A genome-wide association study of folates in sweet corn kernels
Folate is commonly synthesized in natural plants and is an essential water-soluble vitamin of great importance inhuman health. Although the key genes involved in folate biosynthesis and transformation pathways have been identified in plants, the genetic architecture of folate in sweet corn kernels r...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1004455/full |
| _version_ | 1811206890778001408 |
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| author | Yingni Xiao Yongtao Yu Lihua Xie Kun Li Xinbo Guo Guangyu Li Jianhua Liu Gaoke Li Jianguang Hu |
| author_facet | Yingni Xiao Yongtao Yu Lihua Xie Kun Li Xinbo Guo Guangyu Li Jianhua Liu Gaoke Li Jianguang Hu |
| author_sort | Yingni Xiao |
| collection | DOAJ |
| description | Folate is commonly synthesized in natural plants and is an essential water-soluble vitamin of great importance inhuman health. Although the key genes involved in folate biosynthesis and transformation pathways have been identified in plants, the genetic architecture of folate in sweet corn kernels remain largely unclear. In this study, an association panel of 295 inbred lines of sweet corn was constructed. Six folate derivatives were quantified in sweet corn kernels at 20 days after pollination and a total of 95 loci were identified for eight folate traits using a genome-wide association study. A peak GWAS signal revealed that natural variation in ZmFCL, encoding a 5-formyltetrahydrofolate cyclo-ligase, accounted for 30.12% of phenotypic variation in 5-FTHF content. Further analysis revealed that two adjacent SNPs on the second exon resulting in an AA-to-GG in the gene and an Asn-to-Gly change in the protein could be the causative variant influencing 5-FTHF content. Meanwhile, 5-FTHF content was negatively correlated with ZmFCL expression levels in the population. These results extend our knowledge regarding the genetic basis of folate and provide molecular markers for the optimization of folate levels in sweet corn kernels. |
| first_indexed | 2024-04-12T03:54:37Z |
| format | Article |
| id | doaj.art-03297f2a37024f12a6a3d22a32c04eee |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T03:54:37Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-03297f2a37024f12a6a3d22a32c04eee2022-12-22T03:48:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10044551004455A genome-wide association study of folates in sweet corn kernelsYingni Xiao0Yongtao Yu1Lihua Xie2Kun Li3Xinbo Guo4Guangyu Li5Jianhua Liu6Gaoke Li7Jianguang Hu8Crops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, ChinaCrops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, ChinaCrops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, ChinaCrops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou, ChinaCrops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, ChinaCrops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, ChinaCrops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, ChinaCrops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou, ChinaFolate is commonly synthesized in natural plants and is an essential water-soluble vitamin of great importance inhuman health. Although the key genes involved in folate biosynthesis and transformation pathways have been identified in plants, the genetic architecture of folate in sweet corn kernels remain largely unclear. In this study, an association panel of 295 inbred lines of sweet corn was constructed. Six folate derivatives were quantified in sweet corn kernels at 20 days after pollination and a total of 95 loci were identified for eight folate traits using a genome-wide association study. A peak GWAS signal revealed that natural variation in ZmFCL, encoding a 5-formyltetrahydrofolate cyclo-ligase, accounted for 30.12% of phenotypic variation in 5-FTHF content. Further analysis revealed that two adjacent SNPs on the second exon resulting in an AA-to-GG in the gene and an Asn-to-Gly change in the protein could be the causative variant influencing 5-FTHF content. Meanwhile, 5-FTHF content was negatively correlated with ZmFCL expression levels in the population. These results extend our knowledge regarding the genetic basis of folate and provide molecular markers for the optimization of folate levels in sweet corn kernels.https://www.frontiersin.org/articles/10.3389/fpls.2022.1004455/fullsweet cornfolatesgenome-wide association studygenetic basisformyltetrahydrofolate cyclo-ligase |
| spellingShingle | Yingni Xiao Yongtao Yu Lihua Xie Kun Li Xinbo Guo Guangyu Li Jianhua Liu Gaoke Li Jianguang Hu A genome-wide association study of folates in sweet corn kernels Frontiers in Plant Science sweet corn folates genome-wide association study genetic basis formyltetrahydrofolate cyclo-ligase |
| title | A genome-wide association study of folates in sweet corn kernels |
| title_full | A genome-wide association study of folates in sweet corn kernels |
| title_fullStr | A genome-wide association study of folates in sweet corn kernels |
| title_full_unstemmed | A genome-wide association study of folates in sweet corn kernels |
| title_short | A genome-wide association study of folates in sweet corn kernels |
| title_sort | genome wide association study of folates in sweet corn kernels |
| topic | sweet corn folates genome-wide association study genetic basis formyltetrahydrofolate cyclo-ligase |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1004455/full |
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