Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in <i>Daphnia magna</i>
Bisphenols are used in the production of polycarbonate plastics and epoxy resins. Bisphenol A (BPA) has been widely studied and is believed to act as an endocrine disruptor. Bisphenol F (BPF) and bisphenol S (BPS) have increasingly been employed as replacements for BPA, although previous studies sug...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/10/666 |
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| author | Erico A. Oliveira Pereira Lisa M. Labine Sonya Kleywegt Karl J. Jobst André J. Simpson Myrna J. Simpson |
| author_facet | Erico A. Oliveira Pereira Lisa M. Labine Sonya Kleywegt Karl J. Jobst André J. Simpson Myrna J. Simpson |
| author_sort | Erico A. Oliveira Pereira |
| collection | DOAJ |
| description | Bisphenols are used in the production of polycarbonate plastics and epoxy resins. Bisphenol A (BPA) has been widely studied and is believed to act as an endocrine disruptor. Bisphenol F (BPF) and bisphenol S (BPS) have increasingly been employed as replacements for BPA, although previous studies suggested that they yield similar physiological responses to several organisms. <i>Daphnia magna</i> is a common model organism for ecotoxicology and was exposed to sub-lethal concentrations of BPA, BPF, and BPS to investigate disruption to metabolic profiles. Targeted metabolite analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to measure polar metabolites extracted from <i>D. magna</i>, which are linked to a range of biochemical pathways. Multivariate analyses and individual metabolite changes showed similar non-monotonic concentration responses for all three bisphenols (BPA, BPF, and BPS). Pathway analyses indicated the perturbation of similar and distinct pathways, mostly associated with protein synthesis, amino acid metabolism, and energy metabolism. Overall, we observed responses that can be linked to a chemical class (bisphenols) as well as distinct responses that can be related to each individual bisphenol type (A, F, and S). These findings further demonstrate the need for using metabolomic analyses in exposure assessment, especially for chemicals within the same class which may disrupt the biochemistry uniquely at the molecular-level. |
| first_indexed | 2024-03-10T06:23:37Z |
| format | Article |
| id | doaj.art-036f8246bbb741509a6a5f06f539343c |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T06:23:37Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-036f8246bbb741509a6a5f06f539343c2023-11-22T19:07:05ZengMDPI AGMetabolites2218-19892021-09-01111066610.3390/metabo11100666Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in <i>Daphnia magna</i>Erico A. Oliveira Pereira0Lisa M. Labine1Sonya Kleywegt2Karl J. Jobst3André J. Simpson4Myrna J. Simpson5Environmental NMR Centre and Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaEnvironmental NMR Centre and Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaTechnical Assessment and Standards Development Branch, Ontario Ministry of the Environment, Conservation and Parks, Toronto, ON M4V 1M2, CanadaDepartment of Chemistry, Memorial University of Newfoundland, St. John’s, NL A1B 3X7, CanadaEnvironmental NMR Centre and Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaEnvironmental NMR Centre and Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaBisphenols are used in the production of polycarbonate plastics and epoxy resins. Bisphenol A (BPA) has been widely studied and is believed to act as an endocrine disruptor. Bisphenol F (BPF) and bisphenol S (BPS) have increasingly been employed as replacements for BPA, although previous studies suggested that they yield similar physiological responses to several organisms. <i>Daphnia magna</i> is a common model organism for ecotoxicology and was exposed to sub-lethal concentrations of BPA, BPF, and BPS to investigate disruption to metabolic profiles. Targeted metabolite analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to measure polar metabolites extracted from <i>D. magna</i>, which are linked to a range of biochemical pathways. Multivariate analyses and individual metabolite changes showed similar non-monotonic concentration responses for all three bisphenols (BPA, BPF, and BPS). Pathway analyses indicated the perturbation of similar and distinct pathways, mostly associated with protein synthesis, amino acid metabolism, and energy metabolism. Overall, we observed responses that can be linked to a chemical class (bisphenols) as well as distinct responses that can be related to each individual bisphenol type (A, F, and S). These findings further demonstrate the need for using metabolomic analyses in exposure assessment, especially for chemicals within the same class which may disrupt the biochemistry uniquely at the molecular-level.https://www.mdpi.com/2218-1989/11/10/666ecotoxicologymetabolomicsbisphenol pollutantsbisphenol A (BPA)bisphenol F (BPF)bisphenol S (BPS) |
| spellingShingle | Erico A. Oliveira Pereira Lisa M. Labine Sonya Kleywegt Karl J. Jobst André J. Simpson Myrna J. Simpson Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in <i>Daphnia magna</i> Metabolites ecotoxicology metabolomics bisphenol pollutants bisphenol A (BPA) bisphenol F (BPF) bisphenol S (BPS) |
| title | Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in <i>Daphnia magna</i> |
| title_full | Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in <i>Daphnia magna</i> |
| title_fullStr | Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in <i>Daphnia magna</i> |
| title_full_unstemmed | Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in <i>Daphnia magna</i> |
| title_short | Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in <i>Daphnia magna</i> |
| title_sort | metabolomics reveals that bisphenol pollutants impair protein synthesis related pathways in i daphnia magna i |
| topic | ecotoxicology metabolomics bisphenol pollutants bisphenol A (BPA) bisphenol F (BPF) bisphenol S (BPS) |
| url | https://www.mdpi.com/2218-1989/11/10/666 |
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