Shape effect of cerium oxide nanoparticles on mild traumatic brain injury
Abstract The catalytic performance and therapeutic effect of nanoparticles varies with shape. Here, we investigated and compared the therapeutic outcomes of ceria nanospheres (Ceria NSs) and ceria nanorods (Ceria NRs) in an in vivo study of mild traumatic brain injury (mTBI). In vivo TBI was induced...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-95057-9 |
| _version_ | 1818347131133493248 |
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| author | Dong Hyuk Youn Ngoc Minh Tran Bong Jun Kim Youngmi Kim Jin Pyeong Jeon Hyojong Yoo |
| author_facet | Dong Hyuk Youn Ngoc Minh Tran Bong Jun Kim Youngmi Kim Jin Pyeong Jeon Hyojong Yoo |
| author_sort | Dong Hyuk Youn |
| collection | DOAJ |
| description | Abstract The catalytic performance and therapeutic effect of nanoparticles varies with shape. Here, we investigated and compared the therapeutic outcomes of ceria nanospheres (Ceria NSs) and ceria nanorods (Ceria NRs) in an in vivo study of mild traumatic brain injury (mTBI). In vivo TBI was induced in a mouse model of open head injury using a stereotaxic impactor. Outcomes including cytoprotective effects, cognitive function, and cerebral edema were investigated after retro-orbital injection of 11.6 mM of ceria nanoparticles. Ceria nanoparticles significantly reduced fluoro-jade B (FJB)-positive cells and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive cells, and restored mRNA levels of superoxide dismutase 1 (SOD1) and SOD2. They also decreased the cyclooxygenase-2 (COX-2) expression compared with the untreated control group. Comparing the two nanomaterials, Ceria NRs showed less stable and high-energy (100) and (110) planes, which increased the number of active sites. The Ce3+/Ce4+ molar ratio of Ceria NRs (0.40) was greater than that of Ceria NSs (0.27). Ceria NRs (0.059 ± 0.021) appeared to exhibit better anti-inflammatory effect than Ceria NSs (0.133 ± 0.024), but the effect was statistically insignificant (p = 0.190). Ceria nanoparticles also improved cognitive impairment following mTBI compared with the control group, but the effect did not differ significantly according to the nanoshape. However, Ceria NRs (70.1 ± 0.5%) significantly decreased brain water content compared with Ceria NSs (73.7 ± 0.4%; p = 0.0015), indicating a more effective reduction in brain edema (p = 0.0015). Compared with Ceria NSs, the Ceria NRs are more effective in alleviating cerebral edema following in vivo mTBI. |
| first_indexed | 2024-12-13T17:29:17Z |
| format | Article |
| id | doaj.art-03395ed1143c4606955c5d68fef02b7e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T17:29:17Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-03395ed1143c4606955c5d68fef02b7e2022-12-21T23:37:06ZengNature PortfolioScientific Reports2045-23222021-07-011111810.1038/s41598-021-95057-9Shape effect of cerium oxide nanoparticles on mild traumatic brain injuryDong Hyuk Youn0Ngoc Minh Tran1Bong Jun Kim2Youngmi Kim3Jin Pyeong Jeon4Hyojong Yoo5Institute of New Frontier Research, Hallym University College of MedicineDepartment of Materials Science and Chemical Engineering, Hanyang UniversityInstitute of New Frontier Research, Hallym University College of MedicineInstitute of New Frontier Research, Hallym University College of MedicineInstitute of New Frontier Research, Hallym University College of MedicineDepartment of Materials Science and Chemical Engineering, Hanyang UniversityAbstract The catalytic performance and therapeutic effect of nanoparticles varies with shape. Here, we investigated and compared the therapeutic outcomes of ceria nanospheres (Ceria NSs) and ceria nanorods (Ceria NRs) in an in vivo study of mild traumatic brain injury (mTBI). In vivo TBI was induced in a mouse model of open head injury using a stereotaxic impactor. Outcomes including cytoprotective effects, cognitive function, and cerebral edema were investigated after retro-orbital injection of 11.6 mM of ceria nanoparticles. Ceria nanoparticles significantly reduced fluoro-jade B (FJB)-positive cells and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive cells, and restored mRNA levels of superoxide dismutase 1 (SOD1) and SOD2. They also decreased the cyclooxygenase-2 (COX-2) expression compared with the untreated control group. Comparing the two nanomaterials, Ceria NRs showed less stable and high-energy (100) and (110) planes, which increased the number of active sites. The Ce3+/Ce4+ molar ratio of Ceria NRs (0.40) was greater than that of Ceria NSs (0.27). Ceria NRs (0.059 ± 0.021) appeared to exhibit better anti-inflammatory effect than Ceria NSs (0.133 ± 0.024), but the effect was statistically insignificant (p = 0.190). Ceria nanoparticles also improved cognitive impairment following mTBI compared with the control group, but the effect did not differ significantly according to the nanoshape. However, Ceria NRs (70.1 ± 0.5%) significantly decreased brain water content compared with Ceria NSs (73.7 ± 0.4%; p = 0.0015), indicating a more effective reduction in brain edema (p = 0.0015). Compared with Ceria NSs, the Ceria NRs are more effective in alleviating cerebral edema following in vivo mTBI.https://doi.org/10.1038/s41598-021-95057-9 |
| spellingShingle | Dong Hyuk Youn Ngoc Minh Tran Bong Jun Kim Youngmi Kim Jin Pyeong Jeon Hyojong Yoo Shape effect of cerium oxide nanoparticles on mild traumatic brain injury Scientific Reports |
| title | Shape effect of cerium oxide nanoparticles on mild traumatic brain injury |
| title_full | Shape effect of cerium oxide nanoparticles on mild traumatic brain injury |
| title_fullStr | Shape effect of cerium oxide nanoparticles on mild traumatic brain injury |
| title_full_unstemmed | Shape effect of cerium oxide nanoparticles on mild traumatic brain injury |
| title_short | Shape effect of cerium oxide nanoparticles on mild traumatic brain injury |
| title_sort | shape effect of cerium oxide nanoparticles on mild traumatic brain injury |
| url | https://doi.org/10.1038/s41598-021-95057-9 |
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