The effects of angiotensin II receptor antagonist (candesartan) on rat renal vascular resistance

The present study aimed to investigate the action of angiotensin II (AII) on renal perfusion pressure and renal vascular resistance using noncompetitive AT1-receptor antagonist (candesartan or CV 11974). Experiments were performed in isolated kidney of adult male Wistar rats. Kreb's Henseleit s...

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Main Authors: Supatraviwat, J, Hiranphun, P., Hiranyachattada, S.
Format: Article
Language:English
Published: Prince of Songkla University 2004-05-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://www.sjst.psu.ac.th/journal/26-4pdf/06-rat.pdf
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author Supatraviwat, J
Hiranphun, P.
Hiranyachattada, S.
author_facet Supatraviwat, J
Hiranphun, P.
Hiranyachattada, S.
author_sort Supatraviwat, J
collection DOAJ
description The present study aimed to investigate the action of angiotensin II (AII) on renal perfusion pressure and renal vascular resistance using noncompetitive AT1-receptor antagonist (candesartan or CV 11974). Experiments were performed in isolated kidney of adult male Wistar rats. Kreb's Henseleit solution was perfused into the renal artery at the rate of 3.5 ml/min. This flow rate was designed in order to maintain renal perfusion pressure between 80-120 mm Hg. Dose-response relationship between perfusion flow rate and AII concentration were studied. Renal perfusion pressure in response to 1, 10 and 100 nM AII were increased from basal perfusion pressure of 94±8 mm Hg to 127±6, 157±12 and 190±16 mm Hg, respectively. Administration of perfusate containing 11.4 μM candesartan for 30 min had no effect on the basal perfusion pressure. However, this significantly reduced renal perfusion pressure in the presence of AII (1, 10 and 100 nM) by 39%, 47% and 61%, (n=7, P<0.05) respectively. At the basal perfusion pressure, calculated renal vascular resistance was 27±2 mm Hg · min · ml-1. However, the vascular resistance were found to be 41±1, 45±2 and 47±2 mm Hg · min · ml-1 when 1, 10 and 100 nM AII were added. Moreover, this dose of candesartan also showed a significant decrease in renal vascular resistance at the corresponding doses of AII by 38%, 48% and 43%, (n=7, P<0.05) respectively. The higher dose of candesartan (22.7 μM) completely inhibited the action of 1, 10 and 100 nM AII on renal vasoconstriction. These results may indicate that the action of AII on renal vascular resistance is via AT1-receptor, at least in rat isolated perfusion kidney.
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spelling doaj.art-032384e03ba4441989c6490e87f34ebf2022-12-21T22:54:22ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952004-05-01264485496The effects of angiotensin II receptor antagonist (candesartan) on rat renal vascular resistanceSupatraviwat, JHiranphun, P.Hiranyachattada, S.The present study aimed to investigate the action of angiotensin II (AII) on renal perfusion pressure and renal vascular resistance using noncompetitive AT1-receptor antagonist (candesartan or CV 11974). Experiments were performed in isolated kidney of adult male Wistar rats. Kreb's Henseleit solution was perfused into the renal artery at the rate of 3.5 ml/min. This flow rate was designed in order to maintain renal perfusion pressure between 80-120 mm Hg. Dose-response relationship between perfusion flow rate and AII concentration were studied. Renal perfusion pressure in response to 1, 10 and 100 nM AII were increased from basal perfusion pressure of 94±8 mm Hg to 127±6, 157±12 and 190±16 mm Hg, respectively. Administration of perfusate containing 11.4 μM candesartan for 30 min had no effect on the basal perfusion pressure. However, this significantly reduced renal perfusion pressure in the presence of AII (1, 10 and 100 nM) by 39%, 47% and 61%, (n=7, P<0.05) respectively. At the basal perfusion pressure, calculated renal vascular resistance was 27±2 mm Hg · min · ml-1. However, the vascular resistance were found to be 41±1, 45±2 and 47±2 mm Hg · min · ml-1 when 1, 10 and 100 nM AII were added. Moreover, this dose of candesartan also showed a significant decrease in renal vascular resistance at the corresponding doses of AII by 38%, 48% and 43%, (n=7, P<0.05) respectively. The higher dose of candesartan (22.7 μM) completely inhibited the action of 1, 10 and 100 nM AII on renal vasoconstriction. These results may indicate that the action of AII on renal vascular resistance is via AT1-receptor, at least in rat isolated perfusion kidney.http://www.sjst.psu.ac.th/journal/26-4pdf/06-rat.pdfAT1- receptor antagonistcandesartan (CV 11974)renal perfusion pressurerenal vascular resistance
spellingShingle Supatraviwat, J
Hiranphun, P.
Hiranyachattada, S.
The effects of angiotensin II receptor antagonist (candesartan) on rat renal vascular resistance
Songklanakarin Journal of Science and Technology (SJST)
AT1- receptor antagonist
candesartan (CV 11974)
renal perfusion pressure
renal vascular resistance
title The effects of angiotensin II receptor antagonist (candesartan) on rat renal vascular resistance
title_full The effects of angiotensin II receptor antagonist (candesartan) on rat renal vascular resistance
title_fullStr The effects of angiotensin II receptor antagonist (candesartan) on rat renal vascular resistance
title_full_unstemmed The effects of angiotensin II receptor antagonist (candesartan) on rat renal vascular resistance
title_short The effects of angiotensin II receptor antagonist (candesartan) on rat renal vascular resistance
title_sort effects of angiotensin ii receptor antagonist candesartan on rat renal vascular resistance
topic AT1- receptor antagonist
candesartan (CV 11974)
renal perfusion pressure
renal vascular resistance
url http://www.sjst.psu.ac.th/journal/26-4pdf/06-rat.pdf
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