Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude
BackgroundIntestinal microbiota plays an important role in maintaining the microecological balance of the gastrointestinal tract in various animals. Disturbances in the intestinal microbiota may lead to the proliferation of potentially pathogenic bacteria that become the dominant species, leading to...
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| Format: | Article |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1007737/full |
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| author | Zhiqiang Wan Xufei Zhang Xianhao Jia Yuhua Qin Ning Sun Jinge Xin Yan Zeng Bo Jing Jing Fang Kangcheng Pan Dong Zeng Yang Bai Hesong Wang Hailin Ma Xueqin Ni |
| author_facet | Zhiqiang Wan Xufei Zhang Xianhao Jia Yuhua Qin Ning Sun Jinge Xin Yan Zeng Bo Jing Jing Fang Kangcheng Pan Dong Zeng Yang Bai Hesong Wang Hailin Ma Xueqin Ni |
| author_sort | Zhiqiang Wan |
| collection | DOAJ |
| description | BackgroundIntestinal microbiota plays an important role in maintaining the microecological balance of the gastrointestinal tract in various animals. Disturbances in the intestinal microbiota may lead to the proliferation of potentially pathogenic bacteria that become the dominant species, leading to intestinal immune disorders, intestinal inflammation, and other intestinal diseases. Numerous studies have been confirmed that high-altitude exposure affects the normal function of the intestine and the composition of the intestinal microbiota. However, it is still necessary to reveal the changes in intestinal microbiota in high-altitude exposure environments, and clarify the relationship between the proliferation of potentially pathogenic bacteria and intestinal injury in this environment. In addition, explored probiotics that may have preventive effects against intestinal diseases.Methods and resultsC57BL/6 mice were randomly divided into three groups, a high-altitude group (HA), control group (C), and high-altitude probiotic group (HAP). The HA and HAP groups were subjected to hypoxia modeling for 14 days in a low-pressure oxygen chamber with daily gavage of 0.2 mL of normal saline (HA) and Lactobacillus johnsonii YH1136 bacterial fluid (HAP), while the control group was fed normally. L. johnsonii YH1136 was isolated from feces of a healthy Tibetan girl in Baingoin county, the Nagqu region of the Tibet Autonomous Region, at an altitude of 5000 meters. Our observations revealed that gavage of YH1136 was effective in improving the damage to the intestinal barrier caused by high-altitude exposure to hypoxic environments and helped to reduce the likelihood of pathogenic bacteria infection through the intestinal barrier. It also positively regulates the intestinal microbiota to the extent of Lactobacillus being the dominant microbiome and reducing the number of pathogenic bacteria. By analyzing the expression profile of ileal microRNAs and correlation analysis with intestinal microbiota, we found that Staphylococcus and Corynebacterium1 cooperated with miR-196a-1-3p and miR-3060-3p, respectively, to play a regulatory role in the process of high-altitude hypoxia-induced intestinal injury.ConclusionThese findings revealed the beneficial effect of L. johnsonii YH1136 in preventing potential endogenous pathogenic bacteria-induced intestinal dysfunction in high-altitude environments. The mechanism may be related to the regulation of intestinal injury from the perspective of the gut microbiota as well as miRNAs. |
| first_indexed | 2024-04-12T00:38:03Z |
| format | Article |
| id | doaj.art-032ed27f6de84fcc8e445165d1a2e81a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T00:38:03Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-032ed27f6de84fcc8e445165d1a2e81a2022-12-22T03:55:05ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-10-011310.3389/fimmu.2022.10077371007737Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitudeZhiqiang Wan0Xufei Zhang1Xianhao Jia2Yuhua Qin3Ning Sun4Jinge Xin5Yan Zeng6Bo Jing7Jing Fang8Kangcheng Pan9Dong Zeng10Yang Bai11Hesong Wang12Hailin Ma13Xueqin Ni14Animal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu, ChinaPlateau Brain Science Research Center, Tibet University, Lhasa, ChinaPlateau Brain Science Research Center, Tibet University, Lhasa, ChinaCollege of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaAnimal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaAnimal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu, ChinaAnimal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu, ChinaAnimal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu, ChinaAnimal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu, ChinaAnimal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangzhou Beneco Biotechnology Co. Ltd., Guangzhou, ChinaPlateau Brain Science Research Center, Tibet University, Lhasa, ChinaAnimal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu, ChinaBackgroundIntestinal microbiota plays an important role in maintaining the microecological balance of the gastrointestinal tract in various animals. Disturbances in the intestinal microbiota may lead to the proliferation of potentially pathogenic bacteria that become the dominant species, leading to intestinal immune disorders, intestinal inflammation, and other intestinal diseases. Numerous studies have been confirmed that high-altitude exposure affects the normal function of the intestine and the composition of the intestinal microbiota. However, it is still necessary to reveal the changes in intestinal microbiota in high-altitude exposure environments, and clarify the relationship between the proliferation of potentially pathogenic bacteria and intestinal injury in this environment. In addition, explored probiotics that may have preventive effects against intestinal diseases.Methods and resultsC57BL/6 mice were randomly divided into three groups, a high-altitude group (HA), control group (C), and high-altitude probiotic group (HAP). The HA and HAP groups were subjected to hypoxia modeling for 14 days in a low-pressure oxygen chamber with daily gavage of 0.2 mL of normal saline (HA) and Lactobacillus johnsonii YH1136 bacterial fluid (HAP), while the control group was fed normally. L. johnsonii YH1136 was isolated from feces of a healthy Tibetan girl in Baingoin county, the Nagqu region of the Tibet Autonomous Region, at an altitude of 5000 meters. Our observations revealed that gavage of YH1136 was effective in improving the damage to the intestinal barrier caused by high-altitude exposure to hypoxic environments and helped to reduce the likelihood of pathogenic bacteria infection through the intestinal barrier. It also positively regulates the intestinal microbiota to the extent of Lactobacillus being the dominant microbiome and reducing the number of pathogenic bacteria. By analyzing the expression profile of ileal microRNAs and correlation analysis with intestinal microbiota, we found that Staphylococcus and Corynebacterium1 cooperated with miR-196a-1-3p and miR-3060-3p, respectively, to play a regulatory role in the process of high-altitude hypoxia-induced intestinal injury.ConclusionThese findings revealed the beneficial effect of L. johnsonii YH1136 in preventing potential endogenous pathogenic bacteria-induced intestinal dysfunction in high-altitude environments. The mechanism may be related to the regulation of intestinal injury from the perspective of the gut microbiota as well as miRNAs.https://www.frontiersin.org/articles/10.3389/fimmu.2022.1007737/fullhigh-altitude exposureprobioticLactobacillus johnsoniiintestinal microbiotamiRNA |
| spellingShingle | Zhiqiang Wan Xufei Zhang Xianhao Jia Yuhua Qin Ning Sun Jinge Xin Yan Zeng Bo Jing Jing Fang Kangcheng Pan Dong Zeng Yang Bai Hesong Wang Hailin Ma Xueqin Ni Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude Frontiers in Immunology high-altitude exposure probiotic Lactobacillus johnsonii intestinal microbiota miRNA |
| title | Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude |
| title_full | Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude |
| title_fullStr | Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude |
| title_full_unstemmed | Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude |
| title_short | Lactobacillus johnsonii YH1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude |
| title_sort | lactobacillus johnsonii yh1136 plays a protective role against endogenous pathogenic bacteria induced intestinal dysfunction by reconstructing gut microbiota in mice exposed at high altitude |
| topic | high-altitude exposure probiotic Lactobacillus johnsonii intestinal microbiota miRNA |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1007737/full |
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