First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia
Background and Aim: Canine vector-borne diseases represent an important issue for the welfare and health of animals, but also have great zoonotic potential. These diseases are caused by bacteria, nematodes such as filariae, and other parasites such as Leishmania spp. Given the difficulty in differen...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-01-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.16/January-2023/11.pdf |
| _version_ | 1797954765066338304 |
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| author | Ruben Dario Pérez-Ramírez Rodrigo Lugo-Vargas Julieth Michel Petano-Duque Juan Sebastian Cruz-Méndez Iang Schroniltgen Rondón-Barragán |
| author_facet | Ruben Dario Pérez-Ramírez Rodrigo Lugo-Vargas Julieth Michel Petano-Duque Juan Sebastian Cruz-Méndez Iang Schroniltgen Rondón-Barragán |
| author_sort | Ruben Dario Pérez-Ramírez |
| collection | DOAJ |
| description | Background and Aim: Canine vector-borne diseases represent an important issue for the welfare and health of animals, but also have great zoonotic potential. These diseases are caused by bacteria, nematodes such as filariae, and other parasites such as Leishmania spp. Given the difficulty in differentiating common microfilariae in dogs by microscopy and serological methods, molecular techniques such as polymerase chain reaction (PCR) and sequencing should be valuable for reaching a reliable diagnosis. This study aimed to use microscopy and PCR to identify the microfilarial species in dogs from Valle del Cauca, Colombia, and a possible association with Leishmania infantum parasites.
Materials and Methods: This study was conducted on 270 dogs from Pradera and Florida municipalities. Microfilariae were detected in dogs by optical microscopy and amplification with 5.8S-ITS2-28S. Species identification was achieved through the amplification of the gene cytochrome oxidase I (COX1).
Results: Microscopic detection of microfilariae was possible in 4.81% (13/270) of the dogs. In addition, by PCR of COX1 and Sanger sequencing of ITS2, Acanthocheilonema reconditum was identified as the circulating microfilarial species in 12 dogs, coinfecting with the species L. infantum (Leishmania donovani complex).
Conclusion: To the best of our knowledge, this is the first report on A. reconditum and L. infantum mixed infection in dogs in Colombia, particularly in the Valle del Cauca. |
| first_indexed | 2024-04-10T23:23:43Z |
| format | Article |
| id | doaj.art-0342beb4ee6043f982de5ddb672c355e |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-10T23:23:43Z |
| publishDate | 2023-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-0342beb4ee6043f982de5ddb672c355e2023-01-12T13:06:31ZengVeterinary WorldVeterinary World0972-89882231-09162023-01-011619410310.14202/vetworld.2023.94-103First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest ColombiaRuben Dario Pérez-Ramírez0https://orcid.org/0000-0003-3163-9694Rodrigo Lugo-Vargas1https://orcid.org/0000-0001-8297-3364Julieth Michel Petano-Duque2https://orcid.org/0000-0002-7473-3472Juan Sebastian Cruz-Méndez3https://orcid.org/0000-0002-1048-012XIang Schroniltgen Rondón-Barragán4https://orcid.org/0000-0001-6980-892XResearch Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia.Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia.Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia.Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia.Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia.Background and Aim: Canine vector-borne diseases represent an important issue for the welfare and health of animals, but also have great zoonotic potential. These diseases are caused by bacteria, nematodes such as filariae, and other parasites such as Leishmania spp. Given the difficulty in differentiating common microfilariae in dogs by microscopy and serological methods, molecular techniques such as polymerase chain reaction (PCR) and sequencing should be valuable for reaching a reliable diagnosis. This study aimed to use microscopy and PCR to identify the microfilarial species in dogs from Valle del Cauca, Colombia, and a possible association with Leishmania infantum parasites. Materials and Methods: This study was conducted on 270 dogs from Pradera and Florida municipalities. Microfilariae were detected in dogs by optical microscopy and amplification with 5.8S-ITS2-28S. Species identification was achieved through the amplification of the gene cytochrome oxidase I (COX1). Results: Microscopic detection of microfilariae was possible in 4.81% (13/270) of the dogs. In addition, by PCR of COX1 and Sanger sequencing of ITS2, Acanthocheilonema reconditum was identified as the circulating microfilarial species in 12 dogs, coinfecting with the species L. infantum (Leishmania donovani complex). Conclusion: To the best of our knowledge, this is the first report on A. reconditum and L. infantum mixed infection in dogs in Colombia, particularly in the Valle del Cauca.http://www.veterinaryworld.org/Vol.16/January-2023/11.pdf5.8s-its2-28sacanthocheilonema reconditumcox1hsp70leishmania infantum |
| spellingShingle | Ruben Dario Pérez-Ramírez Rodrigo Lugo-Vargas Julieth Michel Petano-Duque Juan Sebastian Cruz-Méndez Iang Schroniltgen Rondón-Barragán First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia Veterinary World 5.8s-its2-28s acanthocheilonema reconditum cox1 hsp70 leishmania infantum |
| title | First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia |
| title_full | First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia |
| title_fullStr | First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia |
| title_full_unstemmed | First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia |
| title_short | First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia |
| title_sort | first study on microscopic and molecular detection of acanthocheilonema reconditum and leishmania infantum coinfection in dogs in southwest colombia |
| topic | 5.8s-its2-28s acanthocheilonema reconditum cox1 hsp70 leishmania infantum |
| url | http://www.veterinaryworld.org/Vol.16/January-2023/11.pdf |
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