Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa)
A study was carried out to find out the favourable culture media for mass multiplication and the effect of carbon sources and duration on chlamydospore germination of Ustilaginoidea virens (Cook.) Tak. Different synthetic and semi synthetic solid, liquid media, substrate media were evaluated for ma...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2015-07-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/50113 |
| _version_ | 1828717883616657408 |
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| author | RITU RANI VINEET K SHARMA JAGJEET S LORE P P S PANNU |
| author_facet | RITU RANI VINEET K SHARMA JAGJEET S LORE P P S PANNU |
| author_sort | RITU RANI |
| collection | DOAJ |
| description |
A study was carried out to find out the favourable culture media for mass multiplication and the effect of carbon sources and duration on chlamydospore germination of Ustilaginoidea virens (Cook.) Tak. Different synthetic and semi synthetic solid, liquid media, substrate media were evaluated for mass multiplication of U. virens. Potato sucrose broth was the best medium for fast mycelia growth (104.80 mg) and sporulation (6.78 × 105 spores/ml), potato dextrose broth also favored mycelial growth, whereas corn meal dextrose broth was not suitable. Out of four solid media tested, maximum colony diameter (68.74 mm) and sporulation (6.86 × 105 spores/ml) was produced by potato sucrose agar followed by potato dextrose agar. Maximum sporulation was produced on substrate media based on barley seeds (5.54 × 105 spores/ml) followed by rice seeds (3.72 × 105 spores/ml) whereas rice husk based media produced minimum sporulation. Higher concentration of sucrose and dextrose at 2 per cent supported maximum germination of spores of U. virens and sucrose was found superior in inducing germination than dextrose. The results of this study help to understand the physiological and biochemical requirements for the growth and development of the pathogen, which could serve as an input in disease management to minimize the effect of false smut disease on rice.
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| first_indexed | 2024-03-12T14:34:52Z |
| format | Article |
| id | doaj.art-033ddba7926342a7b2a3a2d2287bfdf4 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-03-12T14:34:52Z |
| publishDate | 2015-07-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-033ddba7926342a7b2a3a2d2287bfdf42023-08-17T09:16:52ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192015-07-0185710.56093/ijas.v85i7.50113Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa)RITU RANI0VINEET K SHARMA1JAGJEET S LORE2P P S PANNU3Ph D Scholar, Punjab Agricultural University, Ludhiana 141 001Professor, Department of Plant Pathology, Punjab Agricultural University, Ludhiana 141 001Assistant Rice Pathologist, Punjab Agricultural University, Ludhiana 141 001Senior Plant Pathologist, Department of Plant Pathology, Punjab Agricultural University, Ludhiana 141 001 A study was carried out to find out the favourable culture media for mass multiplication and the effect of carbon sources and duration on chlamydospore germination of Ustilaginoidea virens (Cook.) Tak. Different synthetic and semi synthetic solid, liquid media, substrate media were evaluated for mass multiplication of U. virens. Potato sucrose broth was the best medium for fast mycelia growth (104.80 mg) and sporulation (6.78 × 105 spores/ml), potato dextrose broth also favored mycelial growth, whereas corn meal dextrose broth was not suitable. Out of four solid media tested, maximum colony diameter (68.74 mm) and sporulation (6.86 × 105 spores/ml) was produced by potato sucrose agar followed by potato dextrose agar. Maximum sporulation was produced on substrate media based on barley seeds (5.54 × 105 spores/ml) followed by rice seeds (3.72 × 105 spores/ml) whereas rice husk based media produced minimum sporulation. Higher concentration of sucrose and dextrose at 2 per cent supported maximum germination of spores of U. virens and sucrose was found superior in inducing germination than dextrose. The results of this study help to understand the physiological and biochemical requirements for the growth and development of the pathogen, which could serve as an input in disease management to minimize the effect of false smut disease on rice. https://epubs.icar.org.in/index.php/IJAgS/article/view/50113ColonyGrowthGerminationMediaSubstrateSporulation |
| spellingShingle | RITU RANI VINEET K SHARMA JAGJEET S LORE P P S PANNU Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa) The Indian Journal of Agricultural Sciences Colony Growth Germination Media Substrate Sporulation |
| title | Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa) |
| title_full | Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa) |
| title_fullStr | Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa) |
| title_full_unstemmed | Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa) |
| title_short | Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa) |
| title_sort | cultural studies on ustilaginoidea virens the incitant of false smut of rice oryza sativa |
| topic | Colony Growth Germination Media Substrate Sporulation |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/50113 |
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