Comparison of microRNA expression profiles of athletes involved in endurance sports and non-athletic volunteers using a hypoxia signaling pathway panel
In recent years, interest in the search for new potential molecular biomarkers to assess the effectiveness of the training process in elite sports, various pathologies caused by overload, and abuse of prohibited substances has reached an unprecedented level. MicroRNAs that regulate the processes of...
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| Format: | Article |
| Language: | English |
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National Electronic Information Consortium (NEICON)
2022-11-01
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| Series: | Спортивная медицина: наука и практика |
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| Online Access: | https://www.smjournal.ru/jour/article/view/422 |
| _version_ | 1797372482095677440 |
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| author | I. V. Pronina P. V. Postnikov V. I. Pavlov Z. G. Ordzhonikidze |
| author_facet | I. V. Pronina P. V. Postnikov V. I. Pavlov Z. G. Ordzhonikidze |
| author_sort | I. V. Pronina |
| collection | DOAJ |
| description | In recent years, interest in the search for new potential molecular biomarkers to assess the effectiveness of the training process in elite sports, various pathologies caused by overload, and abuse of prohibited substances has reached an unprecedented level. MicroRNAs that regulate the processes of metabolism, differentiation, proliferation, and apoptosis of cells at the post-transcriptional level are the most valuable candidates for use in this regard. They are found in all biological fluids of the human body, are stable during long-term storage and resistant to changes in environmental conditions. It is known that changes in the miRNA expression profile are closely associated with physical activity, as well as with the administration of recombinant hormones, erythropoiesis-stimulating agents, and other substances and methods that improve sports performance, which are prohibited by the World Anti-Doping Agency (WADA).Objective: In this study, plasma samples from athletes involved in endurance sports (race walking) and non-athletic volunteers were analyzed using a hypoxia signaling pathway panel to identify hypoxia candidate markers.Materials and methods: Expression profiles of plasma circulating miRNAs were assessed by reverse transcription followed by real-time quantitative polymerase chain reaction (RT Q-PCR). The obtained data were subjected to statistical processing using the CFX Manager Software v3.1 program.Results: Three potential microRNA markers were identified: hsa-miR-210-3p, hsa-miR-320a and hsa-miR-935 (increased in athletes by 61.6 times, 51.8 and 41.0 times, respectively) — of physiological response to the training, load, which may be associated with the emergence of hypoxia during endurance training.Conclusion: We have obtained preliminary data on differences in the expression profiles of circulating miRNAs in healthy non-athletic volunteers and professional athletes in the out-of-competition period. In the future, it is planned to expand the sample of studied blood plasma samples and compare the expression profiles of circulating microRNAs in athletes in competition and out-of-competition periods, as well as to compare the expression profiles of circulating microRNAs during aerobic and anaerobic loads. In addition, it is of interest to analyze changes in the expression levels of circulating miRNAs when using hypoxia mimetics applied to improve sports performance. |
| first_indexed | 2024-03-08T18:36:23Z |
| format | Article |
| id | doaj.art-0347793f51134d4785e7f3918d0805dd |
| institution | Directory Open Access Journal |
| issn | 2223-2524 2587-9014 |
| language | English |
| last_indexed | 2024-03-08T18:36:23Z |
| publishDate | 2022-11-01 |
| publisher | National Electronic Information Consortium (NEICON) |
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| series | Спортивная медицина: наука и практика |
| spelling | doaj.art-0347793f51134d4785e7f3918d0805dd2023-12-29T11:27:48ZengNational Electronic Information Consortium (NEICON)Спортивная медицина: наука и практика2223-25242587-90142022-11-01122132110.47529/2223-2524.2022.2.10296Comparison of microRNA expression profiles of athletes involved in endurance sports and non-athletic volunteers using a hypoxia signaling pathway panelI. V. Pronina0P. V. Postnikov1V. I. Pavlov2Z. G. Ordzhonikidze3National Anti-Doping Laboratory (Institute) of Lomonosov Moscow State University; Institute of General Pathology and PathophysiologyNational Anti-Doping Laboratory (Institute) of Lomonosov Moscow State UniversityMoscow Centre for Research and Practice in Medical Rehabilitation, Restorative and Sports Medicine of Moscow Healthcare DepartamentMoscow Centre for Research and Practice in Medical Rehabilitation, Restorative and Sports Medicine of Moscow Healthcare DepartamentIn recent years, interest in the search for new potential molecular biomarkers to assess the effectiveness of the training process in elite sports, various pathologies caused by overload, and abuse of prohibited substances has reached an unprecedented level. MicroRNAs that regulate the processes of metabolism, differentiation, proliferation, and apoptosis of cells at the post-transcriptional level are the most valuable candidates for use in this regard. They are found in all biological fluids of the human body, are stable during long-term storage and resistant to changes in environmental conditions. It is known that changes in the miRNA expression profile are closely associated with physical activity, as well as with the administration of recombinant hormones, erythropoiesis-stimulating agents, and other substances and methods that improve sports performance, which are prohibited by the World Anti-Doping Agency (WADA).Objective: In this study, plasma samples from athletes involved in endurance sports (race walking) and non-athletic volunteers were analyzed using a hypoxia signaling pathway panel to identify hypoxia candidate markers.Materials and methods: Expression profiles of plasma circulating miRNAs were assessed by reverse transcription followed by real-time quantitative polymerase chain reaction (RT Q-PCR). The obtained data were subjected to statistical processing using the CFX Manager Software v3.1 program.Results: Three potential microRNA markers were identified: hsa-miR-210-3p, hsa-miR-320a and hsa-miR-935 (increased in athletes by 61.6 times, 51.8 and 41.0 times, respectively) — of physiological response to the training, load, which may be associated with the emergence of hypoxia during endurance training.Conclusion: We have obtained preliminary data on differences in the expression profiles of circulating miRNAs in healthy non-athletic volunteers and professional athletes in the out-of-competition period. In the future, it is planned to expand the sample of studied blood plasma samples and compare the expression profiles of circulating microRNAs in athletes in competition and out-of-competition periods, as well as to compare the expression profiles of circulating microRNAs during aerobic and anaerobic loads. In addition, it is of interest to analyze changes in the expression levels of circulating miRNAs when using hypoxia mimetics applied to improve sports performance.https://www.smjournal.ru/jour/article/view/422circulating mirnasbiomarkersphysical activityefficiency of the training processendurancehypoxiadoping control |
| spellingShingle | I. V. Pronina P. V. Postnikov V. I. Pavlov Z. G. Ordzhonikidze Comparison of microRNA expression profiles of athletes involved in endurance sports and non-athletic volunteers using a hypoxia signaling pathway panel Спортивная медицина: наука и практика circulating mirnas biomarkers physical activity efficiency of the training process endurance hypoxia doping control |
| title | Comparison of microRNA expression profiles of athletes involved in endurance sports and non-athletic volunteers using a hypoxia signaling pathway panel |
| title_full | Comparison of microRNA expression profiles of athletes involved in endurance sports and non-athletic volunteers using a hypoxia signaling pathway panel |
| title_fullStr | Comparison of microRNA expression profiles of athletes involved in endurance sports and non-athletic volunteers using a hypoxia signaling pathway panel |
| title_full_unstemmed | Comparison of microRNA expression profiles of athletes involved in endurance sports and non-athletic volunteers using a hypoxia signaling pathway panel |
| title_short | Comparison of microRNA expression profiles of athletes involved in endurance sports and non-athletic volunteers using a hypoxia signaling pathway panel |
| title_sort | comparison of microrna expression profiles of athletes involved in endurance sports and non athletic volunteers using a hypoxia signaling pathway panel |
| topic | circulating mirnas biomarkers physical activity efficiency of the training process endurance hypoxia doping control |
| url | https://www.smjournal.ru/jour/article/view/422 |
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