Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in Parents
Background: Co-occurrence of complex copy number variants (CNVs) is associated with more severe clinical expressivity of known syndromes. Few studies discuss diagnosis and genetic counseling for fetuses identified with multiple CNVs. This cohort study aims to summarize findings of complex copy numbe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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IMR Press
2023-10-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/50/10/10.31083/j.ceog5010215 |
| _version_ | 1797643641183797248 |
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| author | Wei Wang Jing Wang Ye Shi Bin Zhang |
| author_facet | Wei Wang Jing Wang Ye Shi Bin Zhang |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Background: Co-occurrence of complex copy number variants (CNVs) is associated with more severe clinical expressivity of known syndromes. Few studies discuss diagnosis and genetic counseling for fetuses identified with multiple CNVs. This cohort study aims to summarize findings of complex copy number variants identified via prenatal diagnosis along with the results of parental studies. Methods: 2746 pregnant women were included and diagnosed by chromosomal microarray analysis (CMA) according to different clinical indications. A total of 12 fetuses were diagnosed with complex CNVs (a fetus identified with two or more CNVs simultaneously). Parental analysis was performed by CMA, G-band karyotype analysis, and whole-genome low-coverage mate-pair sequencing (WGL-MPS) based on the size of the fetal imbalances and method resolution. Results: Fetuses carrying complex CNVs were identified as being 0.4% (12/2746) in our cohort. The parental validation study was performed in 8 of 12 complex CNVs cases with the permission of the patients. The primary results suggested that 62.5% (5 out of 8) of fetuses with complex CNVs were from parental inheritance. In these cases, 4 out of 5 were derived from maternal or paternal balanced translocation carriers. Recurrent spontaneous abortion was found in balanced translocation carrier family. Conclusion: In this study, in 4/8 of the fetuses detected with complex CNVs was inherited from a parental balanced translocation. Given the risk of parental balanced rearrangements when fetal complex CNVs are identified, genetic counseling for future pregnancies may be useful for these families. |
| first_indexed | 2024-03-11T14:17:51Z |
| format | Article |
| id | doaj.art-0332a25cb56247d6b73b60f70dd014ca |
| institution | Directory Open Access Journal |
| issn | 0390-6663 |
| language | English |
| last_indexed | 2024-03-11T14:17:51Z |
| publishDate | 2023-10-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj.art-0332a25cb56247d6b73b60f70dd014ca2023-11-01T03:27:21ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632023-10-01501021510.31083/j.ceog5010215S0390-6663(23)02171-1Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in ParentsWei Wang0Jing Wang1Ye Shi2Bin Zhang3Department of Medical Genetics, Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Centre, Nanjing Medical University, 213000 Changzhou, Jiangsu, ChinaDepartment of Medical Genetics, Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Centre, Nanjing Medical University, 213000 Changzhou, Jiangsu, ChinaDepartment of Medical Genetics, Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Centre, Nanjing Medical University, 213000 Changzhou, Jiangsu, ChinaDepartment of Medical Genetics, Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Centre, Nanjing Medical University, 213000 Changzhou, Jiangsu, ChinaBackground: Co-occurrence of complex copy number variants (CNVs) is associated with more severe clinical expressivity of known syndromes. Few studies discuss diagnosis and genetic counseling for fetuses identified with multiple CNVs. This cohort study aims to summarize findings of complex copy number variants identified via prenatal diagnosis along with the results of parental studies. Methods: 2746 pregnant women were included and diagnosed by chromosomal microarray analysis (CMA) according to different clinical indications. A total of 12 fetuses were diagnosed with complex CNVs (a fetus identified with two or more CNVs simultaneously). Parental analysis was performed by CMA, G-band karyotype analysis, and whole-genome low-coverage mate-pair sequencing (WGL-MPS) based on the size of the fetal imbalances and method resolution. Results: Fetuses carrying complex CNVs were identified as being 0.4% (12/2746) in our cohort. The parental validation study was performed in 8 of 12 complex CNVs cases with the permission of the patients. The primary results suggested that 62.5% (5 out of 8) of fetuses with complex CNVs were from parental inheritance. In these cases, 4 out of 5 were derived from maternal or paternal balanced translocation carriers. Recurrent spontaneous abortion was found in balanced translocation carrier family. Conclusion: In this study, in 4/8 of the fetuses detected with complex CNVs was inherited from a parental balanced translocation. Given the risk of parental balanced rearrangements when fetal complex CNVs are identified, genetic counseling for future pregnancies may be useful for these families.https://www.imrpress.com/journal/CEOG/50/10/10.31083/j.ceog5010215complex cnvsprenatal diagnosisgenetic counselingbalanced translocationparental analysis |
| spellingShingle | Wei Wang Jing Wang Ye Shi Bin Zhang Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in Parents Clinical and Experimental Obstetrics & Gynecology complex cnvs prenatal diagnosis genetic counseling balanced translocation parental analysis |
| title | Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in Parents |
| title_full | Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in Parents |
| title_fullStr | Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in Parents |
| title_full_unstemmed | Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in Parents |
| title_short | Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in Parents |
| title_sort | prenatal diagnosis of complex copy number variants in the fetus and associated cytogenetic findings in parents |
| topic | complex cnvs prenatal diagnosis genetic counseling balanced translocation parental analysis |
| url | https://www.imrpress.com/journal/CEOG/50/10/10.31083/j.ceog5010215 |
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