Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina
Abstract Background Pennycress and camelina are two important novel biofuel oilseed crop species. Their seeds contain high content of oil that can be easily converted into biodiesel or jet fuel, while the left-over materials are usually made into press cake meals for feeding livestock. Therefore, th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-12-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13007-023-01114-x |
| _version_ | 1797397921862254592 |
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| author | Xin Li Victoria Yell Xu Li |
| author_facet | Xin Li Victoria Yell Xu Li |
| author_sort | Xin Li |
| collection | DOAJ |
| description | Abstract Background Pennycress and camelina are two important novel biofuel oilseed crop species. Their seeds contain high content of oil that can be easily converted into biodiesel or jet fuel, while the left-over materials are usually made into press cake meals for feeding livestock. Therefore, the ability to manipulate the seed coat encapsulating the oil- and protein-rich embryos is critical for improving seed oil production and press cake quality. Results Here, we tested the promoter activity of two Arabidopsis seed coat genes, AtTT10 and AtDP1, in pennycress and camelina by using eGFP and GUS reporters. Overall, both promoters show high levels of activities in the seed coat in these two biofuel crops, with very low or no expression in other tissues. Importantly, AtTT10 promoter activity in camelina shows differences from that in Arabidopsis, which highlights that the behavior of an exogenous promoter in closely related species cannot be assumed the same and still requires experimental determination. Conclusion Our work demonstrates that AtTT10 and AtDP1 promoters are suitable for driving gene expression in the outer integument of the seed coat in pennycress and camelina. |
| first_indexed | 2024-03-09T01:18:10Z |
| format | Article |
| id | doaj.art-0343fabb18454f5d843f3173b4208759 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-03-09T01:18:10Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-0343fabb18454f5d843f3173b42087592023-12-10T12:20:24ZengBMCPlant Methods1746-48112023-12-0119111010.1186/s13007-023-01114-xTwo Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelinaXin Li0Victoria Yell1Xu Li2Department of Plant and Microbial Biology, North Carolina State UniversityDepartment of Plant and Microbial Biology, North Carolina State UniversityDepartment of Plant and Microbial Biology, North Carolina State UniversityAbstract Background Pennycress and camelina are two important novel biofuel oilseed crop species. Their seeds contain high content of oil that can be easily converted into biodiesel or jet fuel, while the left-over materials are usually made into press cake meals for feeding livestock. Therefore, the ability to manipulate the seed coat encapsulating the oil- and protein-rich embryos is critical for improving seed oil production and press cake quality. Results Here, we tested the promoter activity of two Arabidopsis seed coat genes, AtTT10 and AtDP1, in pennycress and camelina by using eGFP and GUS reporters. Overall, both promoters show high levels of activities in the seed coat in these two biofuel crops, with very low or no expression in other tissues. Importantly, AtTT10 promoter activity in camelina shows differences from that in Arabidopsis, which highlights that the behavior of an exogenous promoter in closely related species cannot be assumed the same and still requires experimental determination. Conclusion Our work demonstrates that AtTT10 and AtDP1 promoters are suitable for driving gene expression in the outer integument of the seed coat in pennycress and camelina.https://doi.org/10.1186/s13007-023-01114-xSeed coatPromoterPennycressCamelinaBiofuel |
| spellingShingle | Xin Li Victoria Yell Xu Li Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina Plant Methods Seed coat Promoter Pennycress Camelina Biofuel |
| title | Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina |
| title_full | Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina |
| title_fullStr | Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina |
| title_full_unstemmed | Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina |
| title_short | Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina |
| title_sort | two arabidopsis promoters drive seed coat specific gene expression in pennycress and camelina |
| topic | Seed coat Promoter Pennycress Camelina Biofuel |
| url | https://doi.org/10.1186/s13007-023-01114-x |
| work_keys_str_mv | AT xinli twoarabidopsispromotersdriveseedcoatspecificgeneexpressioninpennycressandcamelina AT victoriayell twoarabidopsispromotersdriveseedcoatspecificgeneexpressioninpennycressandcamelina AT xuli twoarabidopsispromotersdriveseedcoatspecificgeneexpressioninpennycressandcamelina |