Rapid visualization of latent fingermarks using gold seed-mediated enhancement
Abstract Background Fingermarks are one of the most important and useful forms of physical evidence in forensic investigations. However, latent fingermarks are not directly visible, but can be visualized due to the presence of other residues (such as inorganic salts, proteins, polypeptides, enzymes...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2016-11-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-016-0228-3 |
| _version_ | 1828390395255455744 |
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| author | Chia-Hao Su Chun-Chieh Yu Fong-Yu Cheng |
| author_facet | Chia-Hao Su Chun-Chieh Yu Fong-Yu Cheng |
| author_sort | Chia-Hao Su |
| collection | DOAJ |
| description | Abstract Background Fingermarks are one of the most important and useful forms of physical evidence in forensic investigations. However, latent fingermarks are not directly visible, but can be visualized due to the presence of other residues (such as inorganic salts, proteins, polypeptides, enzymes and human metabolites) which can be detected or recognized through various strategies. Convenient and rapid techniques are still needed to provide obvious contrast between the background and the fingermark ridges and to then visualize latent fingermark with a high degree of selectivity and sensitivity. Results In this work, lysozyme-binding aptamer-conjugated Au nanoparticles (NPs) are used to recognize and target lysozyme in the fingermark ridges, and Au+-complex solution is used as a growth agent to reduce Au+ from Au+ to Au0 on the surface of the Au NPs. Distinct fingermark patterns were visualized on a range of professional forensic within 3 min; the resulting images could be observed by the naked eye without background interference. The entire processes from fingermark collection to visualization only entails two steps and can be completed in less than 10 min. The proposed method provides cost and time savings over current fingermark visualization methods. Conclusions We report a simple, inexpensive, and fast method for the rapid visualization of latent fingermarks on the non-porous substrates using Au seed-mediated enhancement. Au seed-mediated enhancement is used to achieve the rapid visualization of latent fingermarks on non-porous substrates by the naked eye without the use of expensive or sophisticated instruments. The proposed approach offers faster detection and visualization of latent fingermarks than existing methods. The proposed method is expected to increase detection efficiency for latent fingermarks and reduce time requirements and costs for forensic investigations. |
| first_indexed | 2024-12-10T06:43:22Z |
| format | Article |
| id | doaj.art-03890127b2024ad5b67864629208791f |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-12-10T06:43:22Z |
| publishDate | 2016-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-03890127b2024ad5b67864629208791f2022-12-22T01:58:43ZengBMCJournal of Nanobiotechnology1477-31552016-11-0114111410.1186/s12951-016-0228-3Rapid visualization of latent fingermarks using gold seed-mediated enhancementChia-Hao Su0Chun-Chieh Yu1Fong-Yu Cheng2Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial HospitalInstitute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial HospitalDepartment of Chemistry, Chinese Culture UniversityAbstract Background Fingermarks are one of the most important and useful forms of physical evidence in forensic investigations. However, latent fingermarks are not directly visible, but can be visualized due to the presence of other residues (such as inorganic salts, proteins, polypeptides, enzymes and human metabolites) which can be detected or recognized through various strategies. Convenient and rapid techniques are still needed to provide obvious contrast between the background and the fingermark ridges and to then visualize latent fingermark with a high degree of selectivity and sensitivity. Results In this work, lysozyme-binding aptamer-conjugated Au nanoparticles (NPs) are used to recognize and target lysozyme in the fingermark ridges, and Au+-complex solution is used as a growth agent to reduce Au+ from Au+ to Au0 on the surface of the Au NPs. Distinct fingermark patterns were visualized on a range of professional forensic within 3 min; the resulting images could be observed by the naked eye without background interference. The entire processes from fingermark collection to visualization only entails two steps and can be completed in less than 10 min. The proposed method provides cost and time savings over current fingermark visualization methods. Conclusions We report a simple, inexpensive, and fast method for the rapid visualization of latent fingermarks on the non-porous substrates using Au seed-mediated enhancement. Au seed-mediated enhancement is used to achieve the rapid visualization of latent fingermarks on non-porous substrates by the naked eye without the use of expensive or sophisticated instruments. The proposed approach offers faster detection and visualization of latent fingermarks than existing methods. The proposed method is expected to increase detection efficiency for latent fingermarks and reduce time requirements and costs for forensic investigations.http://link.springer.com/article/10.1186/s12951-016-0228-3AptamersAu nanoparticlesAu seedsFingermarksLysozyme |
| spellingShingle | Chia-Hao Su Chun-Chieh Yu Fong-Yu Cheng Rapid visualization of latent fingermarks using gold seed-mediated enhancement Journal of Nanobiotechnology Aptamers Au nanoparticles Au seeds Fingermarks Lysozyme |
| title | Rapid visualization of latent fingermarks using gold seed-mediated enhancement |
| title_full | Rapid visualization of latent fingermarks using gold seed-mediated enhancement |
| title_fullStr | Rapid visualization of latent fingermarks using gold seed-mediated enhancement |
| title_full_unstemmed | Rapid visualization of latent fingermarks using gold seed-mediated enhancement |
| title_short | Rapid visualization of latent fingermarks using gold seed-mediated enhancement |
| title_sort | rapid visualization of latent fingermarks using gold seed mediated enhancement |
| topic | Aptamers Au nanoparticles Au seeds Fingermarks Lysozyme |
| url | http://link.springer.com/article/10.1186/s12951-016-0228-3 |
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