Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil
Cryptosporidium spp. is a protozoan that infects a wide range of vertebrate hosts; it has been reported to be the cause of severe illness or death in livestock worldwide, which leads to decreased performance and production losses, especially in young animals. This study investigated the presence of...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2020-08-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38147 |
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| author | Thábata dos Anjos Pacheco Felippe Danyel Cardoso Martins Sayanne Luns Hatum de Almeida Thiago Borges Fernandes Semedo Michelle Igarashi Watanabe Valéria Dutra João Luis Garcia Richard Campos Pacheco |
| author_facet | Thábata dos Anjos Pacheco Felippe Danyel Cardoso Martins Sayanne Luns Hatum de Almeida Thiago Borges Fernandes Semedo Michelle Igarashi Watanabe Valéria Dutra João Luis Garcia Richard Campos Pacheco |
| author_sort | Thábata dos Anjos Pacheco |
| collection | DOAJ |
| description | Cryptosporidium spp. is a protozoan that infects a wide range of vertebrate hosts; it has been reported to be the cause of severe illness or death in livestock worldwide, which leads to decreased performance and production losses, especially in young animals. This study investigated the presence of Cryptosporidium in calves from beef farms in the state of Mato Grosso, midwestern Brazil. For this purpose, fecal samples from 237 animals aged ? 45 days, raised in 20 rural properties were subjected to DNA extraction and nested polymerase chain reaction (nPCR) targeting 18S ribosomal RNA (18S rRNA) gene followed by sequencing. Additionally, positive samples, previously identified as Cryptosporidium parvum by sequencing and phylogenetic analyses based on 18S rRNA gene, were subsequently analyzed focusing the amplification and sequencing using nPCR of a fragment of the 60 kDa glycoprotein (gp60) gene. Of the 237 fecal samples analyzed by PCR (18S rRNA), 50 (21.1%) fecal samples were positive for Cryptosporidium spp., while 14 (70%) of the 20 properties had at least one positive animal. The following Cryptosporidium species were detected: C. bovis, C. parvum, and C. ryanae. Thereafter, two potentially zoonotic subtypes (IIaA15G2R1 and IIaA16G3R1) of C. parvum were identified based on gp60 gene sequences. This study resulted in the detection of subtype IIaA16G3R1 for the first time in South America and showed a wide distribution of the protozoan in beef farms in the studied area of the State. |
| first_indexed | 2024-04-10T20:36:44Z |
| format | Article |
| id | doaj.art-0346c2c6da454ba193e28d43368403f0 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:36:44Z |
| publishDate | 2020-08-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-0346c2c6da454ba193e28d43368403f02023-01-24T20:30:20ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592020-08-01415supl110.5433/1679-0359.2020v41n5supl1p2437Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, BrazilThábata dos Anjos Pacheco0Felippe Danyel Cardoso Martins1Sayanne Luns Hatum de Almeida2Thiago Borges Fernandes Semedo3Michelle Igarashi Watanabe4Valéria Dutra5João Luis Garcia6Richard Campos Pacheco7Universidade Federal do Mato GrossoUniversidade Estadual de LondrinaUniversidade Federal do Mato GrossoInstituto Nacional de Pesquisa do PantanalUniversidade Federal do Mato GrossoUniversidade Federal do Mato GrossoUniversidade Estadual de LondrinaUniversidade Federal do Mato GrossoCryptosporidium spp. is a protozoan that infects a wide range of vertebrate hosts; it has been reported to be the cause of severe illness or death in livestock worldwide, which leads to decreased performance and production losses, especially in young animals. This study investigated the presence of Cryptosporidium in calves from beef farms in the state of Mato Grosso, midwestern Brazil. For this purpose, fecal samples from 237 animals aged ? 45 days, raised in 20 rural properties were subjected to DNA extraction and nested polymerase chain reaction (nPCR) targeting 18S ribosomal RNA (18S rRNA) gene followed by sequencing. Additionally, positive samples, previously identified as Cryptosporidium parvum by sequencing and phylogenetic analyses based on 18S rRNA gene, were subsequently analyzed focusing the amplification and sequencing using nPCR of a fragment of the 60 kDa glycoprotein (gp60) gene. Of the 237 fecal samples analyzed by PCR (18S rRNA), 50 (21.1%) fecal samples were positive for Cryptosporidium spp., while 14 (70%) of the 20 properties had at least one positive animal. The following Cryptosporidium species were detected: C. bovis, C. parvum, and C. ryanae. Thereafter, two potentially zoonotic subtypes (IIaA15G2R1 and IIaA16G3R1) of C. parvum were identified based on gp60 gene sequences. This study resulted in the detection of subtype IIaA16G3R1 for the first time in South America and showed a wide distribution of the protozoan in beef farms in the studied area of the State.https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38147CalfCryptosporidium parvumFecesZoonosis. |
| spellingShingle | Thábata dos Anjos Pacheco Felippe Danyel Cardoso Martins Sayanne Luns Hatum de Almeida Thiago Borges Fernandes Semedo Michelle Igarashi Watanabe Valéria Dutra João Luis Garcia Richard Campos Pacheco Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil Semina: Ciências Agrárias Calf Cryptosporidium parvum Feces Zoonosis. |
| title | Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil |
| title_full | Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil |
| title_fullStr | Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil |
| title_full_unstemmed | Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil |
| title_short | Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil |
| title_sort | molecular survey of cryptosporidium spp in calves from the state of mato grosso brazil |
| topic | Calf Cryptosporidium parvum Feces Zoonosis. |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38147 |
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