Overexpression of the mulberry latex gene MaMLX-Q1 enhances defense against Plutella xylostella in Arabidopsis thaliana
Purified mulberry latex chitinase (MLX) has a role in defense against some lepidopteran insects. In this study, a full length chitinase gene, MaMLX-Q1, of 1405 bp with a 1140 bp open reading frame, was obtained from mulberry leaves by the degenerate primers and rapid amplification of cDNA e...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2017-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600097L.pdf |
| Summary: | Purified mulberry latex chitinase (MLX) has a role in defense against some
lepidopteran insects. In this study, a full length chitinase gene, MaMLX-Q1,
of 1405 bp with a 1140 bp open reading frame, was obtained from mulberry
leaves by the degenerate primers and rapid amplification of cDNA ends
polymerase chain reaction (RACE-PCR) procedure. The gene encoded a mature
protein with the predicted molecular mass of 39.38 kDa and an isoelectric
point (pI) of 6.43; it contained two chitin-binding domains and a hydrolase
family 19 chitinase domain. Sequence alignment and phylogenetic analysis
grouped it in the class I chitinase protein group. Heterogeneous expression
of this MaMLX-Q1 in Arabidopsis showed non-visible alterations in growth
phenotype, except for the higher transcriptional expression of MaMLX-Q1 when
compared to that of wild-type Arabidopsis. This ectopic MaMLX-Q1 exhibited
toxicity to the growth and development of Plutella xylostella larvae, causing
significantly lower weight gain and higher mortality. These results indicate
an application of MaMLX-Q1 as an insecticide for plant protection. |
|---|---|
| ISSN: | 0354-4664 1821-4339 |