Toward Improving Human Islet Isolation from Younger Donors: Rescue Purification is Efficient for Trapped Islets
Several reports suggest that islets isolated from younger donor pancreata are of better quality for clinical islet transplantation. The relative inefficiency of the continuous gradient purification process (CGP) is one of the major obstacles to the utilization of these younger donor pancreata. This...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2009-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368909788237159 |
| _version_ | 1819088556092555264 |
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| author | A. Miki C. Ricordi S. Messinger T. Yamamoto A. Mita S. Barker R. Haetter A. Khan R. Alejandro H. Ichii M.D., Ph.D. |
| author_facet | A. Miki C. Ricordi S. Messinger T. Yamamoto A. Mita S. Barker R. Haetter A. Khan R. Alejandro H. Ichii M.D., Ph.D. |
| author_sort | A. Miki |
| collection | DOAJ |
| description | Several reports suggest that islets isolated from younger donor pancreata are of better quality for clinical islet transplantation. The relative inefficiency of the continuous gradient purification process (CGP) is one of the major obstacles to the utilization of these younger donor pancreata. This study demonstrates the benefits of utilizing an additional purification step, rescue gradient purification (RGP), to recover trapped islets and examines the possible superiority of these rescued islets. Seventy-three human islet isolations purified by RGP following CGP were divided into two groups based on age, and the isolation results were retrospectively analyzed (group I: age ≤40, group II: age >40). The quality of islets from both CGP and RGP were assessed by β-cell fractional viability (βFV) and ADP/ATP ratio. Significant increases in the percent islet recovery from RGP and the percent trapped islets in group I compared to group II were observed. Donor age correlated negatively to the percent islets recovered from RGP ( R = 0.440) and to the percent of trapped islets ( R = 0.511). RGP islets had higher βFV and better ADP/ATP ratio compared to CGP islets. In conclusion, RGP improved the efficiency in the purification of trapped islets, which often come from younger donor pancreata. The better quality of β-cells in RGP islets encourages us to perform RGP, considering the higher quality as well as the quantity of remaining islets. |
| first_indexed | 2024-12-21T21:53:55Z |
| format | Article |
| id | doaj.art-035467c3880a4393a7c212915de0cc65 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-21T21:53:55Z |
| publishDate | 2009-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-035467c3880a4393a7c212915de0cc652022-12-21T18:49:00ZengSAGE PublishingCell Transplantation0963-68971555-38922009-01-011810.3727/096368909788237159Toward Improving Human Islet Isolation from Younger Donors: Rescue Purification is Efficient for Trapped IsletsA. Miki0C. Ricordi1S. Messinger2T. Yamamoto3A. Mita4S. Barker5R. Haetter6A. Khan7R. Alejandro8H. Ichii M.D., Ph.D.9Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USAJackson Memorial Hospital Transplant Institute, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USADepartment of Epidemiology and Public Health, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USACell Transplant Center, Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USACell Transplant Center, Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USACell Transplant Center, Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USACell Transplant Center, Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USACell Transplant Center, Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USADepartment of Medicine, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USAJackson Memorial Hospital Transplant Institute, University of Miami Leonard M. Miller School of Medicine, Miami, FL, USASeveral reports suggest that islets isolated from younger donor pancreata are of better quality for clinical islet transplantation. The relative inefficiency of the continuous gradient purification process (CGP) is one of the major obstacles to the utilization of these younger donor pancreata. This study demonstrates the benefits of utilizing an additional purification step, rescue gradient purification (RGP), to recover trapped islets and examines the possible superiority of these rescued islets. Seventy-three human islet isolations purified by RGP following CGP were divided into two groups based on age, and the isolation results were retrospectively analyzed (group I: age ≤40, group II: age >40). The quality of islets from both CGP and RGP were assessed by β-cell fractional viability (βFV) and ADP/ATP ratio. Significant increases in the percent islet recovery from RGP and the percent trapped islets in group I compared to group II were observed. Donor age correlated negatively to the percent islets recovered from RGP ( R = 0.440) and to the percent of trapped islets ( R = 0.511). RGP islets had higher βFV and better ADP/ATP ratio compared to CGP islets. In conclusion, RGP improved the efficiency in the purification of trapped islets, which often come from younger donor pancreata. The better quality of β-cells in RGP islets encourages us to perform RGP, considering the higher quality as well as the quantity of remaining islets.https://doi.org/10.3727/096368909788237159 |
| spellingShingle | A. Miki C. Ricordi S. Messinger T. Yamamoto A. Mita S. Barker R. Haetter A. Khan R. Alejandro H. Ichii M.D., Ph.D. Toward Improving Human Islet Isolation from Younger Donors: Rescue Purification is Efficient for Trapped Islets Cell Transplantation |
| title | Toward Improving Human Islet Isolation from Younger Donors: Rescue Purification is Efficient for Trapped Islets |
| title_full | Toward Improving Human Islet Isolation from Younger Donors: Rescue Purification is Efficient for Trapped Islets |
| title_fullStr | Toward Improving Human Islet Isolation from Younger Donors: Rescue Purification is Efficient for Trapped Islets |
| title_full_unstemmed | Toward Improving Human Islet Isolation from Younger Donors: Rescue Purification is Efficient for Trapped Islets |
| title_short | Toward Improving Human Islet Isolation from Younger Donors: Rescue Purification is Efficient for Trapped Islets |
| title_sort | toward improving human islet isolation from younger donors rescue purification is efficient for trapped islets |
| url | https://doi.org/10.3727/096368909788237159 |
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