Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples
Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reserv...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/224 |
| _version_ | 1827598659316875264 |
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| author | Ravleen Virdi Melissa E. Lowe Grant J. Norton Stephanie N. Dawrs Nabeeh A. Hasan L. Elaine Epperson Cody M. Glickman Edward D. Chan Michael Strong James L. Crooks Jennifer R. Honda |
| author_facet | Ravleen Virdi Melissa E. Lowe Grant J. Norton Stephanie N. Dawrs Nabeeh A. Hasan L. Elaine Epperson Cody M. Glickman Edward D. Chan Michael Strong James L. Crooks Jennifer R. Honda |
| author_sort | Ravleen Virdi |
| collection | DOAJ |
| description | Nontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reservoirs led us to identify NTM from patient respiratory specimens and compare NTM diversity between outdoor and indoor locations in Hawai’i. A total of 545 water biofilm samples were collected from 357 unique locations across Kaua’i (<i>n</i> = 51), O’ahu (<i>n</i> = 202), Maui (<i>n</i> = 159), and Hawai’i Island (<i>n</i> = 133) and divided into outdoor (<i>n</i> = 179) or indoor (<i>n</i> = 366) categories. <i>rpoB</i> sequence analysis was used to determine NTM species and predictive modeling applied to develop NTM risk maps based on geographic characteristics between environments. <i>M. chimaera</i> was frequently identified from respiratory and environmental samples followed by <i>M. chelonae</i> and <i>M. abscessus;</i> yet significantly less NTM were consistently recovered from outdoor compared to indoor biofilms, as exemplified by showerhead biofilm samples. While the frequency of <i>M. chimaera</i> recovery was comparable between outdoor and indoor showerhead biofilms, phylogenetic analyses demonstrate similar <i>rpoB</i> gene sequences between all showerhead and respiratory <i>M. chimaera</i> isolates, supporting outdoor and indoor environments as possible sources for pulmonary <i>M. chimaera</i> infections. |
| first_indexed | 2024-03-09T03:57:08Z |
| format | Article |
| id | doaj.art-036bf958bfc3462898cd1d2f1246ac1c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T03:57:08Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-036bf958bfc3462898cd1d2f1246ac1c2023-12-03T14:18:06ZengMDPI AGMicroorganisms2076-26072021-01-019222410.3390/microorganisms9020224Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor SamplesRavleen Virdi0Melissa E. Lowe1Grant J. Norton2Stephanie N. Dawrs3Nabeeh A. Hasan4L. Elaine Epperson5Cody M. Glickman6Edward D. Chan7Michael Strong8James L. Crooks9Jennifer R. Honda10Center for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206, USADivision of Biostatistics and Bioinformatics, National Jewish Health, Denver, CO 80206, USACenter for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206, USACenter for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206, USACenter for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206, USACenter for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206, USAComputational Biosciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Medicine and Academic Affairs, National Jewish Health, Denver, CO 80206, USACenter for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206, USADivision of Biostatistics and Bioinformatics, National Jewish Health, Denver, CO 80206, USACenter for Genes, Environment, and Health, National Jewish Health, Denver, CO 80206, USANontuberculous mycobacteria (NTM) are environmental organisms that can cause opportunistic pulmonary disease with species diversity showing significant regional variation. In the United States, Hawai’i shows the highest rate of NTM pulmonary disease. The need for improved understanding of NTM reservoirs led us to identify NTM from patient respiratory specimens and compare NTM diversity between outdoor and indoor locations in Hawai’i. A total of 545 water biofilm samples were collected from 357 unique locations across Kaua’i (<i>n</i> = 51), O’ahu (<i>n</i> = 202), Maui (<i>n</i> = 159), and Hawai’i Island (<i>n</i> = 133) and divided into outdoor (<i>n</i> = 179) or indoor (<i>n</i> = 366) categories. <i>rpoB</i> sequence analysis was used to determine NTM species and predictive modeling applied to develop NTM risk maps based on geographic characteristics between environments. <i>M. chimaera</i> was frequently identified from respiratory and environmental samples followed by <i>M. chelonae</i> and <i>M. abscessus;</i> yet significantly less NTM were consistently recovered from outdoor compared to indoor biofilms, as exemplified by showerhead biofilm samples. While the frequency of <i>M. chimaera</i> recovery was comparable between outdoor and indoor showerhead biofilms, phylogenetic analyses demonstrate similar <i>rpoB</i> gene sequences between all showerhead and respiratory <i>M. chimaera</i> isolates, supporting outdoor and indoor environments as possible sources for pulmonary <i>M. chimaera</i> infections.https://www.mdpi.com/2076-2607/9/2/224nontuberculous mycobacteriabiofilmsenvironmentHawai’i |
| spellingShingle | Ravleen Virdi Melissa E. Lowe Grant J. Norton Stephanie N. Dawrs Nabeeh A. Hasan L. Elaine Epperson Cody M. Glickman Edward D. Chan Michael Strong James L. Crooks Jennifer R. Honda Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples Microorganisms nontuberculous mycobacteria biofilms environment Hawai’i |
| title | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
| title_full | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
| title_fullStr | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
| title_full_unstemmed | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
| title_short | Lower Recovery of Nontuberculous Mycobacteria from Outdoor Hawai’i Environmental Water Biofilms Compared to Indoor Samples |
| title_sort | lower recovery of nontuberculous mycobacteria from outdoor hawai i environmental water biofilms compared to indoor samples |
| topic | nontuberculous mycobacteria biofilms environment Hawai’i |
| url | https://www.mdpi.com/2076-2607/9/2/224 |
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