Meristem genes are essential for the vegetative reproduction of Kalanchoë pinnata
Several Kalanchoë species reproduce asexually by forming plantlets in the leaf crenulations. Some species produce plantlets incessantly via somatic embryogenesis and organogenesis, whereas others exclusively develop plantlets after leaf detachment, presumably through organogenesis. SHOOT MERISTEMLES...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1157619/full |
| _version_ | 1797831659356160000 |
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| author | Francisco Jácome-Blásquez Minsung Kim |
| author_facet | Francisco Jácome-Blásquez Minsung Kim |
| author_sort | Francisco Jácome-Blásquez |
| collection | DOAJ |
| description | Several Kalanchoë species reproduce asexually by forming plantlets in the leaf crenulations. Some species produce plantlets incessantly via somatic embryogenesis and organogenesis, whereas others exclusively develop plantlets after leaf detachment, presumably through organogenesis. SHOOT MERISTEMLESS (STM), which mediates SAM functions, appears to be involved in Kalanchoë plantlet formation, suggesting that meristem genes may be essential for plantlet formation. However, the genetic regulatory network for establishing and maintaining plantlet primordia in Kalanchoë remains elusive. Here, we showed that meristem genes were differentially expressed in the leaf crenulations of K. pinnata during plantlet development after leaf detachment. The regulatory interactions among these meristem genes are largely conserved in K. pinnata crenulations. Moreover, transgenic antisense (AS) plants with lower expression of these key meristem genes formed significantly fewer plantlets with some morphological defects, suggesting that the meristem genes play an important role in plantlet formation and development. Our research revealed that key meristem genetic pathways were co-opted to the leaf margin to facilitate the unique asexual reproduction mechanism in K. pinnata. This also highlights how evolutionary tinkering invents new structures such as epiphyllous buds and plantlets by rewiring pre-existing genetic pathways. |
| first_indexed | 2024-04-09T13:56:17Z |
| format | Article |
| id | doaj.art-034e5fd5335d404aa64ae9736494652c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T13:56:17Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-034e5fd5335d404aa64ae9736494652c2023-05-08T04:52:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-05-011410.3389/fpls.2023.11576191157619Meristem genes are essential for the vegetative reproduction of Kalanchoë pinnataFrancisco Jácome-BlásquezMinsung KimSeveral Kalanchoë species reproduce asexually by forming plantlets in the leaf crenulations. Some species produce plantlets incessantly via somatic embryogenesis and organogenesis, whereas others exclusively develop plantlets after leaf detachment, presumably through organogenesis. SHOOT MERISTEMLESS (STM), which mediates SAM functions, appears to be involved in Kalanchoë plantlet formation, suggesting that meristem genes may be essential for plantlet formation. However, the genetic regulatory network for establishing and maintaining plantlet primordia in Kalanchoë remains elusive. Here, we showed that meristem genes were differentially expressed in the leaf crenulations of K. pinnata during plantlet development after leaf detachment. The regulatory interactions among these meristem genes are largely conserved in K. pinnata crenulations. Moreover, transgenic antisense (AS) plants with lower expression of these key meristem genes formed significantly fewer plantlets with some morphological defects, suggesting that the meristem genes play an important role in plantlet formation and development. Our research revealed that key meristem genetic pathways were co-opted to the leaf margin to facilitate the unique asexual reproduction mechanism in K. pinnata. This also highlights how evolutionary tinkering invents new structures such as epiphyllous buds and plantlets by rewiring pre-existing genetic pathways.https://www.frontiersin.org/articles/10.3389/fpls.2023.1157619/fullasexual and vegetative reproductionleaf crenulationsplantlet formationSHOOTMERISTEMLESS (STM)WUSCHELstem cells |
| spellingShingle | Francisco Jácome-Blásquez Minsung Kim Meristem genes are essential for the vegetative reproduction of Kalanchoë pinnata Frontiers in Plant Science asexual and vegetative reproduction leaf crenulations plantlet formation SHOOTMERISTEMLESS (STM) WUSCHEL stem cells |
| title | Meristem genes are essential for the vegetative reproduction of Kalanchoë pinnata |
| title_full | Meristem genes are essential for the vegetative reproduction of Kalanchoë pinnata |
| title_fullStr | Meristem genes are essential for the vegetative reproduction of Kalanchoë pinnata |
| title_full_unstemmed | Meristem genes are essential for the vegetative reproduction of Kalanchoë pinnata |
| title_short | Meristem genes are essential for the vegetative reproduction of Kalanchoë pinnata |
| title_sort | meristem genes are essential for the vegetative reproduction of kalanchoe pinnata |
| topic | asexual and vegetative reproduction leaf crenulations plantlet formation SHOOTMERISTEMLESS (STM) WUSCHEL stem cells |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1157619/full |
| work_keys_str_mv | AT franciscojacomeblasquez meristemgenesareessentialforthevegetativereproductionofkalanchoepinnata AT minsungkim meristemgenesareessentialforthevegetativereproductionofkalanchoepinnata |