Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, Nigeria
Although Escherichia coli (E. coli) is a harmless gut microbe in man, some strains of this bacterium are pathogenic due to the acquisition of virulence factors. The aim of this study is to investigate E. coli O157:H7 strains isolated from Ogun River, Abeokuta metropolis, for virulence factors and an...
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| Format: | Article |
| Language: | English |
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Environmental Research Institute, Chulalongkorn University
2019-02-01
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| Series: | Applied Environmental Research |
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| Online Access: | https://ph01.tci-thaijo.org/jer/index.php/aer/article/view/153466 |
| _version_ | 1797294854484525056 |
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| author | Lateefat M. Ewuoso Saka A. Balogun Sarafadeen O. Kareem Temilade F. Akinhanmi |
| author_facet | Lateefat M. Ewuoso Saka A. Balogun Sarafadeen O. Kareem Temilade F. Akinhanmi |
| author_sort | Lateefat M. Ewuoso |
| collection | DOAJ |
| description | Although Escherichia coli (E. coli) is a harmless gut microbe in man, some strains of this bacterium are pathogenic due to the acquisition of virulence factors. The aim of this study is to investigate E. coli O157:H7 strains isolated from Ogun River, Abeokuta metropolis, for virulence factors and antibiotic resistance. Water samples were collected bimonthly from six different locations over a period of six months. The samples were cultured on Sorbitol MacConkey Agar and E. coli O157:H7 isolates were confirmed through serological characterization using the latex agglutination test. The presence of virulence genes (stx1, stx2, eae, and hylA) in the isolates was analyzed through polymerase chain reaction (PCR). Further, antibiotic susceptibility of the isolates was tested using the disc diffusion method. The PCR analysis revealed that the five E. coli O157:H7 strains isolated possessed Shiga toxin 1 (stx1), Shiga toxin 2 (stx2), and Haemolysin (hlyA) genes. Additionally, the isolates were resistant to Augmentin, Ceftriaxone, Nitrofurantoin, Gentamycin, Amoxicillin, and Pefloxacin. This study shows that E. coli O157:H7 strains are present in Ogun River and that these strains possess multiple virulence factors and are resistant to multiple drugs.
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| first_indexed | 2024-03-07T21:37:10Z |
| format | Article |
| id | doaj.art-032930aa36334e039d78228669e8caa0 |
| institution | Directory Open Access Journal |
| issn | 2287-075X |
| language | English |
| last_indexed | 2024-03-07T21:37:10Z |
| publishDate | 2019-02-01 |
| publisher | Environmental Research Institute, Chulalongkorn University |
| record_format | Article |
| series | Applied Environmental Research |
| spelling | doaj.art-032930aa36334e039d78228669e8caa02024-02-26T10:50:11ZengEnvironmental Research Institute, Chulalongkorn UniversityApplied Environmental Research2287-075X2019-02-0141110.35762/AER.2019.41.1.3Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, NigeriaLateefat M. Ewuoso0Saka A. Balogun1Sarafadeen O. Kareem2Temilade F. Akinhanmi3College of Biosciences, Federal University of Agriculture, Abeokuta, NigeriaCollege of Biosciences, Federal University of Agriculture, Abeokuta, NigeriaCollege of Biosciences, Federal University of Agriculture, Abeokuta, NigeriaCollege of Physical Sciences, Federal University of Agriculture, Abeokuta, NigeriaAlthough Escherichia coli (E. coli) is a harmless gut microbe in man, some strains of this bacterium are pathogenic due to the acquisition of virulence factors. The aim of this study is to investigate E. coli O157:H7 strains isolated from Ogun River, Abeokuta metropolis, for virulence factors and antibiotic resistance. Water samples were collected bimonthly from six different locations over a period of six months. The samples were cultured on Sorbitol MacConkey Agar and E. coli O157:H7 isolates were confirmed through serological characterization using the latex agglutination test. The presence of virulence genes (stx1, stx2, eae, and hylA) in the isolates was analyzed through polymerase chain reaction (PCR). Further, antibiotic susceptibility of the isolates was tested using the disc diffusion method. The PCR analysis revealed that the five E. coli O157:H7 strains isolated possessed Shiga toxin 1 (stx1), Shiga toxin 2 (stx2), and Haemolysin (hlyA) genes. Additionally, the isolates were resistant to Augmentin, Ceftriaxone, Nitrofurantoin, Gentamycin, Amoxicillin, and Pefloxacin. This study shows that E. coli O157:H7 strains are present in Ogun River and that these strains possess multiple virulence factors and are resistant to multiple drugs. .https://ph01.tci-thaijo.org/jer/index.php/aer/article/view/153466Escherichia coliVirulence factorShiga toxinAntibiotic resistanceOgun River |
| spellingShingle | Lateefat M. Ewuoso Saka A. Balogun Sarafadeen O. Kareem Temilade F. Akinhanmi Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, Nigeria Applied Environmental Research Escherichia coli Virulence factor Shiga toxin Antibiotic resistance Ogun River |
| title | Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, Nigeria |
| title_full | Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, Nigeria |
| title_fullStr | Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, Nigeria |
| title_full_unstemmed | Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, Nigeria |
| title_short | Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, Nigeria |
| title_sort | virulence factors and antibiotic resistance of escherichia coli o157 h7 isolated from ogun river abeokuta metropolis nigeria |
| topic | Escherichia coli Virulence factor Shiga toxin Antibiotic resistance Ogun River |
| url | https://ph01.tci-thaijo.org/jer/index.php/aer/article/view/153466 |
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