Phytochemical Profile, Safety Assessment and Wound Healing Activity of <i>Artemisia absinthium</i> L.
The aim of study was to validate, by in vitro and in vivo studies, the traditional use for wound-healing activity of <i>Artemisia absinthium</i> L. Reversed-phase liquid chromatography coupled with diode array detection and electrospray ion trap mass spectrometry (RP-LC-DAD-ESI-MS) analy...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/12/1744 |
| _version_ | 1797545144937873408 |
|---|---|
| author | Amel Boudjelal Antonella Smeriglio Giovanna Ginestra Marcella Denaro Domenico Trombetta |
| author_facet | Amel Boudjelal Antonella Smeriglio Giovanna Ginestra Marcella Denaro Domenico Trombetta |
| author_sort | Amel Boudjelal |
| collection | DOAJ |
| description | The aim of study was to validate, by in vitro and in vivo studies, the traditional use for wound-healing activity of <i>Artemisia absinthium</i> L. Reversed-phase liquid chromatography coupled with diode array detection and electrospray ion trap mass spectrometry (RP-LC-DAD-ESI-MS) analysis allowed to identify eleven polyphenols with chlorogenic acid as the most abundant compound (3.75 g/100 g of dry extract). After that, antibacterial activity as well as acute dermal and oral toxicity were assessed in animal models. In order to investigate the wound-healing activity of <i>A. absinthium</i> methanol extract, two ointments were formulated (MEO 5% and 10%). The ointment with the highest concentration of plant extract (10%) showed a statistically significant effect on the rats wound contraction, similar to that exerted by the reference drug Cicatryl-Bio. Moreover, <i>A. absinthium</i> methanol extract showed the best antibacterial activity against the Gram-negative <i>Escherichia coli</i> ATCC 10536 (MIC 1.25–2.5 mg/mL) and the Gram-positive <i>Staphylococcus aureus</i> ATCC 6538 (0.31–0.625 mg/mL). The absence of oral and topical toxicity of the treated animals allowed to establish the safety of the ointments. Overall, data collected in the present study support and validate the use of <i>A. absinthium</i> as a wound healing agent in the Algerian traditional medicine. |
| first_indexed | 2024-03-10T14:10:21Z |
| format | Article |
| id | doaj.art-036dc851993b486b827af0055e12aa5e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:10:21Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-036dc851993b486b827af0055e12aa5e2023-11-21T00:10:16ZengMDPI AGPlants2223-77472020-12-01912174410.3390/plants9121744Phytochemical Profile, Safety Assessment and Wound Healing Activity of <i>Artemisia absinthium</i> L.Amel Boudjelal0Antonella Smeriglio1Giovanna Ginestra2Marcella Denaro3Domenico Trombetta4Department of Microbiology and Biochemistry, Faculty of Sciences, University of M’sila, M’sila 28000, AlgeriaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Via Giovanni Palatucci, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Via Giovanni Palatucci, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Via Giovanni Palatucci, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Via Giovanni Palatucci, 98168 Messina, ItalyThe aim of study was to validate, by in vitro and in vivo studies, the traditional use for wound-healing activity of <i>Artemisia absinthium</i> L. Reversed-phase liquid chromatography coupled with diode array detection and electrospray ion trap mass spectrometry (RP-LC-DAD-ESI-MS) analysis allowed to identify eleven polyphenols with chlorogenic acid as the most abundant compound (3.75 g/100 g of dry extract). After that, antibacterial activity as well as acute dermal and oral toxicity were assessed in animal models. In order to investigate the wound-healing activity of <i>A. absinthium</i> methanol extract, two ointments were formulated (MEO 5% and 10%). The ointment with the highest concentration of plant extract (10%) showed a statistically significant effect on the rats wound contraction, similar to that exerted by the reference drug Cicatryl-Bio. Moreover, <i>A. absinthium</i> methanol extract showed the best antibacterial activity against the Gram-negative <i>Escherichia coli</i> ATCC 10536 (MIC 1.25–2.5 mg/mL) and the Gram-positive <i>Staphylococcus aureus</i> ATCC 6538 (0.31–0.625 mg/mL). The absence of oral and topical toxicity of the treated animals allowed to establish the safety of the ointments. Overall, data collected in the present study support and validate the use of <i>A. absinthium</i> as a wound healing agent in the Algerian traditional medicine.https://www.mdpi.com/2223-7747/9/12/1744<i>Artemisia absinthium</i> L.polyphenolswound healing activityantibacterial activityacute dermal toxicityacute oral toxicity |
| spellingShingle | Amel Boudjelal Antonella Smeriglio Giovanna Ginestra Marcella Denaro Domenico Trombetta Phytochemical Profile, Safety Assessment and Wound Healing Activity of <i>Artemisia absinthium</i> L. Plants <i>Artemisia absinthium</i> L. polyphenols wound healing activity antibacterial activity acute dermal toxicity acute oral toxicity |
| title | Phytochemical Profile, Safety Assessment and Wound Healing Activity of <i>Artemisia absinthium</i> L. |
| title_full | Phytochemical Profile, Safety Assessment and Wound Healing Activity of <i>Artemisia absinthium</i> L. |
| title_fullStr | Phytochemical Profile, Safety Assessment and Wound Healing Activity of <i>Artemisia absinthium</i> L. |
| title_full_unstemmed | Phytochemical Profile, Safety Assessment and Wound Healing Activity of <i>Artemisia absinthium</i> L. |
| title_short | Phytochemical Profile, Safety Assessment and Wound Healing Activity of <i>Artemisia absinthium</i> L. |
| title_sort | phytochemical profile safety assessment and wound healing activity of i artemisia absinthium i l |
| topic | <i>Artemisia absinthium</i> L. polyphenols wound healing activity antibacterial activity acute dermal toxicity acute oral toxicity |
| url | https://www.mdpi.com/2223-7747/9/12/1744 |
| work_keys_str_mv | AT amelboudjelal phytochemicalprofilesafetyassessmentandwoundhealingactivityofiartemisiaabsinthiumil AT antonellasmeriglio phytochemicalprofilesafetyassessmentandwoundhealingactivityofiartemisiaabsinthiumil AT giovannaginestra phytochemicalprofilesafetyassessmentandwoundhealingactivityofiartemisiaabsinthiumil AT marcelladenaro phytochemicalprofilesafetyassessmentandwoundhealingactivityofiartemisiaabsinthiumil AT domenicotrombetta phytochemicalprofilesafetyassessmentandwoundhealingactivityofiartemisiaabsinthiumil |