Severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornis
Abstract Background Ticks serve as vectors for a diverse array of pathogens, including viruses responsible for both human and livestock diseases. Symbiotic bacteria hold significant potential for controlling tick-borne disease. However, the alteration of tick gut bacterial community in response to p...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-024-06204-w |
| _version_ | 1797275658132389888 |
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| author | Yu Sun Chen Chen Chenghong Zeng Qianfeng Xia Chuanfei Yuan Hua Pei |
| author_facet | Yu Sun Chen Chen Chenghong Zeng Qianfeng Xia Chuanfei Yuan Hua Pei |
| author_sort | Yu Sun |
| collection | DOAJ |
| description | Abstract Background Ticks serve as vectors for a diverse array of pathogens, including viruses responsible for both human and livestock diseases. Symbiotic bacteria hold significant potential for controlling tick-borne disease. However, the alteration of tick gut bacterial community in response to pathogen infection has not been analyzed for any tick-borne viruses. Here, the impact of severe fever with thrombocytopenia syndrome virus (SFTSV) infection on bacterial diversity in the gut of Haemaphysalis longicornis is investigated. Methods Unfed tick females were artificially infected with SFTSV. The gut samples were collected and the genomic DNA was extracted. We then investigated alterations in gut bacterial composition in response to SFTSV infection through 16S rRNA gene sequencing. Results The study found that a reduction in the number of operational taxonomic units (OTUs) in the tick gut following SFTSV infection. However, there were no significant changes in alpha diversity indices upon infection. Four genera, including Corynebacterium, Arthrobacter, Sphingomonas, and Escherichia, were identified as biomarkers for the tick gut without SFTSV infection. Notably, the predicted correlation network indicated that the biomarkers Sphingomonas and Escherichia exhibited positive correlations within the same subcommunity, which was altered upon viral infection. Conclusions These findings revealed that the change in tick gut bacterial composition upon SFTSV infection and could facilitate the discovery new target for tick-borne viral disease control. Graphical Abstract |
| first_indexed | 2024-03-07T15:17:37Z |
| format | Article |
| id | doaj.art-03868b1b9c6548c48545a2d89993f552 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-07T15:17:37Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-03868b1b9c6548c48545a2d89993f5522024-03-05T17:50:39ZengBMCParasites & Vectors1756-33052024-03-011711910.1186/s13071-024-06204-wSevere fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornisYu Sun0Chen Chen1Chenghong Zeng2Qianfeng Xia3Chuanfei Yuan4Hua Pei5Key Laboratory of Tropical Translational Medicine of Ministry of Education, International School of Public Health and One Health, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, The Second Affiliated Hospital, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, The Second Affiliated Hospital, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, International School of Public Health and One Health, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, The Second Affiliated Hospital, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, The Second Affiliated Hospital, Hainan Medical UniversityAbstract Background Ticks serve as vectors for a diverse array of pathogens, including viruses responsible for both human and livestock diseases. Symbiotic bacteria hold significant potential for controlling tick-borne disease. However, the alteration of tick gut bacterial community in response to pathogen infection has not been analyzed for any tick-borne viruses. Here, the impact of severe fever with thrombocytopenia syndrome virus (SFTSV) infection on bacterial diversity in the gut of Haemaphysalis longicornis is investigated. Methods Unfed tick females were artificially infected with SFTSV. The gut samples were collected and the genomic DNA was extracted. We then investigated alterations in gut bacterial composition in response to SFTSV infection through 16S rRNA gene sequencing. Results The study found that a reduction in the number of operational taxonomic units (OTUs) in the tick gut following SFTSV infection. However, there were no significant changes in alpha diversity indices upon infection. Four genera, including Corynebacterium, Arthrobacter, Sphingomonas, and Escherichia, were identified as biomarkers for the tick gut without SFTSV infection. Notably, the predicted correlation network indicated that the biomarkers Sphingomonas and Escherichia exhibited positive correlations within the same subcommunity, which was altered upon viral infection. Conclusions These findings revealed that the change in tick gut bacterial composition upon SFTSV infection and could facilitate the discovery new target for tick-borne viral disease control. Graphical Abstracthttps://doi.org/10.1186/s13071-024-06204-wSFTSVGut microbiotaTicksHaemaphysalis longicornisSphingomonas |
| spellingShingle | Yu Sun Chen Chen Chenghong Zeng Qianfeng Xia Chuanfei Yuan Hua Pei Severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornis Parasites & Vectors SFTSV Gut microbiota Ticks Haemaphysalis longicornis Sphingomonas |
| title | Severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornis |
| title_full | Severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornis |
| title_fullStr | Severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornis |
| title_full_unstemmed | Severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornis |
| title_short | Severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector Haemaphysalis longicornis |
| title_sort | severe fever with thrombocytopenia syndrome virus infection shapes gut microbiome of the tick vector haemaphysalis longicornis |
| topic | SFTSV Gut microbiota Ticks Haemaphysalis longicornis Sphingomonas |
| url | https://doi.org/10.1186/s13071-024-06204-w |
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