Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada)
Protists are key stone components of aquatic ecosystems, sustaining primary productivity and aquatic food webs. However, their diversity, ecology and structuring factors shaping their temporal distribution remain strongly misunderstood in freshwaters. Using high-throughput sequencing on water sample...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02359/full |
| _version_ | 1828393001084256256 |
|---|---|
| author | Perrine Cruaud Perrine Cruaud Perrine Cruaud Adrien Vigneron Adrien Vigneron Adrien Vigneron Marie-Stéphanie Fradette Marie-Stéphanie Fradette Marie-Stéphanie Fradette Caetano C. Dorea Alexander I. Culley Alexander I. Culley Alexander I. Culley Manuel J. Rodriguez Manuel J. Rodriguez Steve J. Charette Steve J. Charette Steve J. Charette |
| author_facet | Perrine Cruaud Perrine Cruaud Perrine Cruaud Adrien Vigneron Adrien Vigneron Adrien Vigneron Marie-Stéphanie Fradette Marie-Stéphanie Fradette Marie-Stéphanie Fradette Caetano C. Dorea Alexander I. Culley Alexander I. Culley Alexander I. Culley Manuel J. Rodriguez Manuel J. Rodriguez Steve J. Charette Steve J. Charette Steve J. Charette |
| author_sort | Perrine Cruaud |
| collection | DOAJ |
| description | Protists are key stone components of aquatic ecosystems, sustaining primary productivity and aquatic food webs. However, their diversity, ecology and structuring factors shaping their temporal distribution remain strongly misunderstood in freshwaters. Using high-throughput sequencing on water samples collected over 16 different months (including two summer and two winter periods), combined with geochemical measurements and climate monitoring, we comprehensively determined the pico- and nanoeukaryotic community composition and dynamics in a Canadian river undergoing prolonged ice-cover winters. Our analysis revealed a large protist diversity in this fluctuating ecosystem and clear seasonal patterns demonstrating a direct and/or indirect selective role of abiotic factors, such as water temperature or nitrogen concentrations, in structuring the eukaryotic microbial community. Nonetheless, our results also revealed that primary productivity, predatory as well as parasitism lifestyles, inferred from fine phylogenetic placements, remained potentially present over the annual cycle, despite the large seasonal fluctuations and the remodeling of the community composition under ice. In addition, potential interplays with the bacterial community composition were identified supporting a possible contribution of the bacterial community to the temporal dynamics of the protist community structure. Our results illustrate the complexity of the eukaryotic microbial community and provide a substantive and useful dataset to better understand the global freshwater ecosystem functioning. |
| first_indexed | 2024-12-10T07:31:16Z |
| format | Article |
| id | doaj.art-03638ed56db14cf39d3370565061ad54 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T07:31:16Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-03638ed56db14cf39d3370565061ad542022-12-22T01:57:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-10-011010.3389/fmicb.2019.02359465101Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada)Perrine Cruaud0Perrine Cruaud1Perrine Cruaud2Adrien Vigneron3Adrien Vigneron4Adrien Vigneron5Marie-Stéphanie Fradette6Marie-Stéphanie Fradette7Marie-Stéphanie Fradette8Caetano C. Dorea9Alexander I. Culley10Alexander I. Culley11Alexander I. Culley12Manuel J. Rodriguez13Manuel J. Rodriguez14Steve J. Charette15Steve J. Charette16Steve J. Charette17Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec City, QC, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec City, QC, CanadaCRAD, Université Laval, Québec City, QC, CanadaInstitut de Biologie Intégrative et des Systèmes, Université Laval, Québec City, QC, CanadaCentre D’Études Nordiques, Université Laval, Québec City, QC, CanadaDépartement de Biologie, Université Laval, Québec City, QC, CanadaInstitut de Biologie Intégrative et des Systèmes, Université Laval, Québec City, QC, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec City, QC, CanadaCRAD, Université Laval, Québec City, QC, CanadaDepartment of Civil Engineering, University of Victoria, Victoria, BC, CanadaInstitut de Biologie Intégrative et des Systèmes, Université Laval, Québec City, QC, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec City, QC, CanadaGroupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec City, QC, CanadaCRAD, Université Laval, Québec City, QC, CanadaÉcole Supérieure D’aménagement du Territoire et de Développement Régional (ESAD), Université Laval, Québec City, QC, CanadaInstitut de Biologie Intégrative et des Systèmes, Université Laval, Québec City, QC, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec City, QC, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, Québec City, QC, CanadaProtists are key stone components of aquatic ecosystems, sustaining primary productivity and aquatic food webs. However, their diversity, ecology and structuring factors shaping their temporal distribution remain strongly misunderstood in freshwaters. Using high-throughput sequencing on water samples collected over 16 different months (including two summer and two winter periods), combined with geochemical measurements and climate monitoring, we comprehensively determined the pico- and nanoeukaryotic community composition and dynamics in a Canadian river undergoing prolonged ice-cover winters. Our analysis revealed a large protist diversity in this fluctuating ecosystem and clear seasonal patterns demonstrating a direct and/or indirect selective role of abiotic factors, such as water temperature or nitrogen concentrations, in structuring the eukaryotic microbial community. Nonetheless, our results also revealed that primary productivity, predatory as well as parasitism lifestyles, inferred from fine phylogenetic placements, remained potentially present over the annual cycle, despite the large seasonal fluctuations and the remodeling of the community composition under ice. In addition, potential interplays with the bacterial community composition were identified supporting a possible contribution of the bacterial community to the temporal dynamics of the protist community structure. Our results illustrate the complexity of the eukaryotic microbial community and provide a substantive and useful dataset to better understand the global freshwater ecosystem functioning.https://www.frontiersin.org/article/10.3389/fmicb.2019.02359/fullmicrobial eukaryotesprotistsfreshwaterriverseasonal cycleswinter |
| spellingShingle | Perrine Cruaud Perrine Cruaud Perrine Cruaud Adrien Vigneron Adrien Vigneron Adrien Vigneron Marie-Stéphanie Fradette Marie-Stéphanie Fradette Marie-Stéphanie Fradette Caetano C. Dorea Alexander I. Culley Alexander I. Culley Alexander I. Culley Manuel J. Rodriguez Manuel J. Rodriguez Steve J. Charette Steve J. Charette Steve J. Charette Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada) Frontiers in Microbiology microbial eukaryotes protists freshwater river seasonal cycles winter |
| title | Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada) |
| title_full | Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada) |
| title_fullStr | Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada) |
| title_full_unstemmed | Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada) |
| title_short | Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada) |
| title_sort | annual protist community dynamics in a freshwater ecosystem undergoing contrasted climatic conditions the saint charles river canada |
| topic | microbial eukaryotes protists freshwater river seasonal cycles winter |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02359/full |
| work_keys_str_mv | AT perrinecruaud annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT perrinecruaud annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT perrinecruaud annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT adrienvigneron annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT adrienvigneron annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT adrienvigneron annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT mariestephaniefradette annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT mariestephaniefradette annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT mariestephaniefradette annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT caetanocdorea annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT alexandericulley annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT alexandericulley annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT alexandericulley annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT manueljrodriguez annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT manueljrodriguez annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT stevejcharette annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT stevejcharette annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada AT stevejcharette annualprotistcommunitydynamicsinafreshwaterecosystemundergoingcontrastedclimaticconditionsthesaintcharlesrivercanada |