Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy
An innovative methodology based on non-destructive observation by using harmonic generation microscopy is proposed for detection and location of starch granules and oil in a fried starchy matrix and topography analysis of food products. Specific fluorescent probes were used to label the main biochem...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-04-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/19/9/2024 |
| _version_ | 1798043535063121920 |
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| author | Agathe Chouët Sylvie Chevallier Romain Fleurisson Catherine Loisel Laurence Dubreil |
| author_facet | Agathe Chouët Sylvie Chevallier Romain Fleurisson Catherine Loisel Laurence Dubreil |
| author_sort | Agathe Chouët |
| collection | DOAJ |
| description | An innovative methodology based on non-destructive observation by using harmonic generation microscopy is proposed for detection and location of starch granules and oil in a fried starchy matrix and topography analysis of food products. Specific fluorescent probes were used to label the main biochemical components of the starchy fried matrix, namely starch and oil. Fluorescence of starch and oil respectively stained with Safranin O and Nile red was observed from non-linear microscopy. By using sequential scanning and specific emission filters, it was possible to merge fluorescence and harmonic generation signals. Second harmonic generation (SHG) generated by starch granules was superposed with safranin fluorescence, whereas third harmonic generation (THG), not restricted to the superposition with Nile red fluorescent signal, was used to investigate the topography of the fried product. By these experiments, starch granule mapping and topography of the starchy fried product were obtained without any destructive preparation of the sample. This label-free approach using harmonic generation microscopy is a very promising methodology for microstructure investigation of a large panel of starchy food products. |
| first_indexed | 2024-04-11T22:50:24Z |
| format | Article |
| id | doaj.art-033d33addd074051a42ebbf9c85be976 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:50:24Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-033d33addd074051a42ebbf9c85be9762022-12-22T03:58:35ZengMDPI AGSensors1424-82202019-04-01199202410.3390/s19092024s19092024Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation MicroscopyAgathe Chouët0Sylvie Chevallier1Romain Fleurisson2Catherine Loisel3Laurence Dubreil4Oniris, Univ Nantes, CNRS, GEPEA, UMR 6144, F-44000 Nantes, FranceOniris, Univ Nantes, CNRS, GEPEA, UMR 6144, F-44000 Nantes, FrancePAnTher, Oniris, INRA, Université Bretagne Loire, F-44307 Nantes, FranceOniris, Univ Nantes, CNRS, GEPEA, UMR 6144, F-44000 Nantes, FrancePAnTher, Oniris, INRA, Université Bretagne Loire, F-44307 Nantes, FranceAn innovative methodology based on non-destructive observation by using harmonic generation microscopy is proposed for detection and location of starch granules and oil in a fried starchy matrix and topography analysis of food products. Specific fluorescent probes were used to label the main biochemical components of the starchy fried matrix, namely starch and oil. Fluorescence of starch and oil respectively stained with Safranin O and Nile red was observed from non-linear microscopy. By using sequential scanning and specific emission filters, it was possible to merge fluorescence and harmonic generation signals. Second harmonic generation (SHG) generated by starch granules was superposed with safranin fluorescence, whereas third harmonic generation (THG), not restricted to the superposition with Nile red fluorescent signal, was used to investigate the topography of the fried product. By these experiments, starch granule mapping and topography of the starchy fried product were obtained without any destructive preparation of the sample. This label-free approach using harmonic generation microscopy is a very promising methodology for microstructure investigation of a large panel of starchy food products.https://www.mdpi.com/1424-8220/19/9/2024multiphoton microscopystarch granuletopographyharmonic generationSHGTHG |
| spellingShingle | Agathe Chouët Sylvie Chevallier Romain Fleurisson Catherine Loisel Laurence Dubreil Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy Sensors multiphoton microscopy starch granule topography harmonic generation SHG THG |
| title | Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy |
| title_full | Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy |
| title_fullStr | Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy |
| title_full_unstemmed | Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy |
| title_short | Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy |
| title_sort | label free fried starchy matrix investigation by harmonic generation microscopy |
| topic | multiphoton microscopy starch granule topography harmonic generation SHG THG |
| url | https://www.mdpi.com/1424-8220/19/9/2024 |
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