Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection
A global lipidomic analysis using liquid chromatography–tandem mass spectrometry was performed on the liver of beagle dogs infected with Toxocara canis to profile hepatic lipid species at 12 h post-infection (hpi), 24 hpi, and 36 days post-infection (dpi). This analysis identified six categories and...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.890589/full |
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| author | Hao-Yu Li Yang Zou Hany M. Elsheikha Yue Xu Lang Cai Shi-Chen Xie Xing-Quan Zhu Xing-Quan Zhu Wen-Bin Zheng |
| author_facet | Hao-Yu Li Yang Zou Hany M. Elsheikha Yue Xu Lang Cai Shi-Chen Xie Xing-Quan Zhu Xing-Quan Zhu Wen-Bin Zheng |
| author_sort | Hao-Yu Li |
| collection | DOAJ |
| description | A global lipidomic analysis using liquid chromatography–tandem mass spectrometry was performed on the liver of beagle dogs infected with Toxocara canis to profile hepatic lipid species at 12 h post-infection (hpi), 24 hpi, and 36 days post-infection (dpi). This analysis identified six categories and 42 subclasses of lipids, including 173, 64, and 116 differentially abundant lipid species at 12 hpi, 24 hpi, and 36 dpi, respectively. Many of the identified lysophospholipids, such as lysophosphatidylglycerol, lysophosphatidylserine, and lysophosphatidylcholine, may contribute to the migration and development of T. canis during the early infection stage. Pathway analysis revealed significant alterations of several immune-inflammatory pathways, such as the B-cell receptor signaling pathway, the NF-kappa B signaling pathway, and the C-type lectin receptor signaling pathway at 12 and 24 hpi. These findings demonstrate the value of lipidomic profiling in revealing the extent of changes in the composition and abundance of hepatic lipidome caused by T. canis infection and their relevance to the pathophysiology of toxocariasis in beagle dogs. |
| first_indexed | 2024-04-11T21:07:24Z |
| format | Article |
| id | doaj.art-031f570b70b849f9ae46ee32b705718f |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T21:07:24Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-031f570b70b849f9ae46ee32b705718f2022-12-22T04:03:11ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-09-011210.3389/fcimb.2022.890589890589Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infectionHao-Yu Li0Yang Zou1Hany M. Elsheikha2Yue Xu3Lang Cai4Shi-Chen Xie5Xing-Quan Zhu6Xing-Quan Zhu7Wen-Bin Zheng8Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaFaculty of Medicine and Health Sciences, School of Veterinary Medicine and Science, University of Nottingham, Loughborough, United KingdomLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, ChinaKey Laboratory of Veterinary Public Health of Higher Education of Yunnan Province, College of Veterinary Medicine, Yunnan Agricultural University, Kunming, ChinaLaboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, ChinaA global lipidomic analysis using liquid chromatography–tandem mass spectrometry was performed on the liver of beagle dogs infected with Toxocara canis to profile hepatic lipid species at 12 h post-infection (hpi), 24 hpi, and 36 days post-infection (dpi). This analysis identified six categories and 42 subclasses of lipids, including 173, 64, and 116 differentially abundant lipid species at 12 hpi, 24 hpi, and 36 dpi, respectively. Many of the identified lysophospholipids, such as lysophosphatidylglycerol, lysophosphatidylserine, and lysophosphatidylcholine, may contribute to the migration and development of T. canis during the early infection stage. Pathway analysis revealed significant alterations of several immune-inflammatory pathways, such as the B-cell receptor signaling pathway, the NF-kappa B signaling pathway, and the C-type lectin receptor signaling pathway at 12 and 24 hpi. These findings demonstrate the value of lipidomic profiling in revealing the extent of changes in the composition and abundance of hepatic lipidome caused by T. canis infection and their relevance to the pathophysiology of toxocariasis in beagle dogs.https://www.frontiersin.org/articles/10.3389/fcimb.2022.890589/fullToxocara canistoxocariasisbeagle dogliverlipidomics |
| spellingShingle | Hao-Yu Li Yang Zou Hany M. Elsheikha Yue Xu Lang Cai Shi-Chen Xie Xing-Quan Zhu Xing-Quan Zhu Wen-Bin Zheng Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection Frontiers in Cellular and Infection Microbiology Toxocara canis toxocariasis beagle dog liver lipidomics |
| title | Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection |
| title_full | Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection |
| title_fullStr | Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection |
| title_full_unstemmed | Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection |
| title_short | Lipidomic changes in the liver of beagle dogs associated with Toxocara canis infection |
| title_sort | lipidomic changes in the liver of beagle dogs associated with toxocara canis infection |
| topic | Toxocara canis toxocariasis beagle dog liver lipidomics |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.890589/full |
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