Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters
The basic principles of ultrafast plasmonic PCR have been promulgated in the scientific and technological literature for over a decade. Yet, its everyday diagnostic utility remains unvalidated in pre-clinical and clinical settings. Although the impressive speed of plasmonic PCR reaction is well-docu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/14/5/477 |
| _version_ | 1797264688494411776 |
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| author | Ivan Brukner Miltiadis Paliouras Mark Trifiro Marc Bohbot Daniel Shamir Andrew G. Kirk |
| author_facet | Ivan Brukner Miltiadis Paliouras Mark Trifiro Marc Bohbot Daniel Shamir Andrew G. Kirk |
| author_sort | Ivan Brukner |
| collection | DOAJ |
| description | The basic principles of ultrafast plasmonic PCR have been promulgated in the scientific and technological literature for over a decade. Yet, its everyday diagnostic utility remains unvalidated in pre-clinical and clinical settings. Although the impressive speed of plasmonic PCR reaction is well-documented, implementing this process into a device form compatible with routine diagnostic tasks has been challenging. Here, we show that combining careful system engineering and process control with innovative and specific PCR biochemistry makes it possible to routinely achieve a sensitive and robust “10 min” PCR assay in a compact and lightweight system. The critical analytical parameters of PCR reactions are discussed in the current instrument setting. |
| first_indexed | 2024-04-25T00:32:53Z |
| format | Article |
| id | doaj.art-038142ebc8844a629284a5758e8440b5 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-04-25T00:32:53Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-038142ebc8844a629284a5758e8440b52024-03-12T16:41:54ZengMDPI AGDiagnostics2075-44182024-02-0114547710.3390/diagnostics14050477Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical ParametersIvan Brukner0Miltiadis Paliouras1Mark Trifiro2Marc Bohbot3Daniel Shamir4Andrew G. Kirk5Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, CanadaDepartment of Medicine, McGill University, Montreal, QC H3G 2M1, CanadaLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, CanadaNexless Healthcare, 1315 Chem. Canora, Mont-Royal, Montreal, QC H3P 2J5, CanadaNexless Healthcare, 1315 Chem. Canora, Mont-Royal, Montreal, QC H3P 2J5, CanadaDepartment of Electrical and Computer Engineering, McGill University, Montreal, QC H3A 0E9, CanadaThe basic principles of ultrafast plasmonic PCR have been promulgated in the scientific and technological literature for over a decade. Yet, its everyday diagnostic utility remains unvalidated in pre-clinical and clinical settings. Although the impressive speed of plasmonic PCR reaction is well-documented, implementing this process into a device form compatible with routine diagnostic tasks has been challenging. Here, we show that combining careful system engineering and process control with innovative and specific PCR biochemistry makes it possible to routinely achieve a sensitive and robust “10 min” PCR assay in a compact and lightweight system. The critical analytical parameters of PCR reactions are discussed in the current instrument setting.https://www.mdpi.com/2075-4418/14/5/477PCRultrafastplasmonic PCRmoleculardiagnosticsDNA |
| spellingShingle | Ivan Brukner Miltiadis Paliouras Mark Trifiro Marc Bohbot Daniel Shamir Andrew G. Kirk Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters Diagnostics PCR ultrafast plasmonic PCR molecular diagnostics DNA |
| title | Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters |
| title_full | Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters |
| title_fullStr | Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters |
| title_full_unstemmed | Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters |
| title_short | Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters |
| title_sort | assessing different pcr master mixes for ultrarapid dna amplification important analytical parameters |
| topic | PCR ultrafast plasmonic PCR molecular diagnostics DNA |
| url | https://www.mdpi.com/2075-4418/14/5/477 |
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