Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria
Some soil bacteria are able to metabolize styrene via initial side-chain oxygenation. This catabolic route is of potential biotechnological relevance due to the occurrence of phenylacetic acid as a central metabolite. The styrene-degrading strains Rhodococcus opacus 1CP, Pseudomonas fluorescens ST,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X15000065 |
| _version_ | 1819206280195080192 |
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| author | Michel Oelschlägel Stefan R. Kaschabek Juliane Zimmerling Michael Schlömann Dirk Tischler |
| author_facet | Michel Oelschlägel Stefan R. Kaschabek Juliane Zimmerling Michael Schlömann Dirk Tischler |
| author_sort | Michel Oelschlägel |
| collection | DOAJ |
| description | Some soil bacteria are able to metabolize styrene via initial side-chain oxygenation. This catabolic route is of potential biotechnological relevance due to the occurrence of phenylacetic acid as a central metabolite.
The styrene-degrading strains Rhodococcus opacus 1CP, Pseudomonas fluorescens ST, and the novel isolates Sphingopyxis sp. Kp5.2 and Gordonia sp. CWB2 were investigated with respect to their applicability to co-metabolically produce substituted phenylacetic acids. Isolates were found to differ significantly in substrate tolerance and biotransformation yields. Especially, P. fluorescens ST was identified as a promising candidate for the production of several phenylacetic acids. The biotransformation of 4-chlorostyrene with cells of strain ST was shown to be stable over a period of more than 200 days and yielded about 38 mmolproduct gcelldryweight−1 after nearly 350 days. Moreover, 4-chloro-α-methylstyrene was predominantly converted to the (S)-enantiomer of the acid with 40% enantiomeric excess. |
| first_indexed | 2024-12-23T05:05:05Z |
| format | Article |
| id | doaj.art-031e3aec5a814a80b8dfe1de42928e50 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-23T05:05:05Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-031e3aec5a814a80b8dfe1de42928e502022-12-21T17:59:06ZengElsevierBiotechnology Reports2215-017X2015-06-016C202610.1016/j.btre.2015.01.003Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteriaMichel OelschlägelStefan R. KaschabekJuliane ZimmerlingMichael SchlömannDirk TischlerSome soil bacteria are able to metabolize styrene via initial side-chain oxygenation. This catabolic route is of potential biotechnological relevance due to the occurrence of phenylacetic acid as a central metabolite. The styrene-degrading strains Rhodococcus opacus 1CP, Pseudomonas fluorescens ST, and the novel isolates Sphingopyxis sp. Kp5.2 and Gordonia sp. CWB2 were investigated with respect to their applicability to co-metabolically produce substituted phenylacetic acids. Isolates were found to differ significantly in substrate tolerance and biotransformation yields. Especially, P. fluorescens ST was identified as a promising candidate for the production of several phenylacetic acids. The biotransformation of 4-chlorostyrene with cells of strain ST was shown to be stable over a period of more than 200 days and yielded about 38 mmolproduct gcelldryweight−1 after nearly 350 days. Moreover, 4-chloro-α-methylstyrene was predominantly converted to the (S)-enantiomer of the acid with 40% enantiomeric excess.http://www.sciencedirect.com/science/article/pii/S2215017X15000065IbuprofenBiocatalysisStyrene degradation |
| spellingShingle | Michel Oelschlägel Stefan R. Kaschabek Juliane Zimmerling Michael Schlömann Dirk Tischler Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria Biotechnology Reports Ibuprofen Biocatalysis Styrene degradation |
| title | Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria |
| title_full | Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria |
| title_fullStr | Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria |
| title_full_unstemmed | Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria |
| title_short | Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria |
| title_sort | co metabolic formation of substituted phenylacetic acids by styrene degrading bacteria |
| topic | Ibuprofen Biocatalysis Styrene degradation |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X15000065 |
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