Time-of-Day and Age Impact on Memory in Elevated Plus-Maze Test in Rats
The purpose of the present study was to establish the effect of daytime and aging on memory in rats in the Elevated Plus-Maze (EPM) test. Young (2-months) and aged (18-months) male Wistar rats were exposed to the EPM test, at the beginning, mid-time or at the end of the light period. On the acquisit...
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| Format: | Article |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnbeh.2018.00304/full |
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| author | Nicanor Morales-Delgado Nicanor Morales-Delgado Natalija Popović Natalija Popović Ernesto De la Cruz-Sánchez María Caballero Bleda María Caballero Bleda Miroljub Popović Miroljub Popović |
| author_facet | Nicanor Morales-Delgado Nicanor Morales-Delgado Natalija Popović Natalija Popović Ernesto De la Cruz-Sánchez María Caballero Bleda María Caballero Bleda Miroljub Popović Miroljub Popović |
| author_sort | Nicanor Morales-Delgado |
| collection | DOAJ |
| description | The purpose of the present study was to establish the effect of daytime and aging on memory in rats in the Elevated Plus-Maze (EPM) test. Young (2-months) and aged (18-months) male Wistar rats were exposed to the EPM test, at the beginning, mid-time or at the end of the light period. On the acquisition trial, the animals were placed individually at the end of one of the open arms of the EPM and the latency to enter in the enclosed arms was registered (cut-off time 60 s). The test was repeated 24 h later on. A longer latency period to reach the enclosed arm indicated poor retention compared to significantly shorter latencies. There were no significant differences between groups on the acquisition trial. In all tested periods, the latency time on the 24 h retention trial was significantly shorter in the young rats compared to the old ones. Furthermore, in the early and mid-time period of the light period, the young rats showed significantly decreased transfer latency (TL) time on the 24 h retention trial in comparison with the acquisition trial. In the aged rats, the TL time on the 24 h retention trial was significantly longer at the end of the light period, in comparison to the two other testing periods. In conclusion, aging significantly affects memory and the more critical period for memory process in both young and old animals, particularly at the end of the light period of the circadian cycle. |
| first_indexed | 2024-12-13T02:50:53Z |
| format | Article |
| id | doaj.art-03234fc7a23e4771beb01214d8ff7a8e |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-12-13T02:50:53Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-03234fc7a23e4771beb01214d8ff7a8e2022-12-22T00:02:04ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532018-12-011210.3389/fnbeh.2018.00304437596Time-of-Day and Age Impact on Memory in Elevated Plus-Maze Test in RatsNicanor Morales-Delgado0Nicanor Morales-Delgado1Natalija Popović2Natalija Popović3Ernesto De la Cruz-Sánchez4María Caballero Bleda5María Caballero Bleda6Miroljub Popović7Miroljub Popović8Department of Human Anatomy and Psychobiology, Faculty of Medicine, University of Murcia, Murcia, SpainInstitute of Biomedical Research of Murcia (IMIB), Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, SpainDepartment of Human Anatomy and Psychobiology, Faculty of Medicine, University of Murcia, Murcia, SpainInstitute of Biomedical Research of Murcia (IMIB), Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, SpainDepartment of Physical Activity and Sport, Faculty of Sport Science, University of Murcia, Murcia, SpainDepartment of Human Anatomy and Psychobiology, Faculty of Medicine, University of Murcia, Murcia, SpainInstitute of Biomedical Research of Murcia (IMIB), Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, SpainDepartment of Human Anatomy and Psychobiology, Faculty of Medicine, University of Murcia, Murcia, SpainInstitute of Biomedical Research of Murcia (IMIB), Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, SpainThe purpose of the present study was to establish the effect of daytime and aging on memory in rats in the Elevated Plus-Maze (EPM) test. Young (2-months) and aged (18-months) male Wistar rats were exposed to the EPM test, at the beginning, mid-time or at the end of the light period. On the acquisition trial, the animals were placed individually at the end of one of the open arms of the EPM and the latency to enter in the enclosed arms was registered (cut-off time 60 s). The test was repeated 24 h later on. A longer latency period to reach the enclosed arm indicated poor retention compared to significantly shorter latencies. There were no significant differences between groups on the acquisition trial. In all tested periods, the latency time on the 24 h retention trial was significantly shorter in the young rats compared to the old ones. Furthermore, in the early and mid-time period of the light period, the young rats showed significantly decreased transfer latency (TL) time on the 24 h retention trial in comparison with the acquisition trial. In the aged rats, the TL time on the 24 h retention trial was significantly longer at the end of the light period, in comparison to the two other testing periods. In conclusion, aging significantly affects memory and the more critical period for memory process in both young and old animals, particularly at the end of the light period of the circadian cycle.https://www.frontiersin.org/article/10.3389/fnbeh.2018.00304/fullagingcircadian rhythmelevated plus-mazememoryrat |
| spellingShingle | Nicanor Morales-Delgado Nicanor Morales-Delgado Natalija Popović Natalija Popović Ernesto De la Cruz-Sánchez María Caballero Bleda María Caballero Bleda Miroljub Popović Miroljub Popović Time-of-Day and Age Impact on Memory in Elevated Plus-Maze Test in Rats Frontiers in Behavioral Neuroscience aging circadian rhythm elevated plus-maze memory rat |
| title | Time-of-Day and Age Impact on Memory in Elevated Plus-Maze Test in Rats |
| title_full | Time-of-Day and Age Impact on Memory in Elevated Plus-Maze Test in Rats |
| title_fullStr | Time-of-Day and Age Impact on Memory in Elevated Plus-Maze Test in Rats |
| title_full_unstemmed | Time-of-Day and Age Impact on Memory in Elevated Plus-Maze Test in Rats |
| title_short | Time-of-Day and Age Impact on Memory in Elevated Plus-Maze Test in Rats |
| title_sort | time of day and age impact on memory in elevated plus maze test in rats |
| topic | aging circadian rhythm elevated plus-maze memory rat |
| url | https://www.frontiersin.org/article/10.3389/fnbeh.2018.00304/full |
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