Characterisation of New <i>Foxunavirus</i> Phage Murka with the Potential of <i>Xanthomonas campestris</i> pv. <i>campestris</i> Control
Phages of phytopathogenic bacteria are considered to be promising agents for the biological control of bacterial diseases in plants. This paper reports on the isolation and characterisation of a new <i>Xanthomonas campestris</i> pv. <i>campestris</i> phage, Murka. Phage morph...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/2/198 |
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| author | Peter V. Evseev Rashit I. Tarakanov Ha T. N. Vo Natalia E. Suzina Anna A. Vasilyeva Alexander N. Ignatov Konstantin A. Miroshnikov Fevzi S.-U. Dzhalilov |
| author_facet | Peter V. Evseev Rashit I. Tarakanov Ha T. N. Vo Natalia E. Suzina Anna A. Vasilyeva Alexander N. Ignatov Konstantin A. Miroshnikov Fevzi S.-U. Dzhalilov |
| author_sort | Peter V. Evseev |
| collection | DOAJ |
| description | Phages of phytopathogenic bacteria are considered to be promising agents for the biological control of bacterial diseases in plants. This paper reports on the isolation and characterisation of a new <i>Xanthomonas campestris</i> pv. <i>campestris</i> phage, Murka. Phage morphology and basic kinetic characteristics of the infection were determined, and a phylogenomic analysis was performed. The phage was able to lyse a reasonably broad range (64%, 9 of the 14 of the <i>Xanthomonas campestris</i> pv. <i>campestris</i> strains used in the study) of circulating strains of the cabbage black rot pathogen. This lytic myovirus has a DNA genome of 44,044 bp and contains 83 predicted genes. Taxonomically, it belongs to the genus <i>Foxunavirus</i>. This bacteriophage is promising for use as a possible means of biological control of cabbage black rot. |
| first_indexed | 2024-03-07T22:10:42Z |
| format | Article |
| id | doaj.art-036c4e7cbcf24181af29f1914484bf9c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-07T22:10:42Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-036c4e7cbcf24181af29f1914484bf9c2024-02-23T15:37:27ZengMDPI AGViruses1999-49152024-01-0116219810.3390/v16020198Characterisation of New <i>Foxunavirus</i> Phage Murka with the Potential of <i>Xanthomonas campestris</i> pv. <i>campestris</i> ControlPeter V. Evseev0Rashit I. Tarakanov1Ha T. N. Vo2Natalia E. Suzina3Anna A. Vasilyeva4Alexander N. Ignatov5Konstantin A. Miroshnikov6Fevzi S.-U. Dzhalilov7Department of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, 127434 Moscow, RussiaDepartment of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, 127434 Moscow, RussiaFaculty of Agronomy, Nong Lam University, Quarter 6, Thu Duc District, Ho Chi Minh City 721400, VietnamSkryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences”, Prosp. Nauki, 5, 142290 Pushchino, RussiaDepartment of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, 127434 Moscow, RussiaDepartment of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, 127434 Moscow, RussiaDepartment of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, 127434 Moscow, RussiaDepartment of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya Str. 49, 127434 Moscow, RussiaPhages of phytopathogenic bacteria are considered to be promising agents for the biological control of bacterial diseases in plants. This paper reports on the isolation and characterisation of a new <i>Xanthomonas campestris</i> pv. <i>campestris</i> phage, Murka. Phage morphology and basic kinetic characteristics of the infection were determined, and a phylogenomic analysis was performed. The phage was able to lyse a reasonably broad range (64%, 9 of the 14 of the <i>Xanthomonas campestris</i> pv. <i>campestris</i> strains used in the study) of circulating strains of the cabbage black rot pathogen. This lytic myovirus has a DNA genome of 44,044 bp and contains 83 predicted genes. Taxonomically, it belongs to the genus <i>Foxunavirus</i>. This bacteriophage is promising for use as a possible means of biological control of cabbage black rot.https://www.mdpi.com/1999-4915/16/2/198<i>Xanthomonas</i> bacteriophagescabbage<i>Brassicae</i>biocontrolbacteriophage taxonomy |
| spellingShingle | Peter V. Evseev Rashit I. Tarakanov Ha T. N. Vo Natalia E. Suzina Anna A. Vasilyeva Alexander N. Ignatov Konstantin A. Miroshnikov Fevzi S.-U. Dzhalilov Characterisation of New <i>Foxunavirus</i> Phage Murka with the Potential of <i>Xanthomonas campestris</i> pv. <i>campestris</i> Control Viruses <i>Xanthomonas</i> bacteriophages cabbage <i>Brassicae</i> biocontrol bacteriophage taxonomy |
| title | Characterisation of New <i>Foxunavirus</i> Phage Murka with the Potential of <i>Xanthomonas campestris</i> pv. <i>campestris</i> Control |
| title_full | Characterisation of New <i>Foxunavirus</i> Phage Murka with the Potential of <i>Xanthomonas campestris</i> pv. <i>campestris</i> Control |
| title_fullStr | Characterisation of New <i>Foxunavirus</i> Phage Murka with the Potential of <i>Xanthomonas campestris</i> pv. <i>campestris</i> Control |
| title_full_unstemmed | Characterisation of New <i>Foxunavirus</i> Phage Murka with the Potential of <i>Xanthomonas campestris</i> pv. <i>campestris</i> Control |
| title_short | Characterisation of New <i>Foxunavirus</i> Phage Murka with the Potential of <i>Xanthomonas campestris</i> pv. <i>campestris</i> Control |
| title_sort | characterisation of new i foxunavirus i phage murka with the potential of i xanthomonas campestris i pv i campestris i control |
| topic | <i>Xanthomonas</i> bacteriophages cabbage <i>Brassicae</i> biocontrol bacteriophage taxonomy |
| url | https://www.mdpi.com/1999-4915/16/2/198 |
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