Non-invasive quantification of collagen turnover in renal transplant recipients.
Kidney allograft failure due to chronic injury/rejection remains the main cause of graft loss in renal transplant recipients (RTR). Here, we investigated whether specific biomarkers of extracellular matrix (ECM) turnover are associated with allograft function and chronic kidney disease (CKD) stage i...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5400243?pdf=render |
| _version_ | 1818059576050712576 |
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| author | Elisabeth G D Stribos Signe Holm Nielsen Susanne Brix Morten Asser Karsdal Marc A Seelen Harry van Goor Stephan J L Bakker Peter Olinga Henricus A M Mutsaers Federica Genovese |
| author_facet | Elisabeth G D Stribos Signe Holm Nielsen Susanne Brix Morten Asser Karsdal Marc A Seelen Harry van Goor Stephan J L Bakker Peter Olinga Henricus A M Mutsaers Federica Genovese |
| author_sort | Elisabeth G D Stribos |
| collection | DOAJ |
| description | Kidney allograft failure due to chronic injury/rejection remains the main cause of graft loss in renal transplant recipients (RTR). Here, we investigated whether specific biomarkers of extracellular matrix (ECM) turnover are associated with allograft function and chronic kidney disease (CKD) stage in RTR. Seventy-eight patients who attended the University Medical Center Groningen for a routine check-up after kidney transplantation were enrolled in the study. Plasma and/or 24h-urine samples were collected and specific matrix-metalloproteinase-generated neo-epitope fragments of collagens were measured by enzyme-linked immunosorbent assay. Our results demonstrated that urinary levels of C3M, a marker for collagen type III degradation, correlated with estimated glomerular filtration rate (eGFR; r = 0.58, p<0.0001), with lower levels detected in the urine of patients with advanced CKD. In addition, plasma levels of Pro-C6, a marker for collagen type VI formation, significantly increased with disease progression and correlated with eGFR (r = -0.72, p<0.0001). Conversely, plasma C3M and urinary Pro-C6 levels showed no correlation with renal function. We identified two neo-epitope biomarkers of tissue turnover associated with ECM remodeling and fibrosis that can stratify patients by CKD stage. This is as promising first step towards non-invasive monitoring of ECM turnover in the kidneys. |
| first_indexed | 2024-12-10T13:18:43Z |
| format | Article |
| id | doaj.art-03211738a80d450eafdeb991ac44b5f2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T13:18:43Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-03211738a80d450eafdeb991ac44b5f22022-12-22T01:47:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017589810.1371/journal.pone.0175898Non-invasive quantification of collagen turnover in renal transplant recipients.Elisabeth G D StribosSigne Holm NielsenSusanne BrixMorten Asser KarsdalMarc A SeelenHarry van GoorStephan J L BakkerPeter OlingaHenricus A M MutsaersFederica GenoveseKidney allograft failure due to chronic injury/rejection remains the main cause of graft loss in renal transplant recipients (RTR). Here, we investigated whether specific biomarkers of extracellular matrix (ECM) turnover are associated with allograft function and chronic kidney disease (CKD) stage in RTR. Seventy-eight patients who attended the University Medical Center Groningen for a routine check-up after kidney transplantation were enrolled in the study. Plasma and/or 24h-urine samples were collected and specific matrix-metalloproteinase-generated neo-epitope fragments of collagens were measured by enzyme-linked immunosorbent assay. Our results demonstrated that urinary levels of C3M, a marker for collagen type III degradation, correlated with estimated glomerular filtration rate (eGFR; r = 0.58, p<0.0001), with lower levels detected in the urine of patients with advanced CKD. In addition, plasma levels of Pro-C6, a marker for collagen type VI formation, significantly increased with disease progression and correlated with eGFR (r = -0.72, p<0.0001). Conversely, plasma C3M and urinary Pro-C6 levels showed no correlation with renal function. We identified two neo-epitope biomarkers of tissue turnover associated with ECM remodeling and fibrosis that can stratify patients by CKD stage. This is as promising first step towards non-invasive monitoring of ECM turnover in the kidneys.http://europepmc.org/articles/PMC5400243?pdf=render |
| spellingShingle | Elisabeth G D Stribos Signe Holm Nielsen Susanne Brix Morten Asser Karsdal Marc A Seelen Harry van Goor Stephan J L Bakker Peter Olinga Henricus A M Mutsaers Federica Genovese Non-invasive quantification of collagen turnover in renal transplant recipients. PLoS ONE |
| title | Non-invasive quantification of collagen turnover in renal transplant recipients. |
| title_full | Non-invasive quantification of collagen turnover in renal transplant recipients. |
| title_fullStr | Non-invasive quantification of collagen turnover in renal transplant recipients. |
| title_full_unstemmed | Non-invasive quantification of collagen turnover in renal transplant recipients. |
| title_short | Non-invasive quantification of collagen turnover in renal transplant recipients. |
| title_sort | non invasive quantification of collagen turnover in renal transplant recipients |
| url | http://europepmc.org/articles/PMC5400243?pdf=render |
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