In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing
Abstract The recent human Monkeypox outbreak underlined the importance of studying basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Se...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-023-02149-4 |
| _version_ | 1797827683547086848 |
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| author | Balázs Kakuk Ákos Dörmő Zsolt Csabai Gábor Kemenesi Jiří Holoubek Daniel Růžek István Prazsák Virág Éva Dani Béla Dénes Gábor Torma Ferenc Jakab Gábor E. Tóth Fanni V. Földes Brigitta Zana Zsófia Lanszki Ákos Harangozó Ádám Fülöp Gábor Gulyás Máté Mizik András Attila Kiss Dóra Tombácz Zsolt Boldogkői |
| author_facet | Balázs Kakuk Ákos Dörmő Zsolt Csabai Gábor Kemenesi Jiří Holoubek Daniel Růžek István Prazsák Virág Éva Dani Béla Dénes Gábor Torma Ferenc Jakab Gábor E. Tóth Fanni V. Földes Brigitta Zana Zsófia Lanszki Ákos Harangozó Ádám Fülöp Gábor Gulyás Máté Mizik András Attila Kiss Dóra Tombácz Zsolt Boldogkői |
| author_sort | Balázs Kakuk |
| collection | DOAJ |
| description | Abstract The recent human Monkeypox outbreak underlined the importance of studying basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. It will enable the in-depth characterization of the transcriptomic architecture of the monkeypox virus, and may even make possible to annotate novel host transcripts. Moreover, our direct cDNA and native RNA sequencing reads will allow the estimation of gene expression changes of both the virus and the host cells during the infection. Overall, our study will lead to a deeper understanding of the alterations caused by the viral infection on a transcriptome level. |
| first_indexed | 2024-04-09T12:52:17Z |
| format | Article |
| id | doaj.art-0336c57707aa4d108acaceffd5d1637e |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-04-09T12:52:17Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj.art-0336c57707aa4d108acaceffd5d1637e2023-05-14T11:07:57ZengNature PortfolioScientific Data2052-44632023-05-0110111210.1038/s41597-023-02149-4In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore SequencingBalázs Kakuk0Ákos Dörmő1Zsolt Csabai2Gábor Kemenesi3Jiří Holoubek4Daniel Růžek5István Prazsák6Virág Éva Dani7Béla Dénes8Gábor Torma9Ferenc Jakab10Gábor E. Tóth11Fanni V. Földes12Brigitta Zana13Zsófia Lanszki14Ákos Harangozó15Ádám Fülöp16Gábor Gulyás17Máté Mizik18András Attila Kiss19Dóra Tombácz20Zsolt Boldogkői21Department of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedNational Laboratory of Virology, Szentágothai Research Centre, University of PécsVeterinary Research InstituteVeterinary Research InstituteDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine BudapestDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedNational Laboratory of Virology, Szentágothai Research Centre, University of PécsNational Laboratory of Virology, Szentágothai Research Centre, University of PécsNational Laboratory of Virology, Szentágothai Research Centre, University of PécsNational Laboratory of Virology, Szentágothai Research Centre, University of PécsNational Laboratory of Virology, Szentágothai Research Centre, University of PécsDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedDepartment of Medical Biology, Albert Szent-Györgyi Medical School, University of SzegedAbstract The recent human Monkeypox outbreak underlined the importance of studying basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. It will enable the in-depth characterization of the transcriptomic architecture of the monkeypox virus, and may even make possible to annotate novel host transcripts. Moreover, our direct cDNA and native RNA sequencing reads will allow the estimation of gene expression changes of both the virus and the host cells during the infection. Overall, our study will lead to a deeper understanding of the alterations caused by the viral infection on a transcriptome level.https://doi.org/10.1038/s41597-023-02149-4 |
| spellingShingle | Balázs Kakuk Ákos Dörmő Zsolt Csabai Gábor Kemenesi Jiří Holoubek Daniel Růžek István Prazsák Virág Éva Dani Béla Dénes Gábor Torma Ferenc Jakab Gábor E. Tóth Fanni V. Földes Brigitta Zana Zsófia Lanszki Ákos Harangozó Ádám Fülöp Gábor Gulyás Máté Mizik András Attila Kiss Dóra Tombácz Zsolt Boldogkői In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing Scientific Data |
| title | In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing |
| title_full | In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing |
| title_fullStr | In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing |
| title_full_unstemmed | In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing |
| title_short | In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing |
| title_sort | in depth temporal transcriptome profiling of monkeypox and host cells using nanopore sequencing |
| url | https://doi.org/10.1038/s41597-023-02149-4 |
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