The Role of Pyoluteorin from <i>Pseudomonas protegens</i> Pf-5 in Suppressing the Growth and Pathogenicity of <i>Pantoea ananatis</i> on Maize
The rhizospheric bacterium <i>Pseudomonas protegens</i> Pf-5 can colonize the seed and root surfaces of plants, and can protect them from pathogen infection. Secondary metabolites, including lipopeptides and polyketides produced by Pf-5, are involved in its biocontrol activity. We isolat...
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6431 |
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| author | Qin Gu Junqing Qiao Ruoyi Wang Juan Lu Zhengqi Wang Pingping Li Lulu Zhang Qurban Ali Abdur Rashid Khan Xuewen Gao Huijun Wu |
| author_facet | Qin Gu Junqing Qiao Ruoyi Wang Juan Lu Zhengqi Wang Pingping Li Lulu Zhang Qurban Ali Abdur Rashid Khan Xuewen Gao Huijun Wu |
| author_sort | Qin Gu |
| collection | DOAJ |
| description | The rhizospheric bacterium <i>Pseudomonas protegens</i> Pf-5 can colonize the seed and root surfaces of plants, and can protect them from pathogen infection. Secondary metabolites, including lipopeptides and polyketides produced by Pf-5, are involved in its biocontrol activity. We isolated a crude extract from Pf-5. It exhibited significant surface activity and strong antibacterial activity against <i>Pantoea ananatis</i> DZ-12, which causes maize brown rot on leaves. HPLC analysis combined with activity tests showed that the polyketide pyoluteorin in the crude extract participated in the suppression of DZ-12 growth, and that the lipopeptide orfamide A was the major biosurfactant in the crude extract. Further studies indicated that the pyoluteorin in the crude extract significantly suppressed the biofilm formation of DZ-12, and it induced the accumulation of reactive oxygen species in DZ-12 cells. Scanning electron microscopy and transmission electron microscopy observation revealed that the crude extract severely damaged the pathogen cells and caused cytoplasmic extravasations and hollowing of the cells. The pathogenicity of DZ-12 on maize leaves was significantly reduced by the crude extract from Pf-5 in a dose-dependent manner. The polyketide pyoluteorin had strong antibacterial activity against DZ-12, and it has the potential for development as an antimicrobial agent. |
| first_indexed | 2024-03-09T23:35:40Z |
| format | Article |
| id | doaj.art-03733684868a4b02a3c6e5cd37daa5bc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:35:40Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-03733684868a4b02a3c6e5cd37daa5bc2023-11-23T17:00:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312643110.3390/ijms23126431The Role of Pyoluteorin from <i>Pseudomonas protegens</i> Pf-5 in Suppressing the Growth and Pathogenicity of <i>Pantoea ananatis</i> on MaizeQin Gu0Junqing Qiao1Ruoyi Wang2Juan Lu3Zhengqi Wang4Pingping Li5Lulu Zhang6Qurban Ali7Abdur Rashid Khan8Xuewen Gao9Huijun Wu10Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaThe rhizospheric bacterium <i>Pseudomonas protegens</i> Pf-5 can colonize the seed and root surfaces of plants, and can protect them from pathogen infection. Secondary metabolites, including lipopeptides and polyketides produced by Pf-5, are involved in its biocontrol activity. We isolated a crude extract from Pf-5. It exhibited significant surface activity and strong antibacterial activity against <i>Pantoea ananatis</i> DZ-12, which causes maize brown rot on leaves. HPLC analysis combined with activity tests showed that the polyketide pyoluteorin in the crude extract participated in the suppression of DZ-12 growth, and that the lipopeptide orfamide A was the major biosurfactant in the crude extract. Further studies indicated that the pyoluteorin in the crude extract significantly suppressed the biofilm formation of DZ-12, and it induced the accumulation of reactive oxygen species in DZ-12 cells. Scanning electron microscopy and transmission electron microscopy observation revealed that the crude extract severely damaged the pathogen cells and caused cytoplasmic extravasations and hollowing of the cells. The pathogenicity of DZ-12 on maize leaves was significantly reduced by the crude extract from Pf-5 in a dose-dependent manner. The polyketide pyoluteorin had strong antibacterial activity against DZ-12, and it has the potential for development as an antimicrobial agent.https://www.mdpi.com/1422-0067/23/12/6431<i>Pseudomonas protegens</i><i>Pantoea ananatis</i>pyoluteorinorfamidebiofilmbiocontrol |
| spellingShingle | Qin Gu Junqing Qiao Ruoyi Wang Juan Lu Zhengqi Wang Pingping Li Lulu Zhang Qurban Ali Abdur Rashid Khan Xuewen Gao Huijun Wu The Role of Pyoluteorin from <i>Pseudomonas protegens</i> Pf-5 in Suppressing the Growth and Pathogenicity of <i>Pantoea ananatis</i> on Maize International Journal of Molecular Sciences <i>Pseudomonas protegens</i> <i>Pantoea ananatis</i> pyoluteorin orfamide biofilm biocontrol |
| title | The Role of Pyoluteorin from <i>Pseudomonas protegens</i> Pf-5 in Suppressing the Growth and Pathogenicity of <i>Pantoea ananatis</i> on Maize |
| title_full | The Role of Pyoluteorin from <i>Pseudomonas protegens</i> Pf-5 in Suppressing the Growth and Pathogenicity of <i>Pantoea ananatis</i> on Maize |
| title_fullStr | The Role of Pyoluteorin from <i>Pseudomonas protegens</i> Pf-5 in Suppressing the Growth and Pathogenicity of <i>Pantoea ananatis</i> on Maize |
| title_full_unstemmed | The Role of Pyoluteorin from <i>Pseudomonas protegens</i> Pf-5 in Suppressing the Growth and Pathogenicity of <i>Pantoea ananatis</i> on Maize |
| title_short | The Role of Pyoluteorin from <i>Pseudomonas protegens</i> Pf-5 in Suppressing the Growth and Pathogenicity of <i>Pantoea ananatis</i> on Maize |
| title_sort | role of pyoluteorin from i pseudomonas protegens i pf 5 in suppressing the growth and pathogenicity of i pantoea ananatis i on maize |
| topic | <i>Pseudomonas protegens</i> <i>Pantoea ananatis</i> pyoluteorin orfamide biofilm biocontrol |
| url | https://www.mdpi.com/1422-0067/23/12/6431 |
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