The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl
For the first time, new possibilities of using the DPPH<sup>•</sup> as a signal-forming oxidant molecule with potentiometric detection are shown. The CV method confirmed the presence of a quasi-reversible potential-determining system DPPH<sup>•</sup>/DPPH-H under experimental...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1974 |
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| author | Elena Gerasimova Elena Gazizullina Sofya Kolbaczkaya Alla Ivanova |
| author_facet | Elena Gerasimova Elena Gazizullina Sofya Kolbaczkaya Alla Ivanova |
| author_sort | Elena Gerasimova |
| collection | DOAJ |
| description | For the first time, new possibilities of using the DPPH<sup>•</sup> as a signal-forming oxidant molecule with potentiometric detection are shown. The CV method confirmed the presence of a quasi-reversible potential-determining system DPPH<sup>•</sup>/DPPH-H under experimental conditions. This fact makes it possible to use DPPH<sup>•</sup> as the model of the oxidizing agent for obtaining an analytical signal by the potentiometry method. The potentiometric approach makes it possible to obtain the value of the Nernst slope and the antioxidant capacity in one experiment. It consists of an antioxidant supplement and two consecutive DPPH<sup>•</sup> supplements. In this case, the calculation of the Nernst slope is carried out by introducing the second addition of the oxidizing agent and constructing a calibration curve against the reaction background with an antioxidant. Solutions of individual antioxidants α-tocopherol, quercetin, (±)-catechin hydrate, and α-lipoic acid were studied by the developed approach. A high correlation with the results of spectrophotometric measurements is shown. At the same time, the potentiometry method is devoid of the concentration limitations of the spectrophotometric method, which was confirmed. In the study of plant materials extracts, a high correlation of antioxidant capacity, obtained by potentiometric and spectrophotometric methods, was shown only for objects whose color did not contribute to the DPPH<sup>•</sup> absorption. The versatility of the potentiometric method for studying objects of any color was shown. |
| first_indexed | 2024-03-09T20:48:12Z |
| format | Article |
| id | doaj.art-0349707aa0f34f7890fe21672ea19539 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:48:12Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-0349707aa0f34f7890fe21672ea195392023-11-23T22:38:44ZengMDPI AGAntioxidants2076-39212022-10-011110197410.3390/antiox11101974The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazylElena Gerasimova0Elena Gazizullina1Sofya Kolbaczkaya2Alla Ivanova3Analytical Chemistry Department, Institute of Chemical Engineering, Ural Federal University, 19 Mira Str., 620002 Ekaterinburg, RussiaAnalytical Chemistry Department, Institute of Chemical Engineering, Ural Federal University, 19 Mira Str., 620002 Ekaterinburg, RussiaAnalytical Chemistry Department, Institute of Chemical Engineering, Ural Federal University, 19 Mira Str., 620002 Ekaterinburg, RussiaAnalytical Chemistry Department, Institute of Chemical Engineering, Ural Federal University, 19 Mira Str., 620002 Ekaterinburg, RussiaFor the first time, new possibilities of using the DPPH<sup>•</sup> as a signal-forming oxidant molecule with potentiometric detection are shown. The CV method confirmed the presence of a quasi-reversible potential-determining system DPPH<sup>•</sup>/DPPH-H under experimental conditions. This fact makes it possible to use DPPH<sup>•</sup> as the model of the oxidizing agent for obtaining an analytical signal by the potentiometry method. The potentiometric approach makes it possible to obtain the value of the Nernst slope and the antioxidant capacity in one experiment. It consists of an antioxidant supplement and two consecutive DPPH<sup>•</sup> supplements. In this case, the calculation of the Nernst slope is carried out by introducing the second addition of the oxidizing agent and constructing a calibration curve against the reaction background with an antioxidant. Solutions of individual antioxidants α-tocopherol, quercetin, (±)-catechin hydrate, and α-lipoic acid were studied by the developed approach. A high correlation with the results of spectrophotometric measurements is shown. At the same time, the potentiometry method is devoid of the concentration limitations of the spectrophotometric method, which was confirmed. In the study of plant materials extracts, a high correlation of antioxidant capacity, obtained by potentiometric and spectrophotometric methods, was shown only for objects whose color did not contribute to the DPPH<sup>•</sup> absorption. The versatility of the potentiometric method for studying objects of any color was shown.https://www.mdpi.com/2076-3921/11/10/19742,2′-diphenyl-1-picrylhydrazylpotentiometryantioxidant capacity |
| spellingShingle | Elena Gerasimova Elena Gazizullina Sofya Kolbaczkaya Alla Ivanova The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl Antioxidants 2,2′-diphenyl-1-picrylhydrazyl potentiometry antioxidant capacity |
| title | The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl |
| title_full | The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl |
| title_fullStr | The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl |
| title_full_unstemmed | The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl |
| title_short | The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl |
| title_sort | novel potentiometric approach to antioxidant capacity assay based on the reaction with stable radical 2 2 diphenyl 1 picrylhydrazyl |
| topic | 2,2′-diphenyl-1-picrylhydrazyl potentiometry antioxidant capacity |
| url | https://www.mdpi.com/2076-3921/11/10/1974 |
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