| Summary: | To explore the phylogenetic relationships of the subfamily Centrotinae from the mitochondrial genome data, four complete mitogenomes (<i>Anchon lineatus</i>, <i>Anchon yunnanensis</i>, <i>Gargara genistae</i> and <i>Tricentrus longivalvulatus</i>) were sequenced and analyzed. All the newly sequenced mitogenomes contain 37 genes. Among the 13 protein-coding genes (PCGs) of the Centrotinae mitogenomes, a sliding window analysis and the ratio of Ka/Ks suggest that <i>atp8</i> is a relatively fast evolving gene, while <i>cox1</i> is the slowest. All PCGs start with ATN, except for <i>nad5</i> (start with TTG), and stop with TAA or the incomplete stop codon T, except for <i>nad2</i> and <i>cytb</i> (terminate with TAG). All tRNAs can fold into the typical cloverleaf secondary structure, except for <i>trnS1</i>, which lacks the dihydrouridine (DHU) arm. The BI and ML phylogenetic analyses of concatenated alignments of 13 mitochondrial PCGs among the major lineages produce a well-resolved framework. Phylogenetic analyses show that Membracoidea, Smiliinae and Centrotinae, together with tribes Centrotypini and Leptobelini are recovered as well-supported monophyletic groups. The tribe Gargarini (sensu Wallace et al.) and its monophyly are supported.
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