Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos
Although phenotypic abnormalities frequently appear in the placenta following somatic cell nuclear transfer (SCNT), mouse trophoblast stem cells (TSCs) established from SCNT embryos reportedly show no distinct abnormalities compared with those derived from normal fertilization. In this study, we ree...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-07-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2018.1507199 |
| _version_ | 1827811513995362304 |
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| author | Michiko Hirose Masashi Hada Satoshi Kamimura Shogo Matoba Arata Honda Kaori Motomura Narumi Ogonuki Hossam H. Shawki Kimiko Inoue Satoru Takahashi Atsuo Ogura |
| author_facet | Michiko Hirose Masashi Hada Satoshi Kamimura Shogo Matoba Arata Honda Kaori Motomura Narumi Ogonuki Hossam H. Shawki Kimiko Inoue Satoru Takahashi Atsuo Ogura |
| author_sort | Michiko Hirose |
| collection | DOAJ |
| description | Although phenotypic abnormalities frequently appear in the placenta following somatic cell nuclear transfer (SCNT), mouse trophoblast stem cells (TSCs) established from SCNT embryos reportedly show no distinct abnormalities compared with those derived from normal fertilization. In this study, we reexamined SCNT–TSCs to identify their imprinting statuses. Placenta-specific maternally imprinted genes (Gab1, Slc38a4, and Sfmbt2) consistently showed biallelic expression in SCNT–TSCs, suggesting their loss of imprinting (LOI). The LOI of Gab1 was associated with decreased DNA methylation, and that of Sfmbt2 was associated with decreased DNA methylation and histone H3K27 trimethylation. The maternal allele of the intergenic differentially methylated region (IG–DMR) was aberrantly hypermethylated following SCNT, even though this region was prone to demethylation in TSCs when established in a serum-free chemically defined medium. These findings indicate that the development of cloned embryos is associated with imprinting abnormalities specifically in the trophoblast lineage from its initial stage, which may affect subsequent placental development. |
| first_indexed | 2024-03-11T23:06:06Z |
| format | Article |
| id | doaj.art-0364f2a3d53b42d4b303ec151d41a253 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:06:06Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-0364f2a3d53b42d4b303ec151d41a2532023-09-21T13:09:21ZengTaylor & Francis GroupEpigenetics1559-22941559-23082018-07-0113769370310.1080/15592294.2018.15071991507199Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryosMichiko Hirose0Masashi Hada1Satoshi Kamimura2Shogo Matoba3Arata Honda4Kaori Motomura5Narumi Ogonuki6Hossam H. Shawki7Kimiko Inoue8Satoru Takahashi9Atsuo Ogura10RIKEN BioResource Research CenterRIKEN BioResource Research CenterRIKEN BioResource Research CenterRIKEN BioResource Research CenterRIKEN BioResource Research CenterRIKEN BioResource Research CenterRIKEN BioResource Research CenterUniversity of TsukubaRIKEN BioResource Research CenterUniversity of TsukubaRIKEN BioResource Research CenterAlthough phenotypic abnormalities frequently appear in the placenta following somatic cell nuclear transfer (SCNT), mouse trophoblast stem cells (TSCs) established from SCNT embryos reportedly show no distinct abnormalities compared with those derived from normal fertilization. In this study, we reexamined SCNT–TSCs to identify their imprinting statuses. Placenta-specific maternally imprinted genes (Gab1, Slc38a4, and Sfmbt2) consistently showed biallelic expression in SCNT–TSCs, suggesting their loss of imprinting (LOI). The LOI of Gab1 was associated with decreased DNA methylation, and that of Sfmbt2 was associated with decreased DNA methylation and histone H3K27 trimethylation. The maternal allele of the intergenic differentially methylated region (IG–DMR) was aberrantly hypermethylated following SCNT, even though this region was prone to demethylation in TSCs when established in a serum-free chemically defined medium. These findings indicate that the development of cloned embryos is associated with imprinting abnormalities specifically in the trophoblast lineage from its initial stage, which may affect subsequent placental development.http://dx.doi.org/10.1080/15592294.2018.1507199nuclear transfermousetrophoblast stem cellig-dmrplacentaimprinting |
| spellingShingle | Michiko Hirose Masashi Hada Satoshi Kamimura Shogo Matoba Arata Honda Kaori Motomura Narumi Ogonuki Hossam H. Shawki Kimiko Inoue Satoru Takahashi Atsuo Ogura Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos Epigenetics nuclear transfer mouse trophoblast stem cell ig-dmr placenta imprinting |
| title | Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos |
| title_full | Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos |
| title_fullStr | Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos |
| title_full_unstemmed | Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos |
| title_short | Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos |
| title_sort | aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer derived embryos |
| topic | nuclear transfer mouse trophoblast stem cell ig-dmr placenta imprinting |
| url | http://dx.doi.org/10.1080/15592294.2018.1507199 |
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