Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense)
Proso millet and Little millet belong to group of small millets and family poaceae. Flower opening in these crops are chasmogamous type, in which pollination takes place prior to opening of flowers. Hence, self pollination predominates. So, to create variability, hybridization is prerequisite. Bec...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Society of Plant Breeders
2019-09-01
|
| Series: | Electronic Journal of Plant Breeding |
| Subjects: | |
| Online Access: | http://ejplantbreeding.org/index.php/EJPB/article/view/3041 |
| _version_ | 1818134582939090944 |
|---|---|
| author | C. Nandini Sujata Bhat Srinathareddy Jayramegowda Prabhakar |
| author_facet | C. Nandini Sujata Bhat Srinathareddy Jayramegowda Prabhakar |
| author_sort | C. Nandini |
| collection | DOAJ |
| description | Proso millet and Little millet belong to group of small millets and family poaceae. Flower opening in these crops are
chasmogamous type, in which pollination takes place prior to opening of flowers. Hence, self pollination predominates. So,
to create variability, hybridization is prerequisite. Because of self pollination and non availability of male sterility,
emasculation is necessary for crossing. There are many emasculation and crossing methods like contact method, hot water
treatment, hand emasculation and USSR method which are used in these crops. But the disadvantage in these methods is
damage to stigma this reduces the success rate of obtaining true F1’s. To overcome all these problems of earlier methods, in
our present study, we proposed the modified crossing (SMUASB) method. In this method, cold water of 5-80C is sprayed on
the panicle as a mechanical stimulator for opening of florets in male and female panicles. For emasculation, female panicle is
gently washed in cold water. This will not affect stigma and its receptivity. All florets which are already fertilized and not
opened immature florets are removed before pollination. Hence, success rate of obtaining true F1s is more and less land and
resources are required for evaluation of F1’s. Using SMUASB method, we attempted crosses in Little millet and Proso millet
and the success rate observed was 56% and 60% respectively. |
| first_indexed | 2024-12-11T09:10:55Z |
| format | Article |
| id | doaj.art-033706b58061413fa6dd33f260d07f4b |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-12-11T09:10:55Z |
| publishDate | 2019-09-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-033706b58061413fa6dd33f260d07f4b2022-12-22T01:13:29ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2019-09-011031161117010.5958/0975-928X.2019.00147.9Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense)C. NandiniSujata BhatSrinathareddyJayramegowdaPrabhakarProso millet and Little millet belong to group of small millets and family poaceae. Flower opening in these crops are chasmogamous type, in which pollination takes place prior to opening of flowers. Hence, self pollination predominates. So, to create variability, hybridization is prerequisite. Because of self pollination and non availability of male sterility, emasculation is necessary for crossing. There are many emasculation and crossing methods like contact method, hot water treatment, hand emasculation and USSR method which are used in these crops. But the disadvantage in these methods is damage to stigma this reduces the success rate of obtaining true F1’s. To overcome all these problems of earlier methods, in our present study, we proposed the modified crossing (SMUASB) method. In this method, cold water of 5-80C is sprayed on the panicle as a mechanical stimulator for opening of florets in male and female panicles. For emasculation, female panicle is gently washed in cold water. This will not affect stigma and its receptivity. All florets which are already fertilized and not opened immature florets are removed before pollination. Hence, success rate of obtaining true F1s is more and less land and resources are required for evaluation of F1’s. Using SMUASB method, we attempted crosses in Little millet and Proso millet and the success rate observed was 56% and 60% respectively.http://ejplantbreeding.org/index.php/EJPB/article/view/3041ChasmogamousEmasculationHybridizationLittle milletPollinationProso milletSMUASB method |
| spellingShingle | C. Nandini Sujata Bhat Srinathareddy Jayramegowda Prabhakar Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense) Electronic Journal of Plant Breeding Chasmogamous Emasculation Hybridization Little millet Pollination Proso millet SMUASB method |
| title | Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense) |
| title_full | Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense) |
| title_fullStr | Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense) |
| title_full_unstemmed | Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense) |
| title_short | Modified crossing (SMUASB) method for artificial hybridization in proso millet (Panicum miliaceum L.) and Little millet (Panicum sumatrense) |
| title_sort | modified crossing smuasb method for artificial hybridization in proso millet panicum miliaceum l and little millet panicum sumatrense |
| topic | Chasmogamous Emasculation Hybridization Little millet Pollination Proso millet SMUASB method |
| url | http://ejplantbreeding.org/index.php/EJPB/article/view/3041 |
| work_keys_str_mv | AT cnandini modifiedcrossingsmuasbmethodforartificialhybridizationinprosomilletpanicummiliaceumlandlittlemilletpanicumsumatrense AT sujatabhat modifiedcrossingsmuasbmethodforartificialhybridizationinprosomilletpanicummiliaceumlandlittlemilletpanicumsumatrense AT srinathareddy modifiedcrossingsmuasbmethodforartificialhybridizationinprosomilletpanicummiliaceumlandlittlemilletpanicumsumatrense AT jayramegowda modifiedcrossingsmuasbmethodforartificialhybridizationinprosomilletpanicummiliaceumlandlittlemilletpanicumsumatrense AT prabhakar modifiedcrossingsmuasbmethodforartificialhybridizationinprosomilletpanicummiliaceumlandlittlemilletpanicumsumatrense |