Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes
Mesophilic and thermophilic enzyme counterparts are often studied to understand how proteins function under harsh conditions. To function well outside of standard temperature ranges, thermophiles often tightly regulate their structural ensemble through intra-protein communication (via allostery) and...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1281062/full |
| _version_ | 1797663313150083072 |
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| author | Alexa L. Knight Vinnie Widjaja George P. Lisi |
| author_facet | Alexa L. Knight Vinnie Widjaja George P. Lisi |
| author_sort | Alexa L. Knight |
| collection | DOAJ |
| description | Mesophilic and thermophilic enzyme counterparts are often studied to understand how proteins function under harsh conditions. To function well outside of standard temperature ranges, thermophiles often tightly regulate their structural ensemble through intra-protein communication (via allostery) and altered interactions with ligands. It has also become apparent in recent years that the enhancement or diminution of allosteric crosstalk can be temperature-dependent and distinguish thermophilic enzymes from their mesophilic paralogs. Since most studies of allostery utilize chemical modifications from pH, mutations, or ligands, the impact of temperature on allosteric function is comparatively understudied. Here, we discuss the biophysical methods, as well as critical case studies, that dissect temperature-dependent function of mesophilic-thermophilic enzyme pairs and their allosteric regulation across a range of temperatures. |
| first_indexed | 2024-03-11T19:12:42Z |
| format | Article |
| id | doaj.art-0384456f4c4841719588498e970f4c09 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-03-11T19:12:42Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-0384456f4c4841719588498e970f4c092023-10-09T10:28:56ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2023-10-011010.3389/fmolb.2023.12810621281062Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymesAlexa L. KnightVinnie WidjajaGeorge P. LisiMesophilic and thermophilic enzyme counterparts are often studied to understand how proteins function under harsh conditions. To function well outside of standard temperature ranges, thermophiles often tightly regulate their structural ensemble through intra-protein communication (via allostery) and altered interactions with ligands. It has also become apparent in recent years that the enhancement or diminution of allosteric crosstalk can be temperature-dependent and distinguish thermophilic enzymes from their mesophilic paralogs. Since most studies of allostery utilize chemical modifications from pH, mutations, or ligands, the impact of temperature on allosteric function is comparatively understudied. Here, we discuss the biophysical methods, as well as critical case studies, that dissect temperature-dependent function of mesophilic-thermophilic enzyme pairs and their allosteric regulation across a range of temperatures.https://www.frontiersin.org/articles/10.3389/fmolb.2023.1281062/fullNMRthermodynamicskineticstemperatureallosterythermophiles |
| spellingShingle | Alexa L. Knight Vinnie Widjaja George P. Lisi Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes Frontiers in Molecular Biosciences NMR thermodynamics kinetics temperature allostery thermophiles |
| title | Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes |
| title_full | Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes |
| title_fullStr | Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes |
| title_full_unstemmed | Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes |
| title_short | Temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes |
| title_sort | temperature as a modulator of allosteric motions and crosstalk in mesophilic and thermophilic enzymes |
| topic | NMR thermodynamics kinetics temperature allostery thermophiles |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1281062/full |
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