On the way from SARS-CoV-sensitive mice to murine COVID-19 model
The coronavirus disease 2019 (COVID-19) is a master killer which appeared suddenly and which has already claimed more than 200,000 human lives. In this situation, laboratories are in urgent need for a COVID-19 murine model to search for effective antiviral compounds. Here we propose a novel strategy...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Belgorod National Research University
2020-05-01
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| Series: | Research Results in Pharmacology |
| Online Access: | https://rrpharmacology.pensoft.net/article/53633/download/pdf/ |
| _version_ | 1797429121802829824 |
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| author | Vladislav O. Soldatov Marina V. Kubekina Yulia Yu. Silaeva Alexandra V. Bruter Alexey V. Deykin |
| author_facet | Vladislav O. Soldatov Marina V. Kubekina Yulia Yu. Silaeva Alexandra V. Bruter Alexey V. Deykin |
| author_sort | Vladislav O. Soldatov |
| collection | DOAJ |
| description | The coronavirus disease 2019 (COVID-19) is a master killer which appeared suddenly and which has already claimed more than 200,000 human lives. In this situation, laboratories are in urgent need for a COVID-19 murine model to search for effective antiviral compounds. Here we propose a novel strategy for the development of mice that can be inoculated by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the COVID-19 causative agent. In humans, two proteins – ACE2 and TMPRSS2 – are involved in SARS-CoV-2 cells entry and, thus, we decided to introduce their genes into a murine genome. These genes will be placed with LoxP sites under the murine Tmprss2 promoter. Such an approach can provide a representative model with the opportunity to control the viral sensitivity of an animal population and tissue specificity of hACE2 and hTMPRSS2 expression. Graphical abstract The new COVID-19 model should be based on inducible co-expression of the human ACE2 and TMPRSS2 genes. Activation of ACE2 and TMPRSS2 genes will occur only in the virological laboratory, after crossbreeding with Cre-mice. Before activation, mice will be resistant to SARS-CoV-2 for their biological safety during the pandemic. |
| first_indexed | 2024-03-09T09:08:39Z |
| format | Article |
| id | doaj.art-0369c47572364b588c9ebb49491c8975 |
| institution | Directory Open Access Journal |
| issn | 2658-381X |
| language | English |
| last_indexed | 2024-03-09T09:08:39Z |
| publishDate | 2020-05-01 |
| publisher | Belgorod National Research University |
| record_format | Article |
| series | Research Results in Pharmacology |
| spelling | doaj.art-0369c47572364b588c9ebb49491c89752023-12-02T09:28:42ZengBelgorod National Research UniversityResearch Results in Pharmacology2658-381X2020-05-01621710.3897/rrpharmacology.6.5363353633On the way from SARS-CoV-sensitive mice to murine COVID-19 modelVladislav O. Soldatov0Marina V. Kubekina1Yulia Yu. Silaeva2Alexandra V. Bruter3Alexey V. Deykin4Belgorod State UniversityInstitute of Gene Biology of the Russian Academy of SciencesInstitute of Gene Biology of the Russian Academy of SciencesInstitute of Gene Biology of the Russian Academy of SciencesInstitute of General Pathology and Pathophysiology of the Russian Academy of Medical SciencesThe coronavirus disease 2019 (COVID-19) is a master killer which appeared suddenly and which has already claimed more than 200,000 human lives. In this situation, laboratories are in urgent need for a COVID-19 murine model to search for effective antiviral compounds. Here we propose a novel strategy for the development of mice that can be inoculated by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the COVID-19 causative agent. In humans, two proteins – ACE2 and TMPRSS2 – are involved in SARS-CoV-2 cells entry and, thus, we decided to introduce their genes into a murine genome. These genes will be placed with LoxP sites under the murine Tmprss2 promoter. Such an approach can provide a representative model with the opportunity to control the viral sensitivity of an animal population and tissue specificity of hACE2 and hTMPRSS2 expression. Graphical abstract The new COVID-19 model should be based on inducible co-expression of the human ACE2 and TMPRSS2 genes. Activation of ACE2 and TMPRSS2 genes will occur only in the virological laboratory, after crossbreeding with Cre-mice. Before activation, mice will be resistant to SARS-CoV-2 for their biological safety during the pandemic.https://rrpharmacology.pensoft.net/article/53633/download/pdf/ |
| spellingShingle | Vladislav O. Soldatov Marina V. Kubekina Yulia Yu. Silaeva Alexandra V. Bruter Alexey V. Deykin On the way from SARS-CoV-sensitive mice to murine COVID-19 model Research Results in Pharmacology |
| title | On the way from SARS-CoV-sensitive mice to murine COVID-19 model |
| title_full | On the way from SARS-CoV-sensitive mice to murine COVID-19 model |
| title_fullStr | On the way from SARS-CoV-sensitive mice to murine COVID-19 model |
| title_full_unstemmed | On the way from SARS-CoV-sensitive mice to murine COVID-19 model |
| title_short | On the way from SARS-CoV-sensitive mice to murine COVID-19 model |
| title_sort | on the way from sars cov sensitive mice to murine covid 19 model |
| url | https://rrpharmacology.pensoft.net/article/53633/download/pdf/ |
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