Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model
Objective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem histomorphologic analyses to have a practical refere...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-12-01
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| Series: | Current Therapeutic Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0011393X14000186 |
| _version_ | 1811340392782626816 |
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| author | Hyun-Jin Tae, DVM, PhD Natalia Petrashevskaya, PhD Ismayil Ahmet, PhD Sungha Park, MD, PhD Mark I. Talan, MD, PhD Edward G. Lakatta, MD Li Lin, PhD |
| author_facet | Hyun-Jin Tae, DVM, PhD Natalia Petrashevskaya, PhD Ismayil Ahmet, PhD Sungha Park, MD, PhD Mark I. Talan, MD, PhD Edward G. Lakatta, MD Li Lin, PhD |
| author_sort | Hyun-Jin Tae, DVM, PhD |
| collection | DOAJ |
| description | Objective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem histomorphologic analyses to have a practical reference for future clinical applications.
Methods: Vessel ultrasound sonography was performed in a sRAGE-treated rat carotid artery balloon injury model at different time points after the surgery, and therapeutic efficacy of different doses of sRAGE produced in Chinese hamster ovary cells and with different N-glycoform modifications were assessed.
Results: Vessel ultrasound sonography found that sRAGE produced in Chinese hamster ovary cells with complex N-glycoform modifications is highly effective, and is consistent with our recent findings in the same model assessed with histology. We also found that sonography is less sensitive than histology when a higher dose of sRAGE is administered.
Conclusions: Sonograph results are consistent with those obtained from histology; that is, sRAGE produced in Chinese hamster ovary cells has significantly higher efficacy than insect cell-originated sRAGE cells. |
| first_indexed | 2024-04-13T18:41:17Z |
| format | Article |
| id | doaj.art-0359438d9667429abed4046f9e846eba |
| institution | Directory Open Access Journal |
| issn | 0011-393X 1879-0313 |
| language | English |
| last_indexed | 2024-04-13T18:41:17Z |
| publishDate | 2014-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Therapeutic Research |
| spelling | doaj.art-0359438d9667429abed4046f9e846eba2022-12-22T02:34:42ZengElsevierCurrent Therapeutic Research0011-393X1879-03132014-12-0176C11011510.1016/j.curtheres.2014.08.001Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury ModelHyun-Jin Tae, DVM, PhD0Natalia Petrashevskaya, PhD1Ismayil Ahmet, PhD2Sungha Park, MD, PhD3Mark I. Talan, MD, PhD4Edward G. Lakatta, MD5Li Lin, PhD6Laboratory of Cardiovascular Science, National Institute on Aging, National Institutes of Health, Baltimore, MarylandLaboratory of Cardiovascular Science, National Institute on Aging, National Institutes of Health, Baltimore, MarylandLaboratory of Cardiovascular Science, National Institute on Aging, National Institutes of Health, Baltimore, MarylandDivision of Cardiology, Cardiovascular Center, Yonsei University College of Medicine, Seoul, KoreaLaboratory of Cardiovascular Science, National Institute on Aging, National Institutes of Health, Baltimore, MarylandLaboratory of Cardiovascular Science, National Institute on Aging, National Institutes of Health, Baltimore, MarylandLaboratory of Cardiovascular Science, National Institute on Aging, National Institutes of Health, Baltimore, MarylandObjective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem histomorphologic analyses to have a practical reference for future clinical applications. Methods: Vessel ultrasound sonography was performed in a sRAGE-treated rat carotid artery balloon injury model at different time points after the surgery, and therapeutic efficacy of different doses of sRAGE produced in Chinese hamster ovary cells and with different N-glycoform modifications were assessed. Results: Vessel ultrasound sonography found that sRAGE produced in Chinese hamster ovary cells with complex N-glycoform modifications is highly effective, and is consistent with our recent findings in the same model assessed with histology. We also found that sonography is less sensitive than histology when a higher dose of sRAGE is administered. Conclusions: Sonograph results are consistent with those obtained from histology; that is, sRAGE produced in Chinese hamster ovary cells has significantly higher efficacy than insect cell-originated sRAGE cells.http://www.sciencedirect.com/science/article/pii/S0011393X14000186neointimaN-glycosylationlsoluble receptor for advanced glycation end productsvascular injuryvessel sonography |
| spellingShingle | Hyun-Jin Tae, DVM, PhD Natalia Petrashevskaya, PhD Ismayil Ahmet, PhD Sungha Park, MD, PhD Mark I. Talan, MD, PhD Edward G. Lakatta, MD Li Lin, PhD Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model Current Therapeutic Research neointima N-glycosylationl soluble receptor for advanced glycation end products vascular injury vessel sonography |
| title | Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model |
| title_full | Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model |
| title_fullStr | Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model |
| title_full_unstemmed | Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model |
| title_short | Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model |
| title_sort | vessel ultrasound sonographic assessment of soluble receptor for advanced glycation end products efficacy in a rat balloon injury model |
| topic | neointima N-glycosylationl soluble receptor for advanced glycation end products vascular injury vessel sonography |
| url | http://www.sciencedirect.com/science/article/pii/S0011393X14000186 |
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