Detection of seven Alternaria toxins in edible and medicinal herbs using ultra-high performance liquid chromatography-tandem mass spectrometry
Alternaria mycotoxins are ubiquitous mycotoxins that contaminate food and animal feed. Here, an UPLC-MS/MS was developed and used for the detection of seven Alternaria mycotoxins in 19 different edible and medicinal herbs. Extensive optimization resulted in a simple and convenient sample preparation...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157521000742 |
| _version_ | 1811311390557732864 |
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| author | Xiangsheng Zhao Dan Liu Xinquan Yang Lei Zhang Meihua Yang |
| author_facet | Xiangsheng Zhao Dan Liu Xinquan Yang Lei Zhang Meihua Yang |
| author_sort | Xiangsheng Zhao |
| collection | DOAJ |
| description | Alternaria mycotoxins are ubiquitous mycotoxins that contaminate food and animal feed. Here, an UPLC-MS/MS was developed and used for the detection of seven Alternaria mycotoxins in 19 different edible and medicinal herbs. Extensive optimization resulted in a simple and convenient sample preparation procedure with satisfactory extraction and a lower matrix effect. LOQs ranged from 0.01 to 2.0 ng/mL. Recoveries varied between 71.44% and 112.65%, with RSD less than 12%. The method was successfully applied for use in the mycotoxin analysis of 260 samples. A high percentage (28.46%) of samples were contaminated by 1–5 mycotoxins. Alternariol mono methylether was the predominant mycotoxin with high percentage of positive samples (37.5%), followed by alternariol (22.5%), alternariol (17.5%), tentoxin (10.83%), altertoxin Ⅰ (7.5%), and altenusin (4.17%). Collectively, the natural incidence data obtained from this study will help with better, validated risk assessments and efforts towards more comprehensive, future regulation. |
| first_indexed | 2024-04-13T10:17:01Z |
| format | Article |
| id | doaj.art-0340f511608443c189637beab37f1add |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-13T10:17:01Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-0340f511608443c189637beab37f1add2022-12-22T02:50:39ZengElsevierFood Chemistry: X2590-15752022-03-0113100186Detection of seven Alternaria toxins in edible and medicinal herbs using ultra-high performance liquid chromatography-tandem mass spectrometryXiangsheng Zhao0Dan Liu1Xinquan Yang2Lei Zhang3Meihua Yang4Key Laboratory of Resources Conservation and Development of Southern Medicine of Hainan Province & Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, China; Corresponding authors at: Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, NO. 4, Yaogu 4 Road, Xiuying District, Haikou, Hainan Province 570311, PR China (X. Zhao); Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151 Malianwa North Road, Haidian District, Beijing 100193, PR China (M. Yang).Key Laboratory of Resources Conservation and Development of Southern Medicine of Hainan Province & Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, ChinaKey Laboratory of Resources Conservation and Development of Southern Medicine of Hainan Province & Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaKey Laboratory of Resources Conservation and Development of Southern Medicine of Hainan Province & Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; Corresponding authors at: Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, NO. 4, Yaogu 4 Road, Xiuying District, Haikou, Hainan Province 570311, PR China (X. Zhao); Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151 Malianwa North Road, Haidian District, Beijing 100193, PR China (M. Yang).Alternaria mycotoxins are ubiquitous mycotoxins that contaminate food and animal feed. Here, an UPLC-MS/MS was developed and used for the detection of seven Alternaria mycotoxins in 19 different edible and medicinal herbs. Extensive optimization resulted in a simple and convenient sample preparation procedure with satisfactory extraction and a lower matrix effect. LOQs ranged from 0.01 to 2.0 ng/mL. Recoveries varied between 71.44% and 112.65%, with RSD less than 12%. The method was successfully applied for use in the mycotoxin analysis of 260 samples. A high percentage (28.46%) of samples were contaminated by 1–5 mycotoxins. Alternariol mono methylether was the predominant mycotoxin with high percentage of positive samples (37.5%), followed by alternariol (22.5%), alternariol (17.5%), tentoxin (10.83%), altertoxin Ⅰ (7.5%), and altenusin (4.17%). Collectively, the natural incidence data obtained from this study will help with better, validated risk assessments and efforts towards more comprehensive, future regulation.http://www.sciencedirect.com/science/article/pii/S2590157521000742AlternariaHerbsMycotoxinOccurrenceUPLC-MS/MSQuEChERS |
| spellingShingle | Xiangsheng Zhao Dan Liu Xinquan Yang Lei Zhang Meihua Yang Detection of seven Alternaria toxins in edible and medicinal herbs using ultra-high performance liquid chromatography-tandem mass spectrometry Food Chemistry: X Alternaria Herbs Mycotoxin Occurrence UPLC-MS/MS QuEChERS |
| title | Detection of seven Alternaria toxins in edible and medicinal herbs using ultra-high performance liquid chromatography-tandem mass spectrometry |
| title_full | Detection of seven Alternaria toxins in edible and medicinal herbs using ultra-high performance liquid chromatography-tandem mass spectrometry |
| title_fullStr | Detection of seven Alternaria toxins in edible and medicinal herbs using ultra-high performance liquid chromatography-tandem mass spectrometry |
| title_full_unstemmed | Detection of seven Alternaria toxins in edible and medicinal herbs using ultra-high performance liquid chromatography-tandem mass spectrometry |
| title_short | Detection of seven Alternaria toxins in edible and medicinal herbs using ultra-high performance liquid chromatography-tandem mass spectrometry |
| title_sort | detection of seven alternaria toxins in edible and medicinal herbs using ultra high performance liquid chromatography tandem mass spectrometry |
| topic | Alternaria Herbs Mycotoxin Occurrence UPLC-MS/MS QuEChERS |
| url | http://www.sciencedirect.com/science/article/pii/S2590157521000742 |
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