staining method was shown to affect the morphometry of sperm taken from the epididymides of dogs. The staining methods differentiate the dimensions of the head of sperm in different parts of the epididymis but do not affect the length of the sperm tail. The occurrence of differences in the head dimensions of sperm may be linked to the use of different fixatives and chemical reagents in the staining procedure. Sperm stained by the EN method had the smallest head and tail dimensions. The greatest head area was noted in the sperm stained by the EG method. In the slides stained by the SB method, the sperm heads were relatively long but narrow. The methods used are suitable for the evaluation of sperm structure, and the possibility of using all four methods enables a full characterization of sperm collected from the caput, corpus and cauda of the epididymides of dogs.https://www.mdpi.com/2076-2615/11/1/227canine spermatozoaepididymissperm morphometrystaining methods |
| spellingShingle | Anna Wysokińska Ewa Wójcik Angelika Chłopik Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods Animals canine spermatozoa epididymis sperm morphometry staining methods |
| title | Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods |
| title_full | Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods |
| title_fullStr | Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods |
| title_full_unstemmed | Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods |
| title_short | Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods |
| title_sort | evaluation of the morphometry of sperm from the epididymides of dogs using different staining methods |
| topic | canine spermatozoa epididymis sperm morphometry staining methods |
| url | https://www.mdpi.com/2076-2615/11/1/227 |
| work_keys_str_mv | AT annawysokinska evaluationofthemorphometryofspermfromtheepididymidesofdogsusingdifferentstainingmethods AT ewawojcik evaluationofthemorphometryofspermfromtheepididymidesofdogsusingdifferentstainingmethods AT angelikachłopik evaluationofthemorphometryofspermfromtheepididymidesofdogsusingdifferentstainingmethods |