Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation
Unlike fungal and bacterial diseases, no direct method is available to control viral diseases. The use of resistance-inducing compounds can be an alternative strategy for plant viruses. Here we studied the basal response of melon to Melon necrotic spot virus (MNSV) and demonstrated the efficacy of h...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01793/full |
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| author | Emma Fernández-Crespo Jose A. Navarro Marta Serra-Soriano Iván Finiti Pilar García-Agustín Vicente Pallás Carmen González-Bosch |
| author_facet | Emma Fernández-Crespo Jose A. Navarro Marta Serra-Soriano Iván Finiti Pilar García-Agustín Vicente Pallás Carmen González-Bosch |
| author_sort | Emma Fernández-Crespo |
| collection | DOAJ |
| description | Unlike fungal and bacterial diseases, no direct method is available to control viral diseases. The use of resistance-inducing compounds can be an alternative strategy for plant viruses. Here we studied the basal response of melon to Melon necrotic spot virus (MNSV) and demonstrated the efficacy of hexanoic acid (Hx) priming, which prevents the virus from systemically spreading. We analysed callose deposition and the hormonal profile and gene expression at the whole plant level. This allowed us to determine hormonal homeostasis in the melon roots, cotyledons, hypocotyls, stems and leaves involved in basal and hexanoic acid-induced resistance (Hx-IR) to MNSV. Our data indicate important roles of salicylic acid (SA), 12-oxo-phytodienoic acid (OPDA), jasmonic-isoleucine, and ferulic acid in both responses to MNSV. The hormonal and metabolites balance, depending on the time and location associated with basal and Hx-IR, demonstrated the reprogramming of plant metabolism in MNSV-inoculated plants. The treatment with both SA and OPDA prior to virus infection significantly reduced MNSV systemic movement by inducing callose deposition. This demonstrates their relevance in Hx-IR against MNSV and a high correlation with callose deposition. Our data also provide valuable evidence to unravel priming mechanisms by natural compounds. |
| first_indexed | 2024-12-23T13:15:28Z |
| format | Article |
| id | doaj.art-0338660a18044a1b95afbca151230f39 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T13:15:28Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0338660a18044a1b95afbca151230f392022-12-21T17:45:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-10-01810.3389/fpls.2017.01793294704Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA AccumulationEmma Fernández-Crespo0Jose A. Navarro1Marta Serra-Soriano2Iván Finiti3Pilar García-Agustín4Vicente Pallás5Carmen González-Bosch6Grupo de Bioquímica y Biotecnología, Área de Fisiología Vegetal, Universitat Jaume I, Castellon de la Plana, SpainInstituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Valencia, SpainInstituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Valencia, SpainDepartament de Bioquímica, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Universitat de València, Valencia, SpainGrupo de Bioquímica y Biotecnología, Área de Fisiología Vegetal, Universitat Jaume I, Castellon de la Plana, SpainInstituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Valencia, SpainDepartament de Bioquímica, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Universitat de València, Valencia, SpainUnlike fungal and bacterial diseases, no direct method is available to control viral diseases. The use of resistance-inducing compounds can be an alternative strategy for plant viruses. Here we studied the basal response of melon to Melon necrotic spot virus (MNSV) and demonstrated the efficacy of hexanoic acid (Hx) priming, which prevents the virus from systemically spreading. We analysed callose deposition and the hormonal profile and gene expression at the whole plant level. This allowed us to determine hormonal homeostasis in the melon roots, cotyledons, hypocotyls, stems and leaves involved in basal and hexanoic acid-induced resistance (Hx-IR) to MNSV. Our data indicate important roles of salicylic acid (SA), 12-oxo-phytodienoic acid (OPDA), jasmonic-isoleucine, and ferulic acid in both responses to MNSV. The hormonal and metabolites balance, depending on the time and location associated with basal and Hx-IR, demonstrated the reprogramming of plant metabolism in MNSV-inoculated plants. The treatment with both SA and OPDA prior to virus infection significantly reduced MNSV systemic movement by inducing callose deposition. This demonstrates their relevance in Hx-IR against MNSV and a high correlation with callose deposition. Our data also provide valuable evidence to unravel priming mechanisms by natural compounds.http://journal.frontiersin.org/article/10.3389/fpls.2017.01793/fullMNSVCucumis melopriming by natural compoundshexanoic acidOPDAsalicylic acid |
| spellingShingle | Emma Fernández-Crespo Jose A. Navarro Marta Serra-Soriano Iván Finiti Pilar García-Agustín Vicente Pallás Carmen González-Bosch Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation Frontiers in Plant Science MNSV Cucumis melo priming by natural compounds hexanoic acid OPDA salicylic acid |
| title | Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation |
| title_full | Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation |
| title_fullStr | Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation |
| title_full_unstemmed | Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation |
| title_short | Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation |
| title_sort | hexanoic acid treatment prevents systemic mnsv movement in cucumis melo plants by priming callose deposition correlating sa and opda accumulation |
| topic | MNSV Cucumis melo priming by natural compounds hexanoic acid OPDA salicylic acid |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01793/full |
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