TLR7 Negatively Regulates B10 Cells Predominantly in an IFNγ Signaling Dependent Manner
IL-10 producing B cells (B10 cells) play an important immunoregulatory role in various autoimmune and infection conditions. However, the factors that regulate their development and maintenance are incompletely understood. Recently, we and others have established a requirement for TLR7 in promoting a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.01632/full |
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| author | Sathi Babu Chodisetti Adam J. Fike Phillip P. Domeier Nicholas M. Choi Chetna Soni Ziaur S. M. Rahman |
| author_facet | Sathi Babu Chodisetti Adam J. Fike Phillip P. Domeier Nicholas M. Choi Chetna Soni Ziaur S. M. Rahman |
| author_sort | Sathi Babu Chodisetti |
| collection | DOAJ |
| description | IL-10 producing B cells (B10 cells) play an important immunoregulatory role in various autoimmune and infection conditions. However, the factors that regulate their development and maintenance are incompletely understood. Recently, we and others have established a requirement for TLR7 in promoting autoimmune antibody forming cell (AFC) and germinal center (GC) responses. Here we report an important additional role of TLR7 in the negative regulation of B10 cell development. TLR7 overexpression or overstimulation promoted the reduction of B10 cells whereas TLR7 deficiency rescued these cells in both non-autoimmune and autoimmune-prone mice. TLR7 expression was further inversely correlated with B cell-dependent IL-10 production and its inhibition of CD4 T cell proliferation and IFNγ production in an in vitro B cell and T cell co-culture system. Further, B10 cells displayed elevated TLR7, IFNγR, and STAT1 expression compared to non-B10 cells. Interestingly, deficiency of IFNγR in TLR7 overexpressing lupus-prone mice rescued B10 cells from TLR7-mediated reduction. Finally, B cell intrinsic deletion of IFNγR was sufficient to restore B10 cells in the spleens of TLR7-promoted autoimmune mouse model. In conclusion, our findings demonstrate a novel role for the IFNγR-STAT1 pathway in TLR7-mediated negative regulation of B10 cell development. |
| first_indexed | 2024-04-13T16:51:41Z |
| format | Article |
| id | doaj.art-0379784cda97449b9d4f6aff24fdd7f5 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T16:51:41Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-0379784cda97449b9d4f6aff24fdd7f52022-12-22T02:38:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-07-011110.3389/fimmu.2020.01632554683TLR7 Negatively Regulates B10 Cells Predominantly in an IFNγ Signaling Dependent MannerSathi Babu ChodisettiAdam J. FikePhillip P. DomeierNicholas M. ChoiChetna SoniZiaur S. M. RahmanIL-10 producing B cells (B10 cells) play an important immunoregulatory role in various autoimmune and infection conditions. However, the factors that regulate their development and maintenance are incompletely understood. Recently, we and others have established a requirement for TLR7 in promoting autoimmune antibody forming cell (AFC) and germinal center (GC) responses. Here we report an important additional role of TLR7 in the negative regulation of B10 cell development. TLR7 overexpression or overstimulation promoted the reduction of B10 cells whereas TLR7 deficiency rescued these cells in both non-autoimmune and autoimmune-prone mice. TLR7 expression was further inversely correlated with B cell-dependent IL-10 production and its inhibition of CD4 T cell proliferation and IFNγ production in an in vitro B cell and T cell co-culture system. Further, B10 cells displayed elevated TLR7, IFNγR, and STAT1 expression compared to non-B10 cells. Interestingly, deficiency of IFNγR in TLR7 overexpressing lupus-prone mice rescued B10 cells from TLR7-mediated reduction. Finally, B cell intrinsic deletion of IFNγR was sufficient to restore B10 cells in the spleens of TLR7-promoted autoimmune mouse model. In conclusion, our findings demonstrate a novel role for the IFNγR-STAT1 pathway in TLR7-mediated negative regulation of B10 cell development.https://www.frontiersin.org/article/10.3389/fimmu.2020.01632/fulltoll-like receptor 7B10 cellsB regulatory cellsIFNGRlupusautoimmunity |
| spellingShingle | Sathi Babu Chodisetti Adam J. Fike Phillip P. Domeier Nicholas M. Choi Chetna Soni Ziaur S. M. Rahman TLR7 Negatively Regulates B10 Cells Predominantly in an IFNγ Signaling Dependent Manner Frontiers in Immunology toll-like receptor 7 B10 cells B regulatory cells IFNGR lupus autoimmunity |
| title | TLR7 Negatively Regulates B10 Cells Predominantly in an IFNγ Signaling Dependent Manner |
| title_full | TLR7 Negatively Regulates B10 Cells Predominantly in an IFNγ Signaling Dependent Manner |
| title_fullStr | TLR7 Negatively Regulates B10 Cells Predominantly in an IFNγ Signaling Dependent Manner |
| title_full_unstemmed | TLR7 Negatively Regulates B10 Cells Predominantly in an IFNγ Signaling Dependent Manner |
| title_short | TLR7 Negatively Regulates B10 Cells Predominantly in an IFNγ Signaling Dependent Manner |
| title_sort | tlr7 negatively regulates b10 cells predominantly in an ifnγ signaling dependent manner |
| topic | toll-like receptor 7 B10 cells B regulatory cells IFNGR lupus autoimmunity |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.01632/full |
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