Effect of storage conditions on the formation of type A and B trichothecenes in cereal products
Introduction and objective Mycotoxins are toxic metabolites produced by certain types of fungi, causing pathological changes in humans and animals. The aim of this study was to assess the degree of contamination of selected cereal grains, bran and cereal products intended for children, with mycotoxi...
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| Format: | Article |
| Language: | English |
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Institute of Rural Health
2019-06-01
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| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/aaem/The-effect-of-storage-conditions-on-the-formation-of-type-A-and-B-trichothecenes,103398,0,2.html |
| _version_ | 1818535910095978496 |
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| author | Piotr Pokrzywa Ewa Cieślik Magdalena Surma |
| author_facet | Piotr Pokrzywa Ewa Cieślik Magdalena Surma |
| author_sort | Piotr Pokrzywa |
| collection | DOAJ |
| description | Introduction and objective
Mycotoxins are toxic metabolites produced by certain types of fungi, causing pathological changes in humans and animals. The aim of this study was to assess the degree of contamination of selected cereal grains, bran and cereal products intended for children, with mycotoxins using GCxGC-TOF-MS technique. The study involved mycotoxins belonging to the type A and B trichothecenes group, including T-2 toxin (T-2), HT-2 toxin (HT-2), scirpenol (SCI), 15-monoacetoxyscirpenol (15-MAS), diacetoxyscirpenol (DAS), triacetoxyscirpenol (TAS), fusarenon-X (FUS-X), nivalenol (NIV), deoxynivalenol (DON), 3-acetyl-DON (3-Ac-DON), 15-acetyl-DON (15-Ac-DON). The study also assessed the effect of conditions in which the samples were stored, including temperature (6°C and 28°C) and time (14 and 28 days), on fungal growth and mycotoxin production. Among all studied compounds, only DAS and HT-2 toxins were detected in tested samples, with the exception of products intended for children. Measured HT-2 mycotoxin content in tested samples was in the range 83.9 – 196.4 µg kg -1 .
Results and conclusions
Experiments with storage conditions showed a statistically significant increase in the HT-2 toxin level after 14 days of storage in all samples, irrespective of temperature. Prolonged storage (additional 14 days) did not cause significant changes in the HT-2 content. Further analyses showed a statistically significant effect of storage temperature on HT-2 toxin levels only in cereal products intended for children after both 14 and 28 days. Interestingly, lower temperature (6°C) was more optimal then higher temperature (28°C) for the HT-2 toxin production. No significant effect of storage temperature on HT-2 level was observed for cereal grains and bran. |
| first_indexed | 2024-12-11T18:31:12Z |
| format | Article |
| id | doaj.art-036dd84f9c97496681a53f9b789e8584 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-11T18:31:12Z |
| publishDate | 2019-06-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-036dd84f9c97496681a53f9b789e85842022-12-22T00:54:55ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632019-06-0126226026510.26444/aaem/103398103398Effect of storage conditions on the formation of type A and B trichothecenes in cereal productsPiotr Pokrzywa0Ewa Cieślik1Magdalena Surma2Department of Food Hygiene, Nutrition and Teaching Processes, Regional Sanitary-Epidemiological Station, Krakow, PolandMalopolska Centre of Food Monitoring, Faculty of Food Technology, University of Agriculture, Krakow, PolandMalopolska Centre of Food Monitoring, Faculty of Food Technology, University of Agriculture, Krakow, PolandIntroduction and objective Mycotoxins are toxic metabolites produced by certain types of fungi, causing pathological changes in humans and animals. The aim of this study was to assess the degree of contamination of selected cereal grains, bran and cereal products intended for children, with mycotoxins using GCxGC-TOF-MS technique. The study involved mycotoxins belonging to the type A and B trichothecenes group, including T-2 toxin (T-2), HT-2 toxin (HT-2), scirpenol (SCI), 15-monoacetoxyscirpenol (15-MAS), diacetoxyscirpenol (DAS), triacetoxyscirpenol (TAS), fusarenon-X (FUS-X), nivalenol (NIV), deoxynivalenol (DON), 3-acetyl-DON (3-Ac-DON), 15-acetyl-DON (15-Ac-DON). The study also assessed the effect of conditions in which the samples were stored, including temperature (6°C and 28°C) and time (14 and 28 days), on fungal growth and mycotoxin production. Among all studied compounds, only DAS and HT-2 toxins were detected in tested samples, with the exception of products intended for children. Measured HT-2 mycotoxin content in tested samples was in the range 83.9 – 196.4 µg kg -1 . Results and conclusions Experiments with storage conditions showed a statistically significant increase in the HT-2 toxin level after 14 days of storage in all samples, irrespective of temperature. Prolonged storage (additional 14 days) did not cause significant changes in the HT-2 content. Further analyses showed a statistically significant effect of storage temperature on HT-2 toxin levels only in cereal products intended for children after both 14 and 28 days. Interestingly, lower temperature (6°C) was more optimal then higher temperature (28°C) for the HT-2 toxin production. No significant effect of storage temperature on HT-2 level was observed for cereal grains and bran.http://www.journalssystem.com/aaem/The-effect-of-storage-conditions-on-the-formation-of-type-A-and-B-trichothecenes,103398,0,2.htmltrichothecenescerealscereal productsmycotoxin production |
| spellingShingle | Piotr Pokrzywa Ewa Cieślik Magdalena Surma Effect of storage conditions on the formation of type A and B trichothecenes in cereal products Annals of Agricultural and Environmental Medicine trichothecenes cereals cereal products mycotoxin production |
| title | Effect of storage conditions on the formation of type A and B trichothecenes in cereal products |
| title_full | Effect of storage conditions on the formation of type A and B trichothecenes in cereal products |
| title_fullStr | Effect of storage conditions on the formation of type A and B trichothecenes in cereal products |
| title_full_unstemmed | Effect of storage conditions on the formation of type A and B trichothecenes in cereal products |
| title_short | Effect of storage conditions on the formation of type A and B trichothecenes in cereal products |
| title_sort | effect of storage conditions on the formation of type a and b trichothecenes in cereal products |
| topic | trichothecenes cereals cereal products mycotoxin production |
| url | http://www.journalssystem.com/aaem/The-effect-of-storage-conditions-on-the-formation-of-type-A-and-B-trichothecenes,103398,0,2.html |
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