Changes in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian Tissue
Salmonellosis caused by Salmonella enteritidis (S. enteritidis) is a worldwide problem in recent years affecting the poultry production as well as public health. The ovarian immunity may play significant role in preventing such contamination because transmission of the bacteria in the ovary has bee...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2004-10-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/41/4/41_4_281/_pdf/-char/en |
| _version_ | 1797816910496137216 |
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| author | Animesh Barua Yukinori Yoshimura |
| author_facet | Animesh Barua Yukinori Yoshimura |
| author_sort | Animesh Barua |
| collection | DOAJ |
| description | Salmonellosis caused by Salmonella enteritidis (S. enteritidis) is a worldwide problem in recent years affecting the poultry production as well as public health. The ovarian immunity may play significant role in preventing such contamination because transmission of the bacteria in the ovary has been suggested as one of the routes of contamination of eggs. The aim of this study was to determine whether the expression of major histocompatibility complex class II (MHC-II) mRNA changes in response to S. enteritidis invasion of cultured ovarian tissues of laying hens. Ovarian stroma and the theca layer of small white follicles (SWF), the largest and third largest follicles (F1 and F3) were incubated with PBS (control), 1×103 S. enteritidis (SE-low group) or 1×106 S. enteritidis (SE-high group) for 0h, 2h and 4h in vitro. The expressions of MHC-II mRNA in these tissues were determined by reverse transcription-polymerase chain reaction (RT-PCR). MHC-II mRNA of a product size of 210bp was observed in ovarian stroma and follicular tissues. The expression of MHC-II mRNA was significantly increased following 4h of incubation with S. enteritidis in both SE-low and -high groups but not by 2h of incubation. Significant differences were not observed in the mRNA expression between SE-low and -high groups within the corresponding time regime. The expression of MHC-II mRNA was not significantly different among the SWF, F3 and F1 follicles incubated for same period and received similar doses of S. enteritidis bacteria. These results indicate that MHC-II mRNAs were expressed in the ovarian tissues and their expressions were increased in response to S. enteritidis invasion in these tissues and hen ovary is an inductive sites for immunoresponse against the invading S. enteritidis bacteria. |
| first_indexed | 2024-03-13T08:44:46Z |
| format | Article |
| id | doaj.art-0327de6c283046a085459aa6b7441155 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:44:46Z |
| publishDate | 2004-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-0327de6c283046a085459aa6b74411552023-05-30T06:07:37ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862004-10-0141428128810.2141/jpsa.41.281jpsaChanges in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian TissueAnimesh Barua0Yukinori Yoshimura1Graduate School of Biosphere Science, Hiroshima UniversityGraduate School of Biosphere Science, Hiroshima UniversitySalmonellosis caused by Salmonella enteritidis (S. enteritidis) is a worldwide problem in recent years affecting the poultry production as well as public health. The ovarian immunity may play significant role in preventing such contamination because transmission of the bacteria in the ovary has been suggested as one of the routes of contamination of eggs. The aim of this study was to determine whether the expression of major histocompatibility complex class II (MHC-II) mRNA changes in response to S. enteritidis invasion of cultured ovarian tissues of laying hens. Ovarian stroma and the theca layer of small white follicles (SWF), the largest and third largest follicles (F1 and F3) were incubated with PBS (control), 1×103 S. enteritidis (SE-low group) or 1×106 S. enteritidis (SE-high group) for 0h, 2h and 4h in vitro. The expressions of MHC-II mRNA in these tissues were determined by reverse transcription-polymerase chain reaction (RT-PCR). MHC-II mRNA of a product size of 210bp was observed in ovarian stroma and follicular tissues. The expression of MHC-II mRNA was significantly increased following 4h of incubation with S. enteritidis in both SE-low and -high groups but not by 2h of incubation. Significant differences were not observed in the mRNA expression between SE-low and -high groups within the corresponding time regime. The expression of MHC-II mRNA was not significantly different among the SWF, F3 and F1 follicles incubated for same period and received similar doses of S. enteritidis bacteria. These results indicate that MHC-II mRNAs were expressed in the ovarian tissues and their expressions were increased in response to S. enteritidis invasion in these tissues and hen ovary is an inductive sites for immunoresponse against the invading S. enteritidis bacteria.https://www.jstage.jst.go.jp/article/jpsa/41/4/41_4_281/_pdf/-char/ensalmonella enteritidismhc-ii mrnaovaryfolliclehen |
| spellingShingle | Animesh Barua Yukinori Yoshimura Changes in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian Tissue The Journal of Poultry Science salmonella enteritidis mhc-ii mrna ovary follicle hen |
| title | Changes in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian Tissue |
| title_full | Changes in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian Tissue |
| title_fullStr | Changes in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian Tissue |
| title_full_unstemmed | Changes in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian Tissue |
| title_short | Changes in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian Tissue |
| title_sort | changes in the expression of major histocompatibility complex class ii mrna in response to inoculation with salmonella enteritidis in cultured hen ovarian tissue |
| topic | salmonella enteritidis mhc-ii mrna ovary follicle hen |
| url | https://www.jstage.jst.go.jp/article/jpsa/41/4/41_4_281/_pdf/-char/en |
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