Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads
The bottleneck for the production of biofuels from microalgae consists on costly harvesting processes and low lipid production, immobilization technology could play a part on making the production of biofuels more feasible. The aim of this study was to evaluate the effect of alginate immobilization...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2023-01-01
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| Series: | JPhys Energy |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2515-7655/acb383 |
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| author | Daniel A Alfaro-Sayes Jerome Amoah Nova Rachmadona Shinji Hama Tomohisa Hasunuma Akihiko Kondo Chiaki Ogino |
| author_facet | Daniel A Alfaro-Sayes Jerome Amoah Nova Rachmadona Shinji Hama Tomohisa Hasunuma Akihiko Kondo Chiaki Ogino |
| author_sort | Daniel A Alfaro-Sayes |
| collection | DOAJ |
| description | The bottleneck for the production of biofuels from microalgae consists on costly harvesting processes and low lipid production, immobilization technology could play a part on making the production of biofuels more feasible. The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana , so far, the main focus of immobilization technology has been its use for wastewater treatment and nutrient removal from effluents. The microalgae Chlorella sorokiniana was cultured in both free and immobilized forms under optimal autotrophic growth conditions. Microalgae were immobilized in calcium alginate beads generated by mixing algal cells with a sodium alginate solution, followed by extrusion into a CaCl _2 solution. The results obtained in this study showed that the growth of the microalgae immobilized in alginate beads, was enhanced and achieved a dry cell weight 1.4-fold higher than that of a free cell culture, a higher light transmittance was also achieved in the alginate immobilized culture, and the lipid productivity was increased from 54.21 ± 2.48 mg l ^−1 d in the free cell culture to 82.22 ± 8.48 mg l ^−1 d in the immobilized culture. These results demonstrate the effectiveness of immobilization technology for promoting growth and lipid productivity in the microalgae Chlorella sorokiniana . |
| first_indexed | 2024-04-09T17:25:32Z |
| format | Article |
| id | doaj.art-0353008bd4a04111866c8cb4cf79dedd |
| institution | Directory Open Access Journal |
| issn | 2515-7655 |
| language | English |
| last_indexed | 2024-04-09T17:25:32Z |
| publishDate | 2023-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | JPhys Energy |
| spelling | doaj.art-0353008bd4a04111866c8cb4cf79dedd2023-04-18T13:49:40ZengIOP PublishingJPhys Energy2515-76552023-01-015101401910.1088/2515-7655/acb383Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beadsDaniel A Alfaro-Sayes0https://orcid.org/0000-0002-4034-3505Jerome Amoah1Nova Rachmadona2Shinji Hama3Tomohisa Hasunuma4Akihiko Kondo5Chiaki Ogino6https://orcid.org/0000-0002-8906-7724Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University , Kobe, JapanGraduate School of Science, Technology and Innovation, Kobe University , Kobe, JapanDepartment of Chemical Science and Engineering, Graduate School of Engineering, Kobe University , Kobe, Japan; Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran , Jatinangor, West Java 45363, Indonesia; Research Collaboration Center for Biomass and Biorefinery between BRIN and Universitas Padjadjaran , Jatinangor, West Java 45363, IndonesiaResearch and Development Laboratory, Bio-energy Corporation , Amagasaki, JapanGraduate School of Science, Technology and Innovation, Kobe University , Kobe, JapanDepartment of Chemical Science and Engineering, Graduate School of Engineering, Kobe University , Kobe, Japan; Graduate School of Science, Technology and Innovation, Kobe University , Kobe, JapanDepartment of Chemical Science and Engineering, Graduate School of Engineering, Kobe University , Kobe, JapanThe bottleneck for the production of biofuels from microalgae consists on costly harvesting processes and low lipid production, immobilization technology could play a part on making the production of biofuels more feasible. The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana , so far, the main focus of immobilization technology has been its use for wastewater treatment and nutrient removal from effluents. The microalgae Chlorella sorokiniana was cultured in both free and immobilized forms under optimal autotrophic growth conditions. Microalgae were immobilized in calcium alginate beads generated by mixing algal cells with a sodium alginate solution, followed by extrusion into a CaCl _2 solution. The results obtained in this study showed that the growth of the microalgae immobilized in alginate beads, was enhanced and achieved a dry cell weight 1.4-fold higher than that of a free cell culture, a higher light transmittance was also achieved in the alginate immobilized culture, and the lipid productivity was increased from 54.21 ± 2.48 mg l ^−1 d in the free cell culture to 82.22 ± 8.48 mg l ^−1 d in the immobilized culture. These results demonstrate the effectiveness of immobilization technology for promoting growth and lipid productivity in the microalgae Chlorella sorokiniana .https://doi.org/10.1088/2515-7655/acb383Chlorella sorokinianabiomasslipidsalginateimmobilized cell |
| spellingShingle | Daniel A Alfaro-Sayes Jerome Amoah Nova Rachmadona Shinji Hama Tomohisa Hasunuma Akihiko Kondo Chiaki Ogino Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads JPhys Energy Chlorella sorokiniana biomass lipids alginate immobilized cell |
| title | Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads |
| title_full | Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads |
| title_fullStr | Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads |
| title_full_unstemmed | Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads |
| title_short | Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads |
| title_sort | enhanced growth and lipid productivity by living chlorella sorokiniana immobilized in ca alginate beads |
| topic | Chlorella sorokiniana biomass lipids alginate immobilized cell |
| url | https://doi.org/10.1088/2515-7655/acb383 |
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