New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy
The aim of the present work was to obtain drug-loaded hydrogels based on combinations of dextran, chitosan/gelatin/xanthan, and poly (acrylamide) as a sustained and controlled release vehicle of Doxorubicin, a drug used in skin cancer therapy that is associated with severe side effects. Hydrogels fo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/5/371 |
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| author | Andreea Luca Isabella Nacu Sabina Tanasache Cătălina Anişoara Peptu Maria Butnaru Liliana Verestiuc |
| author_facet | Andreea Luca Isabella Nacu Sabina Tanasache Cătălina Anişoara Peptu Maria Butnaru Liliana Verestiuc |
| author_sort | Andreea Luca |
| collection | DOAJ |
| description | The aim of the present work was to obtain drug-loaded hydrogels based on combinations of dextran, chitosan/gelatin/xanthan, and poly (acrylamide) as a sustained and controlled release vehicle of Doxorubicin, a drug used in skin cancer therapy that is associated with severe side effects. Hydrogels for use as 3D hydrophilic networks with good manipulation characteristics were produced using methacrylated biopolymer derivatives and the methacrylate group’s polymerization with synthetic monomers in the presence of a photo-initiator, under UV light stimulation (365 nm). Transformed infrared spectroscopy analysis (FT-IR) confirmed the hydrogels’ network structure (natural–synthetic composition and photocrosslinking), while scanning electron microscopy (SEM) analysis confirmed the microporous morphology. The hydrogels are swellable in simulated biological fluids and the material’s morphology regulates the swelling properties: the maximum swelling degree was obtained for dextran–chitosan-based hydrogels because of their higher porosity and pore distribution. The hydrogels are bioadhesive on a biological simulating membrane, and values for the force of detachment and work of adhesion are recommended for applications on skin tissue. The Doxorubicin was loaded into the hydrogels and the drug was released by diffusion for all the resulting hydrogels, with small contributions from the hydrogel networks’ relaxation. Doxorubicin-loaded hydrogels are efficient on keratinocytes tumor cells, the sustained released drug interrupting the cells’ division and inducing cell apoptosis; we recommend the obtained materials for the topical treatment of cutaneous squamous cell carcinoma. |
| first_indexed | 2024-03-11T03:42:08Z |
| format | Article |
| id | doaj.art-03702ae4b1b94e3598fe865ed8667746 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-11T03:42:08Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-03702ae4b1b94e3598fe865ed86677462023-11-18T01:27:35ZengMDPI AGGels2310-28612023-05-019537110.3390/gels9050371New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer TherapyAndreea Luca0Isabella Nacu1Sabina Tanasache2Cătălina Anişoara Peptu3Maria Butnaru4Liliana Verestiuc5Department of Biomedical Sciences, Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaDepartment of Biomedical Sciences, Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaDepartment of Biomedical Sciences, Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaCristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iaşi, 700050 Iasi, RomaniaDepartment of Biomedical Sciences, Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaDepartment of Biomedical Sciences, Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaThe aim of the present work was to obtain drug-loaded hydrogels based on combinations of dextran, chitosan/gelatin/xanthan, and poly (acrylamide) as a sustained and controlled release vehicle of Doxorubicin, a drug used in skin cancer therapy that is associated with severe side effects. Hydrogels for use as 3D hydrophilic networks with good manipulation characteristics were produced using methacrylated biopolymer derivatives and the methacrylate group’s polymerization with synthetic monomers in the presence of a photo-initiator, under UV light stimulation (365 nm). Transformed infrared spectroscopy analysis (FT-IR) confirmed the hydrogels’ network structure (natural–synthetic composition and photocrosslinking), while scanning electron microscopy (SEM) analysis confirmed the microporous morphology. The hydrogels are swellable in simulated biological fluids and the material’s morphology regulates the swelling properties: the maximum swelling degree was obtained for dextran–chitosan-based hydrogels because of their higher porosity and pore distribution. The hydrogels are bioadhesive on a biological simulating membrane, and values for the force of detachment and work of adhesion are recommended for applications on skin tissue. The Doxorubicin was loaded into the hydrogels and the drug was released by diffusion for all the resulting hydrogels, with small contributions from the hydrogel networks’ relaxation. Doxorubicin-loaded hydrogels are efficient on keratinocytes tumor cells, the sustained released drug interrupting the cells’ division and inducing cell apoptosis; we recommend the obtained materials for the topical treatment of cutaneous squamous cell carcinoma.https://www.mdpi.com/2310-2861/9/5/371hydrogelsbiopolymerspoly(acrylamide)Doxorubicinskin cancer therapy |
| spellingShingle | Andreea Luca Isabella Nacu Sabina Tanasache Cătălina Anişoara Peptu Maria Butnaru Liliana Verestiuc New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy Gels hydrogels biopolymers poly(acrylamide) Doxorubicin skin cancer therapy |
| title | New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy |
| title_full | New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy |
| title_fullStr | New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy |
| title_full_unstemmed | New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy |
| title_short | New Methacrylated Biopolymer-Based Hydrogels as Localized Drug Delivery Systems in Skin Cancer Therapy |
| title_sort | new methacrylated biopolymer based hydrogels as localized drug delivery systems in skin cancer therapy |
| topic | hydrogels biopolymers poly(acrylamide) Doxorubicin skin cancer therapy |
| url | https://www.mdpi.com/2310-2861/9/5/371 |
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